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U3-S01. Synthesis of peptides and characterisation (On-site&Remote) OUTSTANDING

Synthesis of peptides and characterisation (On-site&Remote) OUTSTANDING

Peptide production on various scales from mg (scale: 10 mg, 50 mg and 100 mg) and grams.
Synthesis of peptides containing phosphorylated amino acids, acylated or methylated side chains (lysine) or non-natural amino acids.
Peptides can be delivered as synthesis crudes (without purification) and purified (≥95% by HPLC).
Peptides are characterized by HPLC and HPLC-MS

Customer benefits

  • Extensive experience on peptide synthesis.
  • Experience in difficult sequences.
  • Control quality at different synthesis points.
  • Capability to design diverse synthesis strategies and purification
  • Development of peptide synthesis methodology.

Target customer

  • Research groups (drug delivery, molecular biology, pharmacology, nanotechnology, biotechnology)
  • Companies (biotech and pharma companies).

References

  • Pharmacological activation of insulin-degrading enzyme improves insulin secretion and glucose tolerance in diet-induced obese mice. Sanz-Gonzalez, Alba; Cozar-Castellano, Irene; Broca, Christophe ; Sabatier, Julia; Acosta, Gerardo A. ; Royo, Miriam ; Hernando-Munoz, Carla; Torroba, Tomas ; Perdomo, German ; Merino, Beatriz. Diabetes, Obesity and Metabolism (2023), 25, 3268-3278.
  • Amide Formation: Choosing the Safer Carbodiimide in Combination with OxymaPure to Avoid HCN Release. Manne, Srinivasa Rao; Luna, Omar; Acosta, Gerardo A.; Royo, Miriam ; El-Faham, Ayman ; Orosz, Gyorgy; de la Torre, Beatriz G. ; Albericio, Fernando. Organic Letters (2021), 23, 6900-6904.
  • Carbosilane Dendron-Peptide Nanoconjugates as Antimicrobial Agents
  • By: Fernandez, Jael; Acosta, Gerardo; Pulido, Daniel; Maly, Marek; Copa-Patino, Jose L.; Soliveri, Juan; Royo, Miriam; Gomez, Rafael; Albericio, Fernando; Ortega, Paula; de la Mata, F. Javier. Molecular Pharmaceutics (2019), 16, 2661-2674.
  • Optimized Stepwise Synthesis of the API Liraglutide Using BAL Resin and Pseudoprolines. Carbajo, Daniel; El-Faham, Ayman; Royo, Miriam; Albericio, Fernando. ACS Omega (2019), 4, 8674-8680
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U6-E10. Nanosight NS-300 for Nanoparticle Tracking Analysis by fluorescence mode

Nanosight NS300; Malvern Instruments

Descripción: Automated analysis of the size distribution and concentration

The Malvern NanoSight NS300 uses the technology of Nanoparticle Tracking Analysis (NTA). This unique technology utilizes the properties of both light scattering and Brownian motion in order to obtain the size distribution and concentration measurement of particles in liquid suspension. A laser beam is passed through the sample chamber, and the particles in suspension in the path of this beam scatter light in such a manner that they can easily be visualized via 20x magnification microscope onto which is mounted a camera. The camera operates at 30 frames per second (fps), capturing a video file of the particles moving under Brownian motion. The software tracks many particles individually and using the Stokes-Einstein equation calculates their hydrodynamic diameters.

More info:  http://www.malvern.com/en/products/product-range/nanosight-range/nanosight-ns300/default.aspx

Technical Specifications:

Wavelength: 405nm (violet), 488nm (blue), 532nm (green) or 642nm (red).

Temperature control range: 5°C below ambient to 55°C.

Stage: Fixed stage.

Focus: Computer controlled motorized focus.

Camera:

Fluorescence: Motorized 6 place filter wheel with choice of filters.

Particle size: 10nm to 2000nm.

Concentration range: 106to 109 particles per mL.

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U6-S14. Quantitative studies of biomolecular interactions by calorimetric measurements (On-site) OUTSTANDING

Quantitative studies of biomolecular interactions by calorimetric measurements

Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event (protein-small molecule, protein-protein, target-drug, enzyme-inhibitor, antibody-antigen, protein-DNA, protein-lipid, small molecule-small molecule). Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment. Because ITC goes beyond binding affinities and can elucidate the mechanism of the molecular interaction, it has become the method of choice for characterizing biomolecular interactions.
The equipment used for this purpose is VP-ITC (GE HealthCare-Microcal).

Customer benefits

Applications range goes from drug design to fundamental research, such as understanding and regulating signal transduction pathways. These systems provide direct marker-free and in-solution measurement of binding affinity and thermodynamic parameters in a single experiment. They have high sensitivity, low sample consumption and automation options to minimise handling time.

Target customer

  • Biochemical and Pharmaceutical companies.
  • Biology and biochemistry research groups.

Additional information

M.Köber, et al., Journal of Colloid and Interface Science 631 (2023) 202–211. DOI: 10.1016/j.jcis.2022.10.104

Compra al mejor precio MALVERN MicroCal VP-ITC | Bimedis

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