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U2-S01. Scientific and technical support

Scientific and technical support

The scientific and technical support service provides valuable assistance to researchers and organizations in various aspects:

  • Immunogen Design: Expert advice on designing effective immunogens for antibody production.
  • ­Immunoreagent Synthesis and Production: Guidance on synthesizing and producing immunoreagents, including monoclonal and polyclonal antibodies.
  • Antibody Production: Support for generating high-quality antibodies for research purposes.
  • Immunoassay Design and Development: Customized development of immunoassays, including assay format design, sample matrix considerations, and sensitivity optimization.

The technical support provided is focused on identifying suitable immunogens for antibody production with desired characteristics for the user, while in the antibody production section, support is oriented towards designing screening methods during monoclonal antibody development to search for hybridomas with the desired specificity and sensitivity characteristics for each user.

Customer benefits

  • Scientific Expertise: Access to specialized knowledge in immunology and immunochemistry.
  • Technical Guidance: Assistance in experimental design, troubleshooting and optimization.
  • Cost-Effective Solutions: Avoiding the need to establish in-house facilities for antibody production and immunoassay development.
  • Accelerated Research: Faster progress due to expert support and streamlined processes.
  • ISO 9001 Certification: The development and production of monoclonal antibodies is backed by the ISO 9001:2015 certification, ensuring quality, reliability and adherence to international standards.

Target customer

Scientific and Technical Support Serviceis essential for organizations involved in Research and Development (R&D) across fields such as food safety and environmental control,  biomedicine, diagnostics, drug discovery, and therapeutic development.

Additional information

Selected references:

  • B. Rodriguez-Urretavizcaya, N. Pascual, C. Pastells, M. T. Martin-Gomez, Ll. Vilaplana, M.-P. Marco. Diagnostic and Stratification of Pseudomonas aeruginosa Infected Patients by Immunochemical Quantitative Determination of Pyocyanin from Clinical Bacterial Isolates. Frontiers in Cell. Infect. Microbiol., 11, 786929, 2021. DOI: 10.3389/fcimb.2021.786929. URL
  • Giovanna Roncador; Pablo Engel; Lorena Maestre; et al; Alison H.Banham., Nuria Pascual 2016. The European antibody network’s practical guide to finding and validating suitable antibodies for research. mAbs. Taylor & Francis Online. 8-1, pp.27-36.
  • Carme Pastells; Gerardo Acosta; Nuria Pascual; Fernando Albericio, Miriam Royo; M.-Pilar Marco. 2015. An immunochemical strategy based on peptidoglycan synthetic peptide epitopes to diagnose Staphylococcus aureus infections. Analytica Chimica Acta. Elsevier. 889, pp.203-211
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U6-S14. Quantitative studies of biomolecular interactions by calorimetric measurements (On-site) OUTSTANDING

Quantitative studies of biomolecular interactions by calorimetric measurements

Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event (protein-small molecule, protein-protein, target-drug, enzyme-inhibitor, antibody-antigen, protein-DNA, protein-lipid, small molecule-small molecule). Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment. Because ITC goes beyond binding affinities and can elucidate the mechanism of the molecular interaction, it has become the method of choice for characterizing biomolecular interactions.
The equipment used for this purpose is VP-ITC (GE HealthCare-Microcal).

Customer benefits

Applications range goes from drug design to fundamental research, such as understanding and regulating signal transduction pathways. These systems provide direct marker-free and in-solution measurement of binding affinity and thermodynamic parameters in a single experiment. They have high sensitivity, low sample consumption and automation options to minimise handling time.

Target customer

  • Biochemical and Pharmaceutical companies.
  • Biology and biochemistry research groups.

Additional information

M.Köber, et al., Journal of Colloid and Interface Science 631 (2023) 202–211. DOI: 10.1016/j.jcis.2022.10.104

Compra al mejor precio MALVERN MicroCal VP-ITC | Bimedis

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U1-S03. Proteins purification

Proteins purification

The PPP Unit counts with a laboratory perfectly equipped for the purification of recombinant proteins. The protein purification procedure is carried out according the protein characteristics and customers‘ needs.
Design: identification of the most appropriate strategy for protein purification, given the physical and chemical properties, opting either for purification modules or, in the case of fusion proteins, elements which enable purification by affinity.
Protein purification: downstream processing using FPLC chromatography and/or tangential filtration either from the cellular pellet or the supernatant when the protein is secreted. When necessary, the purification is performed through solubilization of inclusion bodies or by purification under denaturing conditions with later refolding.
Final product: the purified protein is delivered in the buffer, temperature and the concentration requested by the costumer. When demanded, the protein can be delivered in lyophilised form.
Quality control: the purified recombinant protein is analysed in terms of molecular weight, purity by SDS-PAGE, aggregation by DLS and identification by Peptide-Mass Fingerprinting. Additional analysis can be performed under customer’s agreement such as buffer optimization (Microdyalisis) or freeze-thaw stability.

Customer benefits

The service is completely personalised to ensure that the final product matches the intended use in terms of format, purity and activity. We have various purification methodologies to address specific needs, offering a wide range of possibilities for obtaining the final product. All services adhere to standardised procedures, guaranteeing the quality of the end product. Thus, instead of offering a generic solution, we deliver a customised product with the exact specifications requested by the customer.

Target customer

The PPP Unit extends its services across the scientific community, serving both private and public research organizations. This includes support for research centres, universities, hospitals, and companies in the sector. Leveraging our connection with the university, the PPP unit also provides specialised courses as part of the official master’s degrees and PhD programs at UAB.

Additional information

Over its 17-year history, the PPP unit has successfully completed over 400 projects and has been recognised in more than 121 scientific publications. In the past three years, it has received citations in the following works:

  • ACS Materials Lett. 2024, 6, 3, 954–962
  • Front. Immunol. 2024. 15:1346512.
  • Applied Microbiology and Biotechnology (2024) 108:98
  • ACS Sustainable Chem. Eng. 2023, 11, 4133−4144
  • Pharmaceutics 2023, 15, 2632.
  • Microbial Cell Factories (2023) 22:81
  • Biomed Pharmacother 2023. 164:114976.
  • ACS Appl. Mater. Interfaces 2023, 15, 39167−39175
  • Pharmaceutics 2023, 15, 1197
  • Sci China Mater 2023, 66(10): 4109–4120
  • Pharmaceutics 2023, 15(3), 727
  • Biomedicine & Pharmacotherapy 2023, 169(1):115848
  • Appl Environ Microbiol 2023 Jun 28;89(6)
  • Biomedicine & Pharmacotherapy 150 (2022) 112940
  • Biomaterials 280 (2022) 121258
  • Pharmaceutics 2022, 14, 887
  • J. Exp Clin Cancer Res (2022) 41:49
  • DRUG DELIVERY 2022, VOL. 29, NO. 1, 1384–1397
  • Biomedicines 2022, 10, 1680.
  • Microbial Cell Factories (2022) 21:203
  • Acta Pharmaceutica Sinica B 2022;12(5):2578e2591
  • Pharmaceutics 2022, 14, 602.
  • Pharmaceutics 2022, 14, 192.
  • Front. Bioeng. Biotechnol. 2022 10:842256.
  • Anal. Chem. 2022, 94, 5359−5366
  • Odontology (2022) 110:545–556
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U1-S02. Bioproduction of proteins

Bioproduction of proteins

The PPP Unit counts with a laboratory perfectly equipped for the bioproduction of recombinant proteins. The expression system is selected according to the protein characteristics and customer’s needs.
Transformation/transfection: the engineered construct is introduced inside the chosen expressing cells. In certain instances, involving insect expression, this step includes the isolation of a bacmid made for protein production via the baculovirus system.
Optimisation: selection of the optimal protein production conditions using small and medium-scale expression; confirmation of the protein expression using SDS-PAGE and Western blot analysis and quantification by densitometry. At this step protein solubility/aggregation is measured to obtain the best solubilizing conditions.
Recombinant protein production: scale-up of the upstream processing by batch culture according to the best protein production conditions selected during the optimization step.

Customer benefits

The service is completely personalised to ensure that the protein matches the intended use for later protein purification. Thus, instead of offering a generic solution, we deliver a customised product with the exact specifications and needs requested by the customer.
We have various expression systems to address specific needs, offering a wide range of possibilities for obtaining the final product. All services adhere to standardised procedures, guaranteeing the quality of the end product.

Target customer

The PPP Unit extends its services across the scientific community, serving both private and public research organizations. This includes support for research centres, universities, hospitals, and companies in the sector. Leveraging our connection with the university, the PPP unit also provides specialised courses as part of the official master’s degrees and PhD programs at UAB.

Additional information

Over its 17-year history, the PPP unit has successfully completed over 400 projects and has been recognised in more than 121 scientific publications. In the past three years, it has received citations in the following works:

  • ACS Materials Lett. 2024, 6, 3, 954–962
  • Front. Immunol. 2024. 15:1346512.
  • Applied Microbiology and Biotechnology (2024) 108:98
  • ACS Sustainable Chem. Eng. 2023, 11, 4133−4144
  • Pharmaceutics 2023, 15, 2632.
  • Microbial Cell Factories (2023) 22:81
  • Biomed Pharmacother 2023. 164:114976.
  • ACS Appl. Mater. Interfaces 2023, 15, 39167−39175
  • Pharmaceutics 2023, 15, 1197
  • Sci China Mater 2023, 66(10): 4109–4120
  • Pharmaceutics 2023, 15(3), 727
  • Biomedicine & Pharmacotherapy 2023, 169(1):115848
  • Appl Environ Microbiol 2023 Jun 28;89(6)
  • Biomedicine & Pharmacotherapy 150 (2022) 112940
  • Biomaterials 280 (2022) 121258
  • Pharmaceutics 2022, 14, 887
  • J. Exp Clin Cancer Res (2022) 41:49
  • DRUG DELIVERY 2022, VOL. 29, NO. 1, 1384–1397
  • Biomedicines 2022, 10, 1680.
  • Microbial Cell Factories (2022) 21:203
  • Acta Pharmaceutica Sinica B 2022;12(5):2578e2591
  • Pharmaceutics 2022, 14, 602.
  • Pharmaceutics 2022, 14, 192.
  • Front. Bioeng. Biotechnol. 2022 10:842256.
  • Anal. Chem. 2022, 94, 5359−5366
  • Odontology (2022) 110:545–556
• Read More

U1-S01. Molecular Cloning (Biomolecules production)

Molecular Cloning (Biomolecules production)

Design: depending on the customers‘ needs and protein characteristics (size, modifications, etc.) we identify the most appropriate expression system for recombinant protein production selecting between Escherichia coli, insect cells (with expression systems based on transient expression or by infection with recombinant baculovirus ) and mammalian cells.
Cloning: the recombinant gene for the protein of interest, once optimised for codon usage, is cloned into the selected expression vector. When necessary, affinity purification tags, solubilizing tags and/or secreting signal peptides are incorporated into the design.
Sequencing: all constructs are sequenced to verify the accuracy of the gene sequence.

Customer benefits

The service is completely personalised to ensure that the final construct matches the intended use for later protein production in the chosen expression system and further purification. Thus, instead of offering a generic solution, we deliver a customised product with the exact specifications requested by the customer.

Target customer

This Unit extends its services across the scientific community, serving both private and public research organizations. This includes support for research centres, universities, hospitals, and companies in the sector. Leveraging our connection with the university, the PPP unit also provides specialised courses as part of the official master’s degrees and PhD programs at UAB.

Additional information

Over its 17-year history, this unit has successfully completed over 400 projects and has been recognised in more than 121 scientific publications. In the past three years, it has received citations in the following works:

  • ACS Materials Lett. 2024, 6, 3, 954–962
  • Front. Immunol. 2024. 15:1346512.
  • Applied Microbiology and Biotechnology (2024) 108:98
  • ACS Sustainable Chem. Eng. 2023, 11, 4133−4144
  • Pharmaceutics 2023, 15, 2632.
  • Microbial Cell Factories (2023) 22:81
  • Biomed Pharmacother 2023. 164:114976.
  • ACS Appl. Mater. Interfaces 2023, 15, 39167−39175
  • Pharmaceutics 2023, 15, 1197
  • Sci China Mater 2023, 66(10): 4109–4120
  • Pharmaceutics 2023, 15(3), 727
  • Biomedicine & Pharmacotherapy 2023, 169(1):115848
  • Appl Environ Microbiol 2023 Jun 28;89(6)
  • Biomedicine & Pharmacotherapy 150 (2022) 112940
  • Biomaterials 280 (2022) 121258
  • Pharmaceutics 2022, 14, 887
  • J. Exp Clin Cancer Res (2022) 41:49
  • DRUG DELIVERY 2022, VOL. 29, NO. 1, 1384–1397
  • Biomedicines 2022, 10, 1680.
  • Microbial Cell Factories (2022) 21:203
  • Acta Pharmaceutica Sinica B 2022;12(5):2578e2591
  • Pharmaceutics 2022, 14, 602.
  • Pharmaceutics 2022, 14, 192.
  • Front. Bioeng. Biotechnol. 2022 10:842256.
  • Anal. Chem. 2022, 94, 5359−5366
  • Odontology (2022) 110:545–556
• Read More