U26. NMR: Biomedical Applications II – Equipment

U26-E07. MRS*DRYMAG3017/MRS*PET CLIP-ON MR SOLUTIONS

Equipment Features:

Description: 3T preclinical imaging equipment with PET ClipOn attached (MRS*CT Benchtop – MR SOLUTIONS).

Technical specifications: Imaging equipment with superconducting magnet, capable of varying the angle from 0 to 90º, without cryogenics of 3 Teslas; mouth diameter of 170mm; 600 mT/m gradients with 5% linearity; 30 mm DSV for Homogeneity and 0.05 ppm/hour for stability; 350 Kg of total weight.

Applications:

Acquisition and viewing of in vivo images of mice and rats as well as other beings or materials of similar size.

• Qualitative and quantitative studies (relaxation times T1 and T2 and even making maps) at the brain level and other organs.
• Cardiological sequences: diffusion, determination of blood volume…
  Anatomical and functional studies of different organs (for example: renal, abdominal, liver…).
• Bone-level imaging, distinguish between bone material from soft tissue.
  Fat suppression techniques.
• Oncological studies to detect and locate tumors and volume calculation.
• Carry out studies on fruits and vegetables (grapes, kiwi, tomato, seeds…).
• Perform studies with/without contrast (Gadolinium) and see the differences between the 2 images.
• Thanks to the possibility of rotating the equipment in a vertical position, images of plants can be obtained (because by their nature, they have to be in a vertical orientation, such as a stem).
• Ex-vivo studies can also be carried out vertically and in this way we maintain the integrity of the organ (eg heart).

For more information on how we use this equipment, visit here.

U26-E06. Gilson 215 liquid handler for automated sample preparation of liquid samples

U26-E02. -NMR microscopy system for imaging Bruker 14T

• Functional and metabolic studies in cells, tissues and animal models. Coils 2mm, 5mm and 10mm. Image of 1H, 13C and 31P.
• Microcamera for its use in the NM R equipment for:
• The simultaneous determination of structural images (dimensions and sections of 50 μm and less)
• Metabolic images (voxel sizes < 200 μm) in model systems.

See this equipment for more information on how this device is used.

U26-E05 Bruker Avance III 400 WB spectrometer, with ultrashielded widemouth magnet (89 mm), 400 MHz (9.4 T), with two RF channels and high power amplifiers for solid-state experiments.

Accessories:

• DVT 1H/BB Probe. 4mm multinuclear probe, with 1H channel and broadband channel with range frequency from 15N to 31P.
• DVT 1H/BB Probe. 7mm multinuclear probe, with 1H channel and broadband channel with range frequency from 15N to 31P.
• 2H lock channel for observing liquids.
• PA BBOF Probe. 5mm direct multinuclear probe with broadband channel (range frequency from 15N to 31P) and additional observation of 19F.
• Microprobe MicWB40 for imaging a wide range of small ex vivo samples up to 25 mm

U26-E03. Bruker Neo500 spectrometer (11.7 T), and with three RF channels

11T NMR equipment with a multinuclear probe with service for metabolomics for tissues, cell lines and biofluids.

Accessories:

• BBOF Plus ATM. 1H/BB/19F direct multinuclear probe (5mm), with field gradients along the z axis, and automatic frequency tuning. Range of frequencies in the broadband channel from 15N to 31P.
• TBI. 1H/BB/15N triple reverse probe (5mm), with field gradients along the z axis. Range of frequencies in the broadband channel from 15N to 31P.
• SampleCase of 24 samples.
• Accessory to control temperature, with air down to 5ºC. Liquid nitrogen evaporator for low temperature experiments.

U26-E01. DRX-600 Bruker NM R equipment (14 Teslas)

DRX-600 Bruker NM R equipment (14 Teslas):

• 4-channel electronic systems with applications in fluids, tissues, biomaterials and microimaging
• Thermostating system for fluids and tissues, and for molecular imaging and microimaging
• Set of coils: fluids 5 mm, micro-fluids 1 mm, tissues (High Resolution Magic Angle Spinning, HR-MAS) + pneumatic and microimaging system for 10, 5 and 2 mm.
• 60 Amp GREAT unit for each X/Y/Z axis of the field gradients
• 13C selective device with 1H decoupling.
• 2D microcoil for 500 μm and 100 μm microsamples.
• Refrigerated SampleJet autosampler with temperature control for 60 Samples

To learn more about this equipment, visit the website of the Universidad de Valencia.