Fluorescence microscopy
Conventional widefield fluorescence microscopy is still the best choice for many applications. The CCD and CMOS-based sensors used for conventional microscopy are typically much more sensitive than the photomultiplier tubes used in confocal microscopes and flow cytometers. As the camera captures the whole field of view at the same time, it also allows for faster imaging in many cases. Examples where conventional microscopy may be advantageous include the visualization of individual molecules, receptors, or small organisms such as bacteria and yeast.
Customer benefits
We have SOPs and ISO9001 certification. We also have specialist technicians for the use of the equipment.
This service is essential to:
- Nanoscale imaging of fixed samples
- Dynamic imaging of cytoskeletal structures, focal adhesion formation, as well as endocytosis and vesicle dynamics in live cells.
- Fluorescent analysis of histological samples.
Target customer
Any company or research group interested in:
- Long-term (>4 days) imaging of bacterial colonies and bacterial interactions using DIC, temperature control, autofocus, and multi-field imaging.
- Routine multi-color fluorescent analysis of histological samples where confocal or other more advanced methods are not necessary.
- Imaging of bone, muscle, or connective tissues in histological samples using circularly-polarised light.
References
Carrillo P, Bernal M, Téllez-Quijorna C, Marrero AD, Vidal I, Castilla L, Caro C, Domínguez A, García-Martín ML, Quesada AR, Medina MA, Martínez-Poveda B. The synthetic molecule stauprimide impairs cell growth and migration in triple-negative breast cancer. Biomed Pharmacother. 2023 Feb;158:114070. doi: 10.1016/j.biopha.2022.114070. Epub 2022 Dec 14. PMID: 36526536.