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U30-S05. Targeted proteomics analysis by MRM or PRM-MS

Targeted proteomics analysis by MRM or PRM-MS

Relative or absolute quantification of individual proteins or protein panels by the analysis of their peptides together with stable isotope labelled (SIL) standards.

Customer benefits

This strategy is employed to verify or validate biological hypotheses with the highest specificity, as it detects the protein sequence and is not dependent on antibodies. The use of isotope labelled standards allows the absolute quantification of the protein/s of interest.

Target customer

Organizations and research groups interested in the analysis and quantification of a protein or panel of proteins with high specificity.

U30-S05 Targeted proteomics analysis by MRM or PRM-MS
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U30-S04. Quantitative shotgun proteomics by nLC-MS/MS

Quantitative shotgun proteomics by nLC-MS/MS

Large-scale relative quantification of proteins in biological samples by nano-flow high performance liquid chromatography combined with mass spectrometry. It can use either data-dependent (DDA) or data-independent (DIA) acquisition modes. Quantifications can be performed via label-free (LFQ) or protein/peptide isotopic labelling using SILAC, iTRAQ or TMT reagents.

Customer benefits

The output of this analyses are lists of proteins identified in the samples, together with quantitative information obtained from the area displayed by each peptide in the mass spectra, and can then be compared across experimental conditions.

Target customer

Organizations and research groups interested in biomarker discovery, drug discovery (protein target identification), development of precision medicine approaches, etc.

U30-S04 Quantitative shotgun proteomics by nLC-MS/MS
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U30-S03. Protein identification by mass spectrometry (MS) or MS/MS

Protein identification by mass spectrometry (MS) or MS/MS

Identification of proteins in biological samples, either by intact protein analysis (MALDI-TOF) or protein digestion followed by peptide mass fingerprinting (PMF, MS) or peptide fragmentation (MS/MS).

Customer benefits

Identification of proteins by mass spectrometry allows the characterization of biological samples, analysis of protein interactions (e.g. pull-down assays), peptide sequencing, etc. Identifications are performed with the highest specificity.

Target customer

Organizations and research groups interested in small or large-scale protein analysis.

Additional information

E03 MALDI-TOF/TOF System

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U30-S02. Determination of molecular mass of peptides, proteins and other biomolecules

Determination of molecular mass of peptides, proteins and other biomolecules

Analysis of intact proteins, peptides and other biomolecules such as oligonucleotides using mass spectrometry (MALDI-TOF or ESI-Q-TOF) for the determination of their exact molecular mass.

Customer benefits

This service is useful to characterize a variety of biomolecules by the determination of their molecular mass with an extreme high precision. This is an essential step in many biotechnological strategies involving protein synthesis and production.

Target customer

Organizations or research groups aiming to characterize specific proteins, peptides or other biomolecules by the determination of their molecular mass.

U30 S02 Determination of molecular mass of peptides, proteins and other biomolecules
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U30-S01. Sample preparation for proteomic analysis

Sample preparation for proteomic analysis

This service includes all necessary steps to prepare samples for different proteomic analyses: protein extraction from tissues, cells or fluids, precipitation, cleaning, desalting, quantification and digestion (in gel or in solution) with proteolytic enzimes (trypsin, chymotrypsin, lys-C…), to obtain the correspondent peptides.

Customer benefits

Biological samples are processed and prepared following standardized protocols by personnel highly specialized in techniques for protein analysis.
The service allows organizations without prior experience in protein or proteomic analysis to avoid performing any experiment, only needing to provide the biological specimen to the Unit.

Target customer

Organizations or research groups interested in performing protein or proteomic analyses in a wide range of biological samples: cells, tissues, biofluids, protein extracts, etc.

U30 S01 Sample preparation for proteomic analysis
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U26-S06. Biomedical and metabolomic imaging

Biomedical and metabolomic imaging

The service is intended for obtaining images animals such as mice and rats. Longitudinal studies can be performed as well. Thanks to a MRS*DRYMAG 3.0T with MRS*PET CLIP-ON (a PET plug-in), co-localized NMR-PET-CT imagens can be obtained.

Customer benefits

The service is integrated in the University of Valencia core facility that ensures the correct maintenance and the offsite service runed by technicians under ISO 9001.

Target customer

The primary audience are pre-clinic research groups that require punctual or longitudinal studies on such animals. Plant and food researchers can benefit from the fact that the equipment can acquire the image in vertical position preserving the natural flux of fluids in plants.

Additional information

Root growth of a maize plant in soil monitored over several days with MRI. Imaged published in https://doi.org/10.1104/pp.15.01388 under Creative Commons.
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U8-S05. Deposition of thin films (Polyimide, Parylene) for the fabrication of flexible devices or encapsulation purposes (On-site&Remote) OUTSTANDING.

Deposition of thin films (Polyimide, Parylene) for the fabrication of flexible devices or encapsulation purposes (On-site&Remote) OUTSTANDING.

Deposition of Polyimide (PI-2611) via spin-coating for the fabrication of flexible free-standing structures.
Conformal deposition of Parylene (different types available)

Customer benefits

  • Possibility to access to the PI deposition system in self-service mode to adapt the process to the customer needs.
  • Different Parylene types available for deposition to match customer needs

Target customer

Research groups and SMEs

References

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U8-S06. Encapsulation or fabrication of 3D strcutures via 3D printing.

Encapsulation or fabrication of 3D strcutures via 3D printing.

Fabrication of 3D structures using a dual extrusion 3D printer that delivers high-quality, composite-ready performance – in a smaller footprint

Customer benefits

Possibility to access to the 3D printing equipment in self-service mode to adapt the process to the customer needs.

Target customer

Research groups and SMEs

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U20-S015. Cytotoxicity: proliferation, LDH, apoptosis and ROS

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U20-S014. Hemocompatibility: hemolysis, platelet aggregation, plasma coagulation times and complement activation

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