High-pressure freezing
High pressure freezing is the most significant sample preparation method for morphological and immunocytochemical high resolution studies for electron microscopy.
High pressure freezing has made it possible to observe aqueous biological and industrial samples near to native state.
The 2100 bar of high pressure applied to the sample during high pressure freezing using the Leica EM HPM100 suppresses ice crystal formation and growth, while cryo-immobilization immediately after pressurization prevents structural damage to the sample.
High pressure frozen samples can be completely vitrified up to a thickness of 200 µm, a 10 to 40-fold increase in the depth of amorphous ice.
Customer benefits
We have SOPs and ISO9001 certification. We also have specialist technicians for the use of the equipment.
Cellular ultrastructure visualization using electron tomography to obtain a 3D reconstruction of cells and their internal structures close to a native state, improving the information provided by standard 2D analysis.
Target customer
Any company or research group interested in:
- Structural biology.
- Cell and tissue samples not suitable for chemical fixation.