Laser System and Biopsed of blastomeres
In addition to having micromanipulation and microinjection equipment that allows us to inject sperm into the cytoplasmic cavity of the oocyte (ICSI). One of these microinjectors has a built-in laser system, which allows, by means of small shots, to make a small hole in the membrane of the embryo and, after absorption with specific needles for this process, obtain two or three cells of the trophectoderm and subsequently send these cells to specialized laboratories that perform preimplantation genetic diagnosis studies, to analyze possible abnormalities of the embryo before its transfer and implantation.
Customer benefits
Having these micromanipulation and microinjection equipment that incorporates a laser system to perform trophectoderm biopsy allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out this technique in the human clinic. Furthermore, the implementation of this ICSI technique and blastomere biopsy, and the handling and use of micromanipulators and microinjectors allows the practical training of master’s students and specialization courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out with animal species, our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.
Target customer
This service can provide assistance to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process without damaging the oocyte In addition, they can perform a biopsy of the embryo’s blastomeres in order to later perform genetic studies on it.
Additional information
- Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033 - Optimizations of an ovum pick up protocol in Donkey.
Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4 - Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060 - Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
Reproduction. Fertility and Development Sep;34(15):980-990
https://doi.org/10.1071/RD22041 - 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
https://doi.org/10.1093/molehr/gaaa075 - Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
Methods X. vol 7, pp:1-9; 2020, 100767
https://doi.org/10.1016/j.mex.2019.12.008