Freeze substitution
Freeze Substitution (FS) of specimens in methanol, acetone or any other FS media at low temperatures is the next step following high pressure freezing and other cryo-fixation methods.
Progressive Lowering of Temperature (PLT) allows substitution and resin infiltration of chemically fixed specimens. Finally, the sample is polymerized with epoxy resins for conventional electron microscopy or tomography analysis, or with acrylic resins under UV light for immunolabelling studies.
Customer benefits
We have SOPs and ISO9001 certification. We also have specialist technicians for the use of the equipment.
Fundamental research in cellular and structural biology for electron tomography and immulabelling studies at room temperature, avoiding cryo-electron microscopy drawbacks.
Target customer
Any company or research group interested in:
- Structrural biology.
- Cell and tissue samples processed after high pressure or not suitable for high temperature resin embedding.