Preclinical validation characterization - Equipment - Nanbiosis

U15-E01. Alternating Gradient Magnetometer

Alternating Gradient Magnetometer:

MicroMag M2900-4 AGM (Princeton Measurements Corporation, USA) for the magnetic and mechanical characterization of nanoparticles in various different media:
•• Magnetic Moment Range: 1nA.m² to 5mA.m² full scale (1μemu to 5emu).
•• Resolution: 0,005% of full scale with 60% overrange capability.
•• Speed of measurement: 100 ms/point.
•• High sensitivity: 10 pA.m² (10 nemu) of standard deviation at room temperature and with 1 second of averaging time.
•• High performance with samples of very small magnetization and dimensions (few nanometers).
•• The AGM can accommodate a large range of samples with widely different properties.

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U14-E01. Cell culture rooms

Cell Culture Room 1:
MSC Advantage Class II b Biological Safety Cabinet (Thermo)
CO2 incubator Heracell 150 (Thermo)
Nikon Eclipse TE2000 fluorescence inverted microscope (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)

Cell Culture Room 2:
2 MSC Advantage Class II b Biological Safety Cabinets (Thermo)
2 CO2 incubators Heracell 150 (Thermo)
Phases inverted microscope (NIKON)
2 Vacuum Pumps ME4 (Vacuubrand)
Cell Counter (Invitrogen)

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U14-E02. Molecular biology

MACSQuant 10 Analizer Flow Cytometer (Miltenyi Biotec)

Microplate reader Synergy MX (BIOTEK)

QuantStudio 3 Real-Time PCR System (AppliedBiosistem)

UV/Vis Spectrophotometer (IMPLEN)

Thermal Cycler PXE 0.2 (THERMO)

Fume Hood Nublar NBL-1600 (Formimetal)

Refrigerated centrifugeMultifuge 1 (Heraeus)

Refrigerated centrifuge for microtubes Heraeus Fresco 21 (Thermo)

Rotary shaker with incubation (HEIDOLPH)

Digital circulation thermostatic bath (GRANT)

Manual variable volume pipettes (Eppendorf, Kartell, Rainin)

Multichannel variable volume pipettes (Thermo)

Pipette controllers (Gosselin, Rainin, Lab box)

Liquid Nitrogen tank (Taylor Wharton)

Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80°C) (HERAEUS)

Freezers -20°C (FIOCHETTI, Indesic)

Refrigerators +2/+8ºC (Thermo, CLASS ELECTRONIC)

Thermoblock Accublock (Labnet)

Heating magnetic stirrer ARE (VELP Scientifica)

Analytical balance (Metria)

Precision analytical balance Pioneer (OHAUS)

pH-Meter Symphony (VWR)

Ultrasonic water bath (SELECTA)

Vortex 230 V, EU (VWR)

Microwave (LG)

Crushed ice machine (Schottman)

Autoclave Presoclave-II (P SELECTA)

Sterilization incubator (Modelo UT ) (HERAEUS)

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U13-E02. Machines for mechanical tests

Machines for mechanical tests:

• Instron MicroTester 5848 and 5548: electromechanical machines for displacement or load control tests:

•• load range from 1mN to 2kN at full scale.
•• load cells of:+/- 5, 50, 500 and 1000N.
•• synchronous data capture in all the channels up to
500 Hz.
• Biaxial Instron MicroTester 8874: axial-torsional, servohydraulic testing machine:
•• load capacity of 25 kN and 100 Nm.
•• fatigue tests at a low number of cycles.

›› Software for data control and analysis.
›› Accessories and tools especially designed by the group, which allow a wide flexibility in designing tests, allowing the verification and/or validation of theoretical models.
›› AVE video extensometer connected to the machines; resolution of 0.5 μm. To determine the axial and transverse deformation without contact (tension, dynamic properties and viscoelasticity) of the samples.

• E1000 Test Instrument: Dynamic systems to offer slow-speed static testing and high-frequency dynamic fatigue testing with hundreds of Hertz capability: Load range: 250 N to 2.0 KN.
• Nano BIONIX MTS: For nanomechanical characterization of biomaterials. Load range to 0.001N to 0.5N.

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U13-E01. MicroCT GE eXplore Locus SP (GE)

MicroCT GE eXplore Locus SP (GE): Computed X-ray Axial Tomograph for the non-destructive study of the microstructure and in vitro tomography: ›› Samples of up to 40 mm in diameter; resolution of 10 μm. ›› Different types of tissues, implants, scaffolds, etc. ›› Complete “section-by-section” imaging with a single rotation (cone beam volumetric technology); excellent image quality and “signal-to-noise” ratio. ›› “Bone Analysis Tool” software provided with: •• BMD (Bone Mineral Density): It calculates the density of minerals in the bone, the bone volume fraction and the mineral content in the bone. •• Anisotropy: It determines the degree of symmetry and the orientation of the trabecular structure. •• Cortical Analysis: It determines “section-by-section” the width, surface area and the BMD value of the cortical bone. •• Stereology: It provides the bone volume fraction, the ratio between the surface of the bone and its volume and the width of the trabecular plate. •• Direct Measures: It determines the local trabecular width of a bone and allows obtaining a visual representation of this local width.

Temporarily OUT OF ORDER

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U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

Description:
High-speed centrifuge with aluminum rotors at fixed angle – Rotor JA-25.15 (maximum centrifugal force = 74200g, maximum speed = 25000 rpm, capacity for 24 tubes of 15 mL each, total capacity = 360 mL) – Rotor JLA-16.250 (maximum centrifugal force = 38500g, maximum speed = 16000 rpm, capacity for 6 tubes of 250 mL each, total capacity = 1500 mL) – Rotor JA-10 (maximum centrifugal force = 17700g, maximum speed = 10000 rpm, capacity for 6 tubes of 500 mL each, total capacity = 3 L)

Applications:
It allows to centrifuge samples such as emulsions, dispersions, etc at angular velocities up to 25000 rpm (74200 g) at various temperatures (from -20˚C a 40˚C), which can be used for phase separation, stability studies, etc. in colloidal systems and for the separation of cells and proteins.

U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

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U12-E13. Turbiscan Lab backscattering stability analyzer (Formulaction)

U12-E13. Turbiscan-lab-backscattering-stability-analyzer-formulaction

Description:
Instrument for the evaluation of colloidal stability. It measures turbidity by multiple light scattering, as a function of time along the vertical axis by both transmitted and backscattered light. It can analyse samples up to 95 vol% with a wide range of particle sizes (10 nm – 1 mm). The samples are placed inside a temperature-controlled chamber, and the measurements can be carried out from room temperature up to 60 ˚C.

Applications:
Allows to study the colloidal stability of emulsions, suspensions and foams and to determine whether there is sedimentation, creaming, flocculation or coalescence.

U12-E13. Turbiscan-lab-backscattering-stability-analyzer-formulaction

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U12-E12. BX51TRF6 Olympus Optical Microscope

Description:
Optical microscope that can work with transmitted or reflected light. Different observation methods can be used: bright field, dark field, phase contrast, differential interference contrast, polarized light, and fluorescence. It includes two revolvers to work at short or long distances. Moreover, it is equipped with a digital camera (Olympus DP73) connected to the computer with a software that captures images and videos.

Applications:

Observation of samples at microscale

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U12-E11. Densitometer DMA 4500M (Anton Paar)

U12-E11. DMA -46 densitometer (Anton Paar)

Description:

Instrument for the Determination of the density of liquid samples using Pulsed Excitation. It is a highly precise instrument, measuring density by the method of resonance vibration frequency of a glass capillary.

Applications:

Measurement of the density of liquid samples at controlled temperature

U12-E11. DMA -46 densitometer (Anton Paar)

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U12-E10. Optilab rEX Differential Refractometer

Description:
The Optilab® rEX (refractometer with EXtended range) is a Refractive Index measurement system. It has a flow cell with a total volume of only 7.4 µL. This translates into minimized band broadening and better temperature stability. It can measure the absolute refractive index of a solution. In addition, it can measure the dn/dc of a solvent at a fixed wavelength. The light source in the rEX can be replaced for other wavelengths (e.g. 690, 633, 488 nm, etc.). The temperature can be programmed from 4°C to 50°C. It is compatible with all HPLC systems.

Applications:
Refractive Index determination, mainly of liquid samples, can be useful in measurements of other techniques (Dynamic Light Scattering as an example). It can be also possible to calculate the refraction index as a function of concentration for a compound. This can allow you to determine the purity or concentration of the compound which is dissolved in a liquid.

$U12-E10. Optilab rEX Differential Refractometer$

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Preclinical validation characterization - Equipment

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