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U14-S02. Flow Cytometry

Flow Cytometry

The Flow Cytometry service occupies a leading position in biomedical and pharmaceutical research, as well as in cellular and molecular biology. Utilizing cutting-edge instrumentation and specialized expertise, this service offers a diverse array of analytical techniques crucial for elucidating the nuanced intricacies of cellular behavior. Ranging from precise cellular phenotyping to dynamic proliferation assays, and encompassing assessments of viability alongside in-depth investigations into apoptosis, our service provides a comprehensive toolkit for the thorough interrogation of cellular characteristics and responses.

Customer benefits

U14 Cell Therapy Unit offers researchers precision, efficiency, and expertise, streamlining experiment. With stringent quality control, ISO 9001-2015 and GLP compliant, tailored solutions, and access to advanced equipment, researchers can focus on innovation and core objectives, driving scientific progress forward.

Target customer

This service is essential for organizations involved in Research and Development, including pharmaceutical companies, academic institutions for biomedical research, biotechnology firms for bioprocessing, and regulatory agencies for in vitro studies.
Through the precise analysis enabled by flow cytometry, researchers gain invaluable insights into cellular behavior, facilitating the advancement of preclinical trials and therapeutic development.

Additional information

Selected references:

  • de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther.  2023.  Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551.  https://doi.org/10.1186/s10020-023-00646-1
  • Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM.  2022.  Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells   Animals.  MDPI.  12-20.  ISSN 2076-2615.  https://doi.org/10.3390/ani12202798
  • Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther.  2021.  A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
  • de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10).  2021.  IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.  MDPI.  22-22.  ISSN 1422-0067.  WOS (0) https://doi.org/10.3390/ijms222212177
  • López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269.  https://doi.org/10.1007/s12015-019-09926-y
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U14-S01. Cell cultures

Cell cultures

The cell culture service plays a pivotal role in the realm of biomedical and pharmaceutical research, as well as in the broader fields of cellular and molecular biology. It provides specialized support for the development, production, and characterization of cell therapies tailored for preclinical trials. With a focus on mesenchymal stem cells (MSCs), the service encompasses the entire spectrum of cell culture activities, including isolation, expansion, and thorough characterization of these crucial cellular components. Additionally, the service extends its offerings to include essential assays such as proliferation, viability, and cytotoxicity assessments, all of which are indispensable in the rigorous evaluation of potential therapies during preclinical trials.

Customer benefits

U14 Cell Therapy Unit offers researchers precision, efficiency, and expertise, streamlining experiments. With stringent quality control, ISO 9001-2015 and GLP compliant, tailored solutions, and access to advanced equipment, researchers can focus on innovation and core objectives, driving scientific progress forward.

Target customer

This service is essential for organizations involved in Research and Development, including pharmaceutical companies, academic institutions for biomedical research, biotechnology firms for bioprocessing, and regulatory agencies for in vitro studies. Cell Culture service empowers researchers and stakeholders with the tools and insights necessary to advance the frontiers of regenerative medicine and therapeutic innovation.

Additional information

Selected references:

  • de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther.  2023.  Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551.  https://doi.org/10.1186/s10020-023-00646-1
  • Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM.  2022.  Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells   Animals.  MDPI.  12-20.  ISSN 2076-2615.  https://doi.org/10.3390/ani12202798
  • Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther.  2021.  A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
  • de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10).  2021.  IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.  MDPI.  22-22.  ISSN 1422-0067.  WOS (0) https://doi.org/10.3390/ijms222212177
  • López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269.  https://doi.org/10.1007/s12015-019-09926-y
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U14-E01. Cell culture rooms

Cell Culture Room 1:
MSC Advantage Class II b Biological Safety Cabinet (Thermo)
CO2 incubator Heracell 150 (Thermo)
Nikon Eclipse TE2000 fluorescence inverted microscope (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)

Cell Culture Room 2:
2 MSC Advantage Class II b Biological Safety Cabinets (Thermo)
2 CO2 incubators Heracell 150 (Thermo)
Phases inverted microscope (NIKON)
2 Vacuum Pumps ME4 (Vacuubrand)
Cell Counter (Invitrogen)

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U14-E02. Molecular biology

MACSQuant 10 Analizer Flow Cytometer (Miltenyi Biotec)

Microplate reader Synergy MX (BIOTEK)

QuantStudio 3 Real-Time PCR System (AppliedBiosistem)

UV/Vis Spectrophotometer (IMPLEN)

Thermal Cycler PXE 0.2 (THERMO)

Fume Hood Nublar NBL-1600 (Formimetal)

Refrigerated centrifugeMultifuge 1 (Heraeus)

Refrigerated centrifuge for microtubes Heraeus Fresco 21 (Thermo)

Rotary shaker with incubation (HEIDOLPH)

Digital circulation thermostatic bath (GRANT)

Manual variable volume pipettes (Eppendorf, Kartell, Rainin)

Multichannel variable volume pipettes (Thermo)

Pipette controllers (Gosselin, Rainin, Lab box)

Liquid Nitrogen tank (Taylor Wharton)

Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80°C) (HERAEUS)

Freezers -20°C (FIOCHETTI, Indesic)

Refrigerators +2/+8ºC (Thermo, CLASS ELECTRONIC)

Thermoblock Accublock (Labnet)

Heating magnetic stirrer ARE (VELP Scientifica)

Analytical balance (Metria)

Precision analytical balance Pioneer (OHAUS)

pH-Meter Symphony (VWR)

Ultrasonic water bath (SELECTA)

Vortex 230 V, EU (VWR)

Microwave (LG)

Crushed ice machine (Schottman)

Autoclave Presoclave-II (P SELECTA)

Sterilization incubator (Modelo UT ) (HERAEUS)

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U13-E02. Machines for mechanical tests

Machines for mechanical tests:

• Instron MicroTester 5848 and 5548: electromechanical machines for displacement or load control tests:

•• load range from 1mN to 2kN at full scale.
•• load cells of:+/- 5, 50, 500 and 1000N.
•• synchronous data capture in all the channels up to
500 Hz.
• Biaxial Instron MicroTester 8874: axial-torsional, servohydraulic testing machine:
•• load capacity of 25 kN and 100 Nm.
•• fatigue tests at a low number of cycles.

›› Software for data control and analysis.
›› Accessories and tools especially designed by the group, which allow a wide flexibility in designing tests, allowing the verification and/or validation of theoretical models.
›› AVE video extensometer connected to the machines; resolution of 0.5 μm. To determine the axial and transverse deformation without contact (tension, dynamic properties and viscoelasticity) of the samples.

• E1000 Test Instrument: Dynamic systems to offer slow-speed static testing and high-frequency dynamic fatigue testing with hundreds of Hertz capability: Load range: 250 N to 2.0 KN.
• Nano BIONIX MTS: For nanomechanical characterization of biomaterials. Load range to 0.001N to 0.5N.

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U13-E01. MicroCT GE eXplore Locus SP (GE)

MicroCT GE eXplore Locus SP (GE): Computed X-ray Axial Tomograph for the non-destructive study of the microstructure and in vitro tomography: ›› Samples of up to 40 mm in diameter; resolution of 10 μm. ›› Different types of tissues, implants, scaffolds, etc. ›› Complete “section-by-section” imaging with a single rotation (cone beam volumetric technology); excellent image quality and “signal-to-noise” ratio. ›› “Bone Analysis Tool” software provided with: •• BMD (Bone Mineral Density): It calculates the density of minerals in the bone, the bone volume fraction and the mineral content in the bone. •• Anisotropy: It determines the degree of symmetry and the orientation of the trabecular structure. •• Cortical Analysis: It determines “section-by-section” the width, surface area and the BMD value of the cortical bone. •• Stereology: It provides the bone volume fraction, the ratio between the surface of the bone and its volume and the width of the trabecular plate. •• Direct Measures: It determines the local trabecular width of a bone and allows obtaining a visual representation of this local width.

Temporarily OUT OF ORDER

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U13-S01. MicroCT

MicroCT

X-Ray image for biomaterials, scaffolds and biological tissues.

Customer benefits

An axis inside is able to apply forces.
Non-destructive view of internal structure pre and post force.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

Additional information

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U13-S03. Fatigue tests Instron 8874

Fatigue tests Instron 8874

Fatigue tests allow us to know how is the behaviour when cyclic load are applied. It is used for life expectancy of biomaterials and biological tissues.

Customer benefits

High full-scale cell load.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

Additional information

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U13-S04. Dynamic mechanical analysis Instron 8874

Dynamic mechanical analysis Instron 8874

Dynamic tests allow us to know how is the behaviour along time. It is used for viscoelastic properties of biomaterials and biological tissues.

Customer benefits

High full-scale cell load.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

Additional information

D:\gestion\Laboratorio\espacios\fotos_plat\INSTRON 8874\P1000356.JPG

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U13-S05. Uniaxial test Instron MicroTester

Uniaxial test Instron MicroTester

Tensile, pure shear and planar tension are the tests inside the uniaxial group. Samples are stretched along an axis by measuring force and displacement. Cycling protocols can be integrated into the mechanical test. Mechanical and viscoelastic properties can be studied. Force is measured by means of load cells.

Customer benefits

  • Load cells are available with different full-scales depending on the material to test.
  • Tests can be controlled by means of displacement or force.
  • Specific protocols according customer requirements.
  • Tracking system can be used to measure real deformations.
  • Adaptable clamps according to sample geometry.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

References

  • Ríos-Ruiz, I., Martínez, M.Á. & Peña, E. Is location a significant parameter in the layer dependent dissection properties of the aorta?. Biomech Model Mechanobiol 21, 1887–1901 (2022). Doi: 10.1007/s10237-022-01627-9
  • Peña E, Alastrué V, Laborda A, Martínez MA, Doblaré M. A constitutive formulation of vascular tissue mechanics including viscoelasticity and softening behaviour. J Biomech. 2010 Mar 22;43(5):984-9. doi: 10.1016/j.jbiomech.2009.10.046. Epub 2009 Dec 2. PMID: 19959171.

Additional information

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