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Equipments

Equipments

U29-E03. Speed-Vac evaporators

Speed-Vac evaporators for removal of water in oligonucleotide samples

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U29-E04. UV spectrophotometer equipped with a Peltier temperature gradient.

UV-Visible spectrophotometer equipped with a Peltier temperature gradient to run thermal denaturation assays followed by UV absorption.

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U29-E05. Spectrofluorimeter equipped with a Peltier temperature gradient.

Spectrofluorimeter equipped with a Peltier temperature gradient to run thermal denaturation assays and characterization of DNA-binding drugs followed by fluorescence.

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U28-E19. Beckman Coulter MoFlo cell sorter

Specifications:
Validated for 70,000 sorts per second with > 99% purity at all data rates
Proven 4-Way Sort for multiple population sorting
Independent control of each stream via touch screen control panel
High power solid state 488nm and 638nm lasers

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U28-E18. TRIMODAL NUCLEAR IMAGING EQUIPMENT MICROTOMOGRAPHY SYSTEM WITH FUNCTIONAL IMAGING TECHNOLOGY BY POSITRON EMISSION TOMOGRAPHY (PET) AND BY SINGLE PHOTON EMISSION TOMOGRAPHY (SPECT)”)

Specifications:

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U28-E17. Perkin Elmer Operetta HCS confocal

U28-E17. Perkin Elmer Operetta HCS confocal

Specifications:
Spinning disc confocal technology
10x, 20x and 40x long working distance objectives
20x high NA objectives
Temperature control and CO2 for cultured cells
à 400W Xenon based illumination
Fully automated image acquisition from 24,96 and 384 well plates
Harmony: integrated acquisition and analysis software
Phenologic – machine learning-based phenotype recognition software
Columbus Image Data Storage and Analysis System

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U28-E16. Nikon Eclipse Ti basic Fluorescence Microscope

U28-E16. Nikon Eclipse Ti basic Fluorescence Microscope

Specifications:
Inverted Nikon Ti Eclipse fully motorised microscope
Hamamatsu Orca R2 monochrome 1.3 MP CCD camera
Okolab H101 stage-top incubator with temperature, humidity and CO2 control
Objective lenses:

  • Plan Apo Oil immersion: 60x (1.4 NA)
  • Super Plan Fluor 40x (0.6 NA) Extra Long working distance (2.8-3.6mm)
  • Plan Apo 10x (0.45 NA)

Nikon Intensilight 130w Mercury Lamp
Filter sets for DAPI, GFP, TRITC
Nikon PFS (Perfect Focus System) for improved stability

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U28-E15. Nikon Eclipse Ti TIRF Fluorescence Microscope + N-Storm

U28-E15. Nikon Eclipse Ti TIRF Fluorescence Microscope + N-Storm

Specifications:
Inverted Nikon Ti Eclipse fully motorised microscope
100x Plan Apo Oil immersion Objective 1.49 NA
TIRF optical system (used to improve contrast with N-STORM samples)
Motorised TIRF mirror
Multipass TIRF dichroic filter (allows high-speed multicolour TIRF)
Andor iXon3 897 EM-CCD camera
Microscope enclosure with temperature, humidity and CO2 control
High precision motorized stage.
Nikon PFS (Perfect Focus System) for improved stability
Offline workstation with additional processing module
Objective lenses:

  • Plan Apo Oil immersion: 100x (1.49 NA) TIRF
  • Plan Fluor 40x (0.75 NA)
  • Plan Apo 10x (0.45 NA).
  • Removal cylindrical lens for 3D super-resolution

4 Lasers in the visible range:

  • Violet 405nm
  • Argon-Ion 457nm, 477nm, 488nm, 514nm
  • Yellow Diode 561nm
  • Red 647nm
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U28-E14. Leica SP5 HyD MP Multiphoton Confocal Microscope

U28-E14. Leica SP5 HyD MP Multiphoton Confocal Microscope

Specifications:
Inverted Leica DMI 6000 fully motorised microscope
Confocal detectors: 2 x HyD, 3 x PMT + 1 transmitted light detector
Tandem Scanner – HQ conventional scanner and high-speed Resonant scanner (28 frames/sec @ 512 x 512)
AOBS (Acousto-Optical beam splitter) – brighter and more flexible than con-ventional beam splitters
Optical zoom up to 64x (maximum resolution is dependent on the objective used)
Choice of objectives:

  • Plan Apo Oil immersion: 100x (1.4 NA), 63x (1.4 NA Blue), 40x (1.3 NA)
  • Plan Apo 20x (0.7 NA) multi-immersion (water, glycerol, oil) 0.26mm WD
  • Plan Apo 10x (0.4 NA) dry
  • Plan Apo 25x (0.95 NA) water immersion, IR corrected for multiphoton, 2.2mm WD

9 Laser lines in the visible range:

  • Violet 405nm (50mW), Blue Argon 458nm, 476nm, 488nm, 496nm, 514nm (65mW) Green DPSS 561nm (20mW), Orange HeNe 594nm (2mW), Red HeNe 633nm.

High Performance Mai-Tai titanium-sapphire IR femtosecond pulse laser for multi-photon imaging, tunable from 690-1040nm (maximum power 800nm∼ 2.9W)
Microscope enclosure with temperature, humidity and CO2 control
High precision Super Z motorized stage with multi-point and multi-tile soft-ware control. 3nm resolution galvometric z-axis control

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U28-E13. Leica SP5 HyD Confocal Microscope

Specifications:
Inverted Leica DMI 6000 fully motorised microscope
Confocal detectors: 2 x HyD, 3 x PMT + 1 transmitted light detector
Tandem Scanner – HQ conventional scanner and high-speed Resonant scanner (28 frames/sec @ 512 x 512)
AOBS (Acousto-Optical beam splitter) – brighter and more flexible than con-ventional beam splitters
Optical zoom up to 64x (maximum resolution is dependent on the objective used)
Choice of objectives:

  • Plan Apo Oil immersion: 100x (1.4 NA), 63x (1.4 NA Blue), 40x (1.3 NA)
  • Plan Apo 20x (0.7 NA) multi-immersion (water, glycerol, oil) 0.26mm WD
  • Plan Apo 10x (0.4 NA) dry

9 Laser lines in the visible range:

  • Violet 405nm (50mW), Blue Argon 458nm, 476nm, 488nm, 496nm, 514nm (65mW), Green DPSS 561nm (20mW), Orange HeNe 594nm (2mW), Red HeNe 633nm

Microscope enclosure with temperature, humidity and CO2 control
High precision Super Z motorized stage with multi-point and multi-tile soft-ware control. 3nm resolution galvometric z-axis control

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