U23. Assisted Reproduction

U23-S09. Quality control for Human Assisted Reproduction clinics

Quality control for Human Assisted Reproduction clinics

The assisted reproduction unit has a laboratory with extensive equipment with which to carry out the basic assisted reproduction techniques, among which are extraction of gametes (ovum and sperm), extraction of embryos, sperm analysis, vitrification and devitrification of gametes and embryos, in vitro fertilization (IVF) and ICSI. It also has a small animal surgery to perform surgery on small animals, mainly mice, which is the animal species with which we preferably work. To carry out these techniques, the Assisted Reproduction Unit is equipped with two intracytoplasmic micromanipulation equipment with IMSI, Laser and Oosight systems, embryo biopsy systems, and mitotic spindle vision systems. We also have a piezoelectric device to carry out ICSI with oocytes from different animal species. We have recently incorporated a Time Lapse incubator to evaluate embryonic development; we also have flow cabinets with stereomicroscopes and heaters, plates, incubators with different gas systems, freezing and cryopreservation equipment for gametes and embryos, among others.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out quality control for Human Reproduction clinics, studies of in vitro fertilization and viability of embryo culture media, Micromanipulation and microinjection techniques of mouse embryos or other animal species, whether that we have piezo electric. Furthermore, as we have a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey.
  Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
  Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
  Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
  https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
  Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
  Methods X. vol 7, pp:1-9; 2020, 100767
  https://doi.org/10.1016/j.mex.2019.12.008

U23-S08. Oosight system

Oosight system

In addition to having micromanipulation and microinjection equipment that allows us to inject sperm into the cytoplasmic cavity of the oocyte (ICSI). One of these microinjectors has a built-in Oosight system implemented which allows us to non-invasively (without adding dyes) precisely observe the location of the mitotic spindle indicative of the correct development and optimal degree of maturation of the oocyte. This facilitates the selection of the most suitable oocyte for the ICSI or IMSI procedure. In addition, Oosight IS indicates the insertion site of the sperm inside the oocyte so as not to damage sensitive cytoplasmic structures.

Customer benefits

Having these micromanipulation and microinjection equipment that incorporates the Oosight system to evaluate the quality and degree of maturation of the oocytes allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out carry out this Technique in the human clinic. Furthermore, the implementation of this oosight imaging system allows them to become familiar with the internal structures of the oocyte and classify it according to its level of development, learning to differentiate which are the most optimal oocytes to later perform ICSI or IMSI. This equipment and the oosight system
It allows the practical training of students of master’s degrees and specialization courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out on animal species, our laboratory is suitable for it. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance and service to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process. without damaging the oocyte. In addition, they can carry out studies that allow visualization of the internal structures and location of the mitotic spindle, which will allow them to select those oocytes that present the best morphology and are fully mature to be fertilized later.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey. Abstract published in: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio. Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio. Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS. Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075. https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts. Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc Methods X. vol 7, pp:1-9; 2020, 100767 https://doi.org/10.1016/j.mex.2019.12.008

U23-S07. Laser System and Biopsed of blastomeres

Laser System and Biopsed of blastomeres

In addition to having micromanipulation and microinjection equipment that allows us to inject sperm into the cytoplasmic cavity of the oocyte (ICSI). One of these microinjectors has a built-in laser system, which allows, by means of small shots, to make a small hole in the membrane of the embryo and, after absorption with specific needles for this process, obtain two or three cells of the trophectoderm and subsequently send these cells to specialized laboratories that perform preimplantation genetic diagnosis studies, to analyze possible abnormalities of the embryo before its transfer and implantation.

Customer benefits

Having these micromanipulation and microinjection equipment that incorporates a laser system to perform trophectoderm biopsy allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out this technique in the human clinic. Furthermore, the implementation of this ICSI technique and blastomere biopsy, and the handling and use of micromanipulators and microinjectors allows the practical training of master’s students and specialization courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out with animal species, our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process without damaging the oocyte In addition, they can perform a biopsy of the embryo’s blastomeres in order to later perform genetic studies on it.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey.
  Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
  Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
  Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
  https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
  Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
  Methods X. vol 7, pp:1-9; 2020, 100767
  https://doi.org/10.1016/j.mex.2019.12.008

U23-S06. IMSI System

IMSI System

Within the Assisted Reproduction Unit we have a laboratory equipped with two micromanipulation and microinjection equipment that allows us to inject the sperm into the cytoplasmic cavity of the oocyte (ICSI), in order to carry out fertilization. The advantage that this equipment offers is that it allows us to perform in vitro fertilization without having to have a large number of sperm and oocytes. Furthermore, to be able to perform this technique with oocytes from different animal species, we have a piezoelectric device to perform this intracytoplasmic injection without damaging the oocyte of the specific animal species. Another possibility offered by this service is that in one of these microscopes we have a 6000x objective, which allows us to see the sperm in large sizes. In this way we can select the sperm that are morphologically of the best quality for intracytoplasmic injection (IMSI), which will subsequently allow us to obtain better quality embryos.

Customer benefits

Having these micromanipulation and microinjection equipment allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out this technique in the human clinic. In addition, the implementation of this ICSI and IMSI technique and the handling and use of micromanipulators and microinjectors allows for practical training for master’s students and specialized courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out with animal species, so our laboratory is suitable for it. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process without damaging the oocyte.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey.
  Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
  Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
  Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
  https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
  Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
  Methods X. vol 7, pp:1-9; 2020, 100767
  https://doi.org/10.1016/j.mex.2019.12.008

U23-S05. In vitro maturation

In vitro maturation

In the assisted reproduction unit we work with experimental animal models, mainly mice, in which we usually extract gametes and embryos, in which we evaluate the maturation process and viability of these oocytes, through tests with different culture media and with enrichment of these culture media with different compounds, to observe which of them produces the best degree of maturation, development and viability of the same, not only of oocytes but also of follicles from other animal species such as sheep. This development and duration can be observed directly using the time-lapse incubator and with different viability tests that are carried out later.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out studies of in vitro fertilization and viability of embryonic culture media for mouse embryos or other animal species, and the development and degree of maturation of oocytes and follicles after the addition of different compounds. to these culture media. Furthermore, by having a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey.
  Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
  Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
  Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
  https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
  Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
  Methods X. vol 7, pp:1-9; 2020, 100767
  https://doi.org/10.1016/j.mex.2019.12.008

U23-S04. Sperm, oocyte and embryo cryopreservation

Sperm, oocyte and embryo cryopreservation

The assisted reproduction unit has a laboratory with extensive equipment with which to perform basic assisted reproduction techniques, including the extraction of gametes (egg and sperm), the extraction of embryos, the analysis of sperm, the vitrification and devitrification of gametes and embryos obtained by in vitro fertilization, IVF and ICSI. Because we work mainly with a murine animal model, obtaining gametes, both oocytes and spermatozoa, is easy and allows us to obtain a large number of gametes, since we have this animal species in the animal facility service of the centre itself, making it easy to access, to this animal model, as well as the large number of gametes that we obtain allows us to create a germplasm biobank, in addition to embryos, which we vitrify and cryopreserve and that can be used and requested by companies for their studies.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out studies of in vitro fertilization and viability of embryo culture media. Furthermore, as we have a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey.
  Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
  Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
  Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
  https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
  Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
  Methods X. vol 7, pp:1-9; 2020, 100767
  https://doi.org/10.1016/j.mex.2019.12.008

U23-S03. Micromanipulation and microinjection

Micromanipulation and microinjection

Within the Assisted Reproduction Unit we have a laboratory equipped with two micromanipulation and microinjection equipment that allows us to inject the sperm into the cytoplasmic cavity of the oocyte (ICSI), in order to carry out fertilization. The advantage that this equipment offers is that it allows us to carry out in vitro fertilization without having to have a large number of sperm and oocytes. Furthermore, to be able to carry out this technique with oocytes from different animal species, we have a piezoelectric device to perform this intracytoplasmic injection without damaging the oocyte of the specific animal species.

Customer benefits

Having these micromanipulation and microinjection equipment allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out this technique in the human clinic. In addition, the implementation of this ICSI technique and the handling and use of micromanipulators and microinjectors allows for practical training for master’s students and specialized courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out with animal species, so our laboratory is suitable for it. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process without damaging the oocyte.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey.
  Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
  Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
  Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
  https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
  Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
  Methods X. vol 7, pp:1-9; 2020, 100767
  https://doi.org/10.1016/j.mex.2019.12.008

U23-S02. Embryo culture media viability studies

Embryo culture media viability studies

Through this service, and through the time-lapse incubator that we have, embryonic development studies can be carried out by enriching the culture media with different compounds or additives to later verify which of them produces better embryonic development and better quality of the embryos, through viability and embryokinetic studies.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.

In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out quality control for Human Reproduction clinics, studies of in vitro fertilization and viability of embryo culture media, of mouse embryos or other animal species, whether that we have piezo electric. Furthermore, as we have a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey.
  Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
  Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
  Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
  https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
  Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
  Methods X. vol 7, pp:1-9; 2020, 100767
  https://doi.org/10.1016/j.mex.2019.12.008

U23-S01. In vitro fertilisation studies and embryo culture media viability

In vitro fertilisation studies and embryo culture media viability

Through this service, in vitro fertilization processes can be carried out, including IVF and ICSI, of oocytes and sperm from different animal species, including mice, which are the animal species we mainly work with. To carry out this fertilization we have cabins, heated laminar flow, with stereomicroscopes included, plates, incubators with different gas systems and in order to evaluate the development of the embryos in real time we have recently incorporated a time lapse incubator, which allows us to carry out embryokinetic studies without having to remove the plates and the embryo from the incubator which improves the quality and viability of the embryo.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out quality control for Human Reproduction clinics, studies of in vitro fertilization and viability of embryo culture media, of mouse embryos or other animal species, whether that we have piezo electric. Furthermore, as we have a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

• Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
  British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
• Optimizations of an ovum pick up protocol in Donkey.
  Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
  https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
• Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
• Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
  Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
  Reproduction. Fertility and Development Sep;34(15):980-990
  https://doi.org/10.1071/RD22041
• 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
  Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
  https://doi.org/10.1093/molehr/gaaa075
• Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
  Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
  Methods X. vol 7, pp:1-9; 2020, 100767
  https://doi.org/10.1016/j.mex.2019.12.008