Micromanipulator and microinyection of last generation in conjunction with the most advanced optical microscope IMSI system
11T NMR equipment with the following probes:
•• HR-MAS,
•• Cryoprobe, and
•• multinuclear probe with service for metabolomics for tissues, cell lines and biofluids.
Accessories:
DRX-600 Bruker NM R equipment (14 Teslas):
•• 4-channel electronic systems with applications in fluids, tissues, biomaterials and microimaging
•• Thermostating system for fluids and tissues, and for molecular imaging and microimaging
•• Set of coils: fluids 5 mm, micro-fluids 1 mm, tissues (High Resolution Magic Angle Spinning, HR-MAS) + pneumatic and microimaging system for 10, 5 and 2 mm.
•• 60 Amp GREAT unit for each X/Y/Z axis of the field gradients
•• 13C selective device with 1H decoupling.
•• 2D microcoil for 500 μm and 100 μm microsamples.
•• Refrigerated SampleJet autosampler with temperature control for 60 Samples
Animal housing room and animal preparation area equipped with a vital constants monitoring system, 2 full sets of anesthesia equipment, water bath, infrared lamp, injection pump, etc.
Software packages for data acquisition and processing:
Two AVANCE 250 MH z (5.8 T) spectrometers, one of them with an autosampler.
An AVANCE 360 MH z (8.4 T) spectrometer for the highresolution
analysis of samples in liquid state. Suitable for the structural determination of chemical molecules.
AVII 400 MH z (9.4 T) spectrometer – with a narrow bore, equipped with a solid state accessory for working under Cross Polarization Magic-Angle-Spinning (CPMAS) conditions with materials under solid conditions. It has rotors with a variable capacity between 12-40 μl, 4 mm-diameter and a spinning rate up to 15000 Hz.
AVIII 400 MH z (9.4 T) spectrometer – with a narrow bore, equipped with a High Resolution Magic Angle Spinning (HRMAS) probe for analyzing semi-solid/semiliquid samples such as biopsies, cell extracts or biofluids. It has rotors with a variable capacity between 12-40 μl, 4 mm-diameter and a spinning rate up to 15000 Hz.
AVANCE 500 MH z (11.7T) spectrometer with a 5 mm TCI cryoprobe with a high sensitivity increase (4X). It is connected to a high pressure liquid chromatography (HPLC), mass spectrometry (MS) and Diode Array (DAD) detectors. It has the possibility of using solid-phase extraction (SPE). It is unique for metabolomic studies and analysis of samples with limited mass and for the characterization of complex samples with compounds at a low concentration (μg), for biomedical and pharmacological applications, and analysis of natural products
