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Posters presentation by NANBIOSIS Units in CIBER-BBN ANNUAL CONFERENCE 2017

Last 13 and 14 of November, CIBER-BBN  has celebrated its 11th Annual Conference in Hotel Santemar in Santander. In this conference there was a poster session with the participation of the following Units of NANBIOSIS. Special mention deserves Unit 1 with Neus Ferrer as Director and  Paolo Saccardo as Coordinator (in the picture):

Posters:

U1. Protein Production Platform (PPP):

Engineering protein complexes as nano- or micro-structured vehicles or drugs for human and veterinary medicine. Ugutz Unzueta, Naroa Serna, Laura Sánchez-García, José Vicente Carratalá, Olivia Cano-Garrido, Mercedes Márquez, Paolo Saccardo, Rosa Mendoza, Raquel Díaz, Héctor, López-Laguna, Julieta Sánchez, Anna Obando, Amanda Muñoz, Andrés Cisneros, Eric Voltà, Aida Carreño, José Luis Corchero, Neus Ferrer-Miralles, Esther Vázquez, Antonio Villaverde.

Units  U1. Protein Production Platform (PPP) and U18. Nanotoxicology Unit:

Intrinsic functional and architectonic heterogeneity of tumor-targeted protein nanoparticles. Mireia Pesarrodona, Eva Crosa, Rafael Cubarsi, Alejandro Sanchez-Chardi, Paolo Saccardo, Ugutz Unzueta, Fabian Rueda, Laura Sanchez-Garcia, Naroa Serna, Ramón Mangues, Neus Ferrer Miralles, Esther Vázquez, Antonio Villaverde.

Units U3. Synthesis of Peptides Unit, U6. Biomaterial Processing and Nanostructuring Unit, and U20. In Vivo Experimental Platform:

Synthesis of different length monodisperse COL-PEG-PEPTIDE to increase biodisponibility of multifunctional nanovesicles for Fabry’s desease. Edgar Cristóbal-Lecina; Daniel Pulido; Solène Passemard; Elizabet González-Mira; Jaume Veciana; Nora Ventosa; Simó Schwartz; Ibane Abasolo; Fernando Albericio and Miriam Royo.

Units U13. Tissue & Scaffold Characterization Unit and U17. Confocal Microscopy Service::

Preclinical behavior of medium-chain cyanoacrylate glue with two different surgical application forms for mesh fixation in abdominal wall repair. Gemma Pascual, Bárbara Pérez-Köhler, Marta Rodríguez, Claudia Mesa-Ciller, Ángel Ortillés, Estefanía Peña, Begoña Calvo, Juan M. Bellón.

Units U27. High Performance Computing and U8. Micro – Nano Technology Unit:

Inspiration and Expiration Dynamics in Acute Emotional Stress Assessment. Javier Milagro, Eduardo Gil, Jorge M. Garzón-Rey, Jordi Aguiló, Raquel Bailón.

U5. Rapid Prototyping Unit:

Poly-DL-lactic acid films functionalized with collagen IV as carrier substrata for corneal epithelial stem cells. Ana de la Mata, Miguel Ángel Mateos-Timoneda, Teresa Nieto-Miguel, Sara Galindo, Marina López-Paniagua, Xavier Puñet, Elisabeth Engel, Margarita Calonge.

U6. Biomaterial Processing and Nanostructuring Unit:

Strategy for engineering myoglobin nano-traps for biomedical sensing technology. E. Laukhina, O. V. Sinitsyna, N. K. Davydova, V. N. Sergeev, A. Gomez, I. Ratera, C. Blázquez Bondia, J. Paradowska, X. Rodriguez, J. Guasch, Jaume Veciana.

Structure and nanomechanics of quatsome membranes. B. Gumí-Audenis, L. PasquinaLemonche, J.A. Durán, N. Grimaldi, F. Sanz, J. Veciana, I. Ratera, N. Ventosa and M.I. Giannotti

U7. Nanotechnology Unit:

Bioreceptors nanostructuration study for early detection of Alzheimer. José Marrugo, Dr. Samuel Dulay, Dr. Mònica Mir, Prof. Josep Samitier.

RGD dendrimer-based nanopatterns promote chondrogenesis and intercellular communication for cartilage regeneration. Ignasi Casanellas, Anna Lagunas, Iro Tsintzou, Yolanda Vida, Daniel Collado, Ezequiel Pérez-Inestrosa, Cristina Rodríguez, Joana Magalhães, José A. Andrades, José Becerra, Josep Samitier.

Long-range electron transfer between redox partner proteins. Anna Lagunas, Alejandra GuerraCastellano, Alba Nin-Hill, Irene Díaz-Moreno, Miguel A. De la Rosa, Josep Samitier, Carme Rovira, Pau Gorostiza.

U8. Micro – Nano Technology Unit:

Miniaturized multi-sensing platform for pH and Dissolved Oxygen monitoring in Organ-On-aChip systems. M. Zea, A. Moya, I. Gimenez, R. Villa, G. Gabriel.

Electrochemical characterization of SWCNTs based microelectrodes fabricated by inkjet printing. M. Mass, A. Moya, G. Longinotti, M. Zea, M. Muñoz, E. Ramon, L. Fraigi, R. Villa, G. Ybarra, G. Gabriel.

U9. Synthesis of Nanoparticles Unit:

In vivo imaging and local persistance of polymeric micro- and nanomaterials labelled with the near infrared dye IR820. Isabel Ortiz de Solórzano, Gracia Mendoza, Inmaculada Pintre, Sara García-Salinas, Víctor Sebastián, Vanesa Andreu, Marina Gimeno, Manuel Arruebo.

U10. Drug Formulation:

Cationic nioplexes-in-polysaccharide-based hydrogels as versatile biodegradable hybrid materials to deliver nucleic acids. Santiago Grijalvo, Adele Alagia, Gustavo Puras, Jon Zárate, Judith Mayr, José Luis Pedraz, Ramon Eritja

U12. Nanostructured liquid characterization unit:

Perfluorocarbon-loaded Nanocapsules from Nano-emulsion Templates as Microbubble Precursors for Biomedical Applications. G. Calderó, A. González, M. Monge, C. Rodríguez-Abreu, M.J.García-Celma, C. Solans.

Biodistribution study of polymeric drug-loaded nanoparticles in murine model. Marta Monge, Aurora Dols, Stephane Fourcade, Aurora Pujol, Carlos Rodríguez-Abreu, Conxita Solans.

U16. Surface Characterization and Calorimetry Unit:

Behavior and a comparative study between tantalum and titanium alloy implant surfaces against bacterial adhesion. M.A. Pacha-Olivenza, M.L. González-Martín.

Bacterial adhesion on calcium ion-modified titanium implant surfaces. M.A. Pacha Olivenza, R. Tejero, M. Delgado-Rastrollo, M.L. González-Martín.

Bioactive coatings to promote tissue regeneration and ingrowth into 3D custom-made porous titanium endoimplants (COATREG-3D). Santos-Ruiz L; Granados JF; Ruiz F; Yáñez JI; González A; Cabeza N; Vida Y; Pérez-Inestrosa E; Izquierdo-Barba I; Vallet-Regí M; Rubio J; Orgaz F; Rubio N; González ML; Peris JL; Monopoli D; Becerra J.

U17. Confocal Microscopy Service:

Subcutaneous implantation of a biodegradable apatite/agarose scaffold: biocompatibility and osteogenesis characterization in a rat model. Natalio García-Honduvilla, Gemma Pascual, Miguel A. Ortega, Alejandro Coca, Cynthia Trejo, Jesús Román, Juan Peña, María V. Cabañas, Julia Buján, and María Vallet-Regí.

U25. NMR: Biomedical Applications I:

Dual T1/T2 NCP-based novel contrast agents for brain tumor MRI: a preclinical study. Suarez, S; Arias-Ramos, N; Candiota, AP; Lorenzo, J; Ruiz-Molina, D; Arús, C; Novio, F.

Metronomic treatment in immunocompetent preclinical GL261 glioblastoma: effects of cyclophosphamide and temozolomide. Ferrer-Font, L; Arias-Ramos, N; Lope-Piedrafita, S; Julià- Sapé, M; Pumarola, M; Arús, C; Candiota, AP.

U26. NMR: Biomedical Applications II:

Gated nanodevices for innovative medical therapies. Maria Alfonso, Irene Galiana, Beatriz Lozano, Borja Diaz de Greñu, Cristina de la Torre, Andrea Bernardos, Sameh El Sayed, Daniel MuñozEspin, Miguel Rovira, José Ramón Murguía, Manuel Serrano, Ramón Martínez-Máñez.

NANOPROBE: Gated sensing materials and devices for the detection of infectious diseases and urological cancer. Ángela Ribes, Luís Pla, Sara Santiago-Felipe, Alba Loras-Monfort, M.Carmen Martínez-Bisbal, Elena Aznar, Guillermo Quintás-Soriano, José Luis Ruiz-Cerdá, María Angeles.

 

 

 

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XI International Workshop on Sensors and Molecular Recognition: System for detection of senescent cells in vivo

The XI International Workshop on Sensors and Molecular Recognition will take place on 6 and 7 July 2017 at the Polytechnic University of Valencia. Scientists of the unit 26 of NANBIOSIS will present the results of the research carried on together with researchers from the Universitat Politècnica de València, CNIO, CIBER-BBN and the University of Cambridge: an innovative system that allows the detection of senescent cells in vivo and without damaging the tissue.

The main objective of cellular senescence is to prevent the proliferation of damaged cells and, at the same time, to trigger tissue repair. However, when the damage persists, or during aging, the tissue repair process is inefficient and the senescent cells tend to accumulate. This accumulation of senescent cells in the tissues affects the tissue functions and accelerates the aging.

“Elimination of senescent cells has been shown to improve a variety of diseases associated with aging, reverses degenerative processes and extends longevity. Therefore, the strategies to detect and eliminate senescent cells have gained great interest in recent years”, explains Manuel Serrano, principal investigator of the CNIO Tumor Suppression Group.

“Chemically speaking, the sensor is composed of a fluorophore bound to a galactose. Senescent cells have the differential property of breaking galactose bonds very efficiently. When the sensor is internalized in a senescent cell this link is broken and this results in a great increase in the fluorescence of the sensor, which is the signal that we detect excited with a laser. However, when the sensor is internalized in a normal (non-senescent) cell, no signal is observed, ” says Ramón Martínez-Máñez, Scientific Director of Unit 26 of NANBIOSIS, CIBER-BBN and IDM-UPV Institute.

The sensor has properties that make it possible to be excited by absorbing two photons, which causes that the energy of the laser used to visualize the tissues is much smaller than the conventional sensors. In addition, two-photon techniques decrease tissue damage and have greater penetrability.

“The sensor was injected intravenously into animals that had been treated with chemotherapy (which produced cellular damage and senescence), with a very selective signal being observed in regions that responded to chemotherapy (and therefore had many senescent cells) . The animals not treated with chemotherapy did not show any signs”, said Beatriz Lozano, researcher at the Interuniversity Institute for Research on Molecular Recognition and Technological Development (IDM) at the Universitat Politècnica de València

The probe, that  has been characterized in unit 26 of NANBIOSIS is potentially applicable to other models of senescence. Different research groups have already begun to test the probe with its biological models.

 

Article of reference:

Beatriz Lozano-Torres, Irene Galiana, Miguel Rovira, Eva Garrido, Selim Chaib Andrea Bernardos, Daniel Muñoz-Espín, Manuel Serrano, Ramón Martínez-Máñez and Félix Sancenón. An OFF–ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo J. Am. Chem. Soc. DOI: 10.1021/jacs.7b04985

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Nanoparticles capable of bi-directional communication

The results of a research co-led by Ramón Martínez-Máñez, Scientific Director of Unit 26 of NANBIOSIS and CIBER-BBN have been published by Nature Communication last May 30th, showing how to prepare a nanoparticles chemical communication system using lactose. So far, his group had managed to establish communication between three nanoparticles but only in a unidirectional way. “We have managed to get a nanoparticle to send a chemical messenger to the second nanoparticle, who understands the message and sends another chemical messenger back to the first one. Upon receiving it, it performs an action, in this case releasing a dye,” explains Martínez-Máñezto Efefuturo.

This is a very important “basic” research due to the lack of proven results at the nanoscopic level. This research is the first step in getting the nanoparticles to work in a collaborative and coordinated way, anticipating a future of intelligent nanoparticle networks and advanced controlled release systems, drugs or complex systems based on nanotechnology.

The characterization of the system has been done with NMR unit of NANBIOSIS

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Electronic tongues for detecting prostate and bladder cancer

Researchers of the Applied Molecular Chemistry Group, coordinator of Unit 26 of NANBIOSIS, have participated in the development of a new low-cost system for non-invasive diagnosis of prostate and bladder cancer. It consists of a device of electronic tongues ​​based on metal electrodes, which makes it possible to quickly and easily detect this pathology from a urine sample.

The researchers evaluated the efficacy of this system from the analysis of urine samples from patients before and after surgery. In addition, samples were collected from patients with benign prostatic hyperplasia, integrated into the non-cancer group for the study of prostate cancer. This system was able to distinguish non-cancerous urine samples from the affected ones with a sensitivity of 91% and a specificity of 73%.

The specificity and sensitivity obtained by the electronic tongues in urine is higher compared to the prostate-specific PSA-blood test, which is the most commonly used procedure for the detection of prostate cancer. “The results obtained confirm the suitability of this technology of electronic tongues ​​for the identification of patients affected by this pathology. This technology has great potential for its application in clinical practice, both for the diagnosis and for monitoring the evolution of patients after therapy”, said Ramón Martínez Máñez, Scientific Director of NANBIOSIS.

The measurement of electronic tongues on the urine is done by putting the sensor, in this case composed of a set of noble and semi-precious metals, with the urine sample of the patient. It is connected to a potentiostat that applies different potentials to the electrodes and, in turn, collects the resulting currents to be analysed in a computer equipped with a computer program for multivariate analysis.

“The tongue is” trained” in a first phase with a set of patient samples and controls to generate a model that discriminates between both types of samples. That model, once validated, could be used to predict new urine samples and to be able to determine whether or not these new patients have the disease with a certain margin of sensitivity and specificity”, explains Ramón Martínez Máñez.

Potential metabolites recognized by the electronic tongue were studies by NMR using the NANBIOSIS-ICTS

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New sensor for quick and easy detection of ecstasy

Spanish, Danes and Austrian researchers, including the group led by Ramón Martínez Mánez, coordinator of Unit 26 of NANBIOSIS, have developed a new sensor to detect ecstasy in a simple, reliable, fast and selective way.

Until now, this drug is detected by techniques such as capillary electrophoresis, spectroscopy or chromatography that require the use of expensive technical equipment and qualified personnel. The new method offers the advantages of easy use, low cost and high reliability according to Beatriz Lozano, a researcher at the Interuniversity Institute for Research on Molecular Recognition and Technological Development at the Universitat Politècnica de València and the group led by Ramón Martínez Mañez.

The sensor consists of a hybrid material including an inorganic carrier charged with a dye -fluorescein- and functionalized with an organic molecule derived from naphthol.

The dye is retained when the bluebox-molecule widely used in chemistry- is coordinated to the naphthol forming a molecular gate and in the presence of ecstasy in the analyzed sample, a variation of the fluorescence occurs, whose intensity varies as a function of the concentration of Drug detected.

“The affinity of BlueBox for different neurotransmitters such as dopamine had already been described, and because of the structural similarity between that neurotransmitter and ecstasy, we thought it would be a good idea to try to open the “molecular gate” in the presence of the drug and release the dye that gives the fluorogenic response”, explains Beatriz Lozano.

The characterization of the products by NMR was carried out by using facilities from NANBIOSIS-ICTS (unit 26)

Researchers are currently working on a new sensor for the detection of other synthetic drugs.

Reference Article:

Beatriz Lozano-Torres, Lluís Pascual, Andrea Bernardos, María D. Marcos, Jan O. Jeppesen, Yolanda Salinas, Ramón Martínez-Máñez and Felix Sancenón. Pseudorotaxane capped mesoporous silica nanoparticles for 3,4-methylenedioxymethamphetamine (MDMA) in water detection. Chem. Commun., 2017, 53, 3559. DOI: 10.1039 / C7CC00186J

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Laboratory-scale prototype for the treatment of cutaneous tumours

Ramon Martínez Máñez, Scientific Director of Unit 26 of NANBIOSIS, has participated in the development, on a laboratory scale, of a low cost device for treatments based on the application of optical hyperthermia by laser.

Among the applications of this technique are the therapies against cutaneous cancer and its objective is to get the death of the tumour cells by overheating. Overheating is achieved by the irradiation of synthesized metal nanoparticles.

The prototype developed consists of an infrared laser with a power of up to 500mW able to provide a power density of up to 4W / cm2, a sensor that allows recording the temperature in real time during the irradiation and a power regulator of the laser, among other components.

The work shows the keys for the development of low cost equipment for the technique of optical hyperthermia. The main novelty with respect to other commercial equipments is that it integrates all the necessary elements for the experimental phase, controlling as many variables as possible.

Currently, there are different laser applicators, used in dermatology and surgery. At certain powers and wavelengths, the energy of the laser is transformed into heat and produces ablation (burn), with the adjacent inflammation that this causes. The prototype is intended to introduce to sick cells nanohaters that, when stimulated by the laser, raise their temperature to 42-48 °, producing hypoxia.

This equipment is already being used successfully in cell cultures in vitro and is also working on therapies in which hyperthermia is combined with controlled release of drugs. Once the technique was developed, it could easily be transferred to a hospital environment by implementing small changes.

Article of reference:

Roberto Montes-Robles, Andy Hernandez, Javier Ibáñez, Rafael Masot-Peris, Cristina de la Torre, Ramón Martínez-Máñez, Eduardo García-Breijo, Rubén Fraile. Design of a low-cost equipment for optical hyperthermia. Sensors and Actuators A: Physical.

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New implants for the treatment of infections and bone tumours

Researchers of Unit 26 of NANBIOSIS participate in the design and evaluation of the cellular level of a new hybrid material for the creation of intelligent-scaffolds implants that open the door to a more efficient treatment of infections and bone tumors.

The main novelty is the incorporation of a mesoporous matrix of silicon, calcium and phosphorus oxides, with molecular gates that would allow the controlled release of substances stored inside the pores, such as antitumor drugs or antibiotics. A molecular gate is a mechanism whose opening depends on certain external stimuli, in this particular case the drugs would only be released in the presence of enzymes associated with a tumor or a infection in the bones.Nowadays there are scaffolds that allow the slow release of a drug, but this is produced automatically and non-specific.

Molecular gates have been characterized by the use of the Nuclear Magnetic Resonance Unit 26 of NANBIOSIS

 

Article of reference:

Lorena Polo, Natividad Gómez-Cerezo, Elena Aznar, José-Luis Vivancos, Félix Sancenón. Daniel Arcos, MaríaVallet-Regí, Ramón Martínez-Máñez. Molecular gates in mesoporous bioactive glasses for the treatment of bone tumors and infection. Acta Biomaterialia. http://dx.doi.org/10.1016/j.actbio.2016.12.025.

 

U 26 NANBIOSIS - New implants for the treatment of infections and bone tumours
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Ramón Martínez, Scientific Director of Unit 26 of NANBIOSIS Co-leads the development of nanodevices to detect the presence of cocaine

Researchers of the Universitat Politècnica de València (UPV), the Universitat Rovira i Virgili (URV) and CIBER of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) have developed new nanodevices to detect the presence of cocaine in saliva or in any another aqueous solution. Part of the characterization of the probe was carried out using NMR facilities from NANBIOSIS. The paper is published in the journal Scientific Reports, of the group Nature.

The development consists of a molecular gate designed with nanoporous alumina, an aluminum oxide prepared by electrochemical means with pores of micrometric diameter. “A molecular gate -explains Ramón Martínez-Máñez, scientific director of Unit 26 of NANBIOSIS- is a mechanism in which its state (open or closed) can be controlled at will by external stimuli, in this case the presence of cocaine. The main novelty with respect to other detection systems is the use of porous alumina plates that are easy to use and reusable for other sensors”.

This new intelligent nanodispositive lays the groundwork for the use of porous alumina in the development of molecular gates for the detection of chemical and biological compounds with applications in fields such as health and the environment.

Article of reference:
Nanbiosis U26 - ribes y martinez-manez development of nanodevices to detect the presence of cocaine
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4 Units of NANBIOSIS hosted in three Universities among the 200 best in the world in science.

The University of Zaragoza is one of the 200 best universities in the world in science research, according to the latest Shanghai ranking of 2016 in a specific analysis by macro areas.

In order to elaborate this ranking by macroareas indicators such as the number of students and professors who have obtained the Nobel prize or Fields medal, the number of researchers highly cited in the scientific literature, the number of scientific articles indexed in the Science Citation Index and the Percentage of articles published in the most cited 20% scientific journals are used.

Only six Spanish universities are among the 200 best in the world in the field of science, such as the Autonomous University of Madrid, University of Barcelona, University of Santiago de Compostela, Polytechnic University of Valencia, University of Valencia and Zaragoza.

The list of the world’s top 200 universities in science is headed by American universities in Berkeley (California), Stanford, Princeton, Harvard, the Massachusetts Institute of Technology and California, which rank the top six. In the seventh one appears the first European university, Cambridge, followed by the one of Tokyo, Technological Institute of Switzerland and the University of Los Angeles.

NANBIOSIS contributes to this success through the elite researchers that coordinate three of its Units located in the University of Zaragoza: Unit 9 that offers services for sciences research as Synthesis of Nanoparticles biomedicine, micronanotechnology or microfluidics biomedicine, Unit 13 with services as tissue and biomaterial characterization or mechanical tests of tissues and Unit 27 which offers services for sciences research on biomedical computer simulation, modelling of biomedical systems or large simulation multicore and multiserver, among other aspects.

In addition, the Polytechnic University of Valencia and the University of Valencia provides the scientific management and part of the equipment to Unit 26 of NANBIOSIS that offers services for research as molecular imaging metabolomics by NMR or metabolic profiling of biofluids by NMR

Three Universities among the 200 best in the world in science, hosting 4 Units of NANBIOSIS.
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A new system to detect Cocaine at very low concentrations

Ramon Martínez Máñez, Scientific Director of the Unit 26 of NANBIOSIS, together with researchers from his group and the group led by Professor Liz Marzal, both CIBER-BBN groups, have developed a new method to detect cocaine and Mycoplasma in very low concentrations.

The method involves the combined use of mesoporous silica nanoparticles, molecular equipped with doors, and spectroscopy SERS (Surface-Enhanced Raman Scattering) a signal amplification system that uses gold nanoparticles to detect very low concentrations of the substances tested. According to the tests that have been developed in the laboratory, in the case of cocaine it has allowed them to nanomolar levels of detection, and the Mycoplasma genomic DNA 30 copies/uL.

The detection system is based on the release of a dye easily identifiable spectroscopy SERS from inside silica nanoparticles, only when the species to be detected is present. “The pores of the nanoparticles are unblocked in the presence of cocaine and Mycoplasma or a dye that interacts with gold nanotriángulos is released, and is this interaction which is detected by SERS spectroscopy. The concentration of the substance to be detected is proportional to the detected signal”, says Ramon Martinez Máñez, Scientific Director of NANBIOSIS.

Article of reference:

Surface Enhanced Raman Scattering and Materials for Sensing Applications Gated: The Ultrasensitive Detection of Mycoplasma and Cocaine. Oroval, M., Coronado-Püchau, M. Langer, J. Sanz-Ortiz, MN Ribes, A. Aznar, E. Coll, C. Marcos, M.D. Sancenón, F. Liz-Marzan, L. M., Martinez-Máñez, R. Chemistry-A European Journal. DOI: 10.1002 / chem.201602457

A new system to detect Cocaine at very low concentrations
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