Posts by Nanbiosis

U28-E16. Nikon Eclipse Ti basic Fluorescence Microscope

Specifications:
Inverted Nikon Ti Eclipse fully motorised microscope
Hamamatsu Orca R2 monochrome 1.3 MP CCD camera
Okolab H101 stage-top incubator with temperature, humidity and CO2 control
Objective lenses:

• Plan Apo Oil immersion: 60x (1.4 NA)
• Super Plan Fluor 40x (0.6 NA) Extra Long working distance (2.8-3.6mm)
• Plan Apo 10x (0.45 NA)

Nikon Intensilight 130w Mercury Lamp
Filter sets for DAPI, GFP, TRITC
Nikon PFS (Perfect Focus System) for improved stability

U28-E15. Nikon Eclipse Ti TIRF Fluorescence Microscope + N-Storm

Specifications:
Inverted Nikon Ti Eclipse fully motorised microscope
100x Plan Apo Oil immersion Objective 1.49 NA
TIRF optical system (used to improve contrast with N-STORM samples)
Motorised TIRF mirror
Multipass TIRF dichroic filter (allows high-speed multicolour TIRF)
Andor iXon3 897 EM-CCD camera
Microscope enclosure with temperature, humidity and CO2 control
High precision motorized stage.
Nikon PFS (Perfect Focus System) for improved stability
Offline workstation with additional processing module
Objective lenses:

• Plan Apo Oil immersion: 100x (1.49 NA) TIRF
• Plan Fluor 40x (0.75 NA)
• Plan Apo 10x (0.45 NA).
• Removal cylindrical lens for 3D super-resolution

4 Lasers in the visible range:

• Violet 405nm
• Argon-Ion 457nm, 477nm, 488nm, 514nm
• Yellow Diode 561nm
• Red 647nm

U28-E14. Leica SP5 HyD MP Multiphoton Confocal Microscope

Specifications:
Inverted Leica DMI 6000 fully motorised microscope
Confocal detectors: 2 x HyD, 3 x PMT + 1 transmitted light detector
Tandem Scanner – HQ conventional scanner and high-speed Resonant scanner (28 frames/sec @ 512 x 512)
AOBS (Acousto-Optical beam splitter) – brighter and more flexible than con-ventional beam splitters
Optical zoom up to 64x (maximum resolution is dependent on the objective used)
Choice of objectives:

• Plan Apo Oil immersion: 100x (1.4 NA), 63x (1.4 NA Blue), 40x (1.3 NA)
• Plan Apo 20x (0.7 NA) multi-immersion (water, glycerol, oil) 0.26mm WD
• Plan Apo 10x (0.4 NA) dry
• Plan Apo 25x (0.95 NA) water immersion, IR corrected for multiphoton, 2.2mm WD

9 Laser lines in the visible range:

• Violet 405nm (50mW), Blue Argon 458nm, 476nm, 488nm, 496nm, 514nm (65mW) Green DPSS 561nm (20mW), Orange HeNe 594nm (2mW), Red HeNe 633nm.

High Performance Mai-Tai titanium-sapphire IR femtosecond pulse laser for multi-photon imaging, tunable from 690-1040nm (maximum power 800nm∼ 2.9W)
Microscope enclosure with temperature, humidity and CO2 control
High precision Super Z motorized stage with multi-point and multi-tile soft-ware control. 3nm resolution galvometric z-axis control

U28-E13. Leica SP5 HyD Confocal Microscope

Specifications:
Inverted Leica DMI 6000 fully motorised microscope
Confocal detectors: 2 x HyD, 3 x PMT + 1 transmitted light detector
Tandem Scanner – HQ conventional scanner and high-speed Resonant scanner (28 frames/sec @ 512 x 512)
AOBS (Acousto-Optical beam splitter) – brighter and more flexible than con-ventional beam splitters
Optical zoom up to 64x (maximum resolution is dependent on the objective used)
Choice of objectives:

• Plan Apo Oil immersion: 100x (1.4 NA), 63x (1.4 NA Blue), 40x (1.3 NA)
• Plan Apo 20x (0.7 NA) multi-immersion (water, glycerol, oil) 0.26mm WD
• Plan Apo 10x (0.4 NA) dry
  

9 Laser lines in the visible range:

• Violet 405nm (50mW), Blue Argon 458nm, 476nm, 488nm, 496nm, 514nm (65mW), Green DPSS 561nm (20mW), Orange HeNe 594nm (2mW), Red HeNe 633nm

Microscope enclosure with temperature, humidity and CO2 control
High precision Super Z motorized stage with multi-point and multi-tile soft-ware control. 3nm resolution galvometric z-axis control

U28-E12. FEI Vitrobot Mark IV

Specifications:
Working temperature 4 – 60 °C (at an ambient temperature range between 18 – 25 °C).
Peltier controlled heating/cooling.
Maintain relative humidity at 100 %.
Ultrasonic controlled humidification.
Linux operating system.
Touch screen control and set-up. Mouse and foot pedal controls also available.
Small sample volumes can be applied manually with a pipette through a small side port on the left and the right side of the climate chamber.
Both application time and wait time (between application and blotting) are software controlled and can be set in the user interface.
Precisely timed control of multiple sample applications, blotting actions, and vitrification enables time resolved analysis of interactions among separately applied components.
Excess fluid is removed from the grid by (repeated) blotting with filter paper on rotating foam pads.
Number of blotting actions (max. 16 times for one grid) and duration of blotting are software controlled and can be set in the user interface.
Longitudinal grid positioning (‘blot offset’) and wait time between blotting and vitrification (‘drain time’) are user definable.
Automated shutter control allows smooth, instant injection of the sample grid into the coolant (liquid ethane or propane). A lift for the container brings the coolant close to the shutter to ensure optimal vitrification.
Synchronous lowering of the coolant container and the grid holder keeps the grid submerged in the coolant and minimizes the risk of contamination prior to transfer ring the sample into a storage box or cryo holder.
Coolant container including an integrated anti-contamination ring.

U28-E11. Leica EM AFS2

Specifications:
Reproducible operations.
Excellent specimen visibility with internal LED illumination.
Working range from -140 °C to +70 °C.
“Deep Freeze” allows sample transfer at temperatures below -140 °C.
Transfer function “TF” excludes humidity and oxygen.
Fast UV polymerization with LED UV lamp.
Any choice of substitution system, e.g.:

• Substitution Capsule and Flat Embedding System.
• New Microtube Embedding System EM AFS2.
• Sapphire Disk FS System for cell cultures.
  Intuitive programming:
• 10 users.
• 99 programs each.
• 99 program steps each, 0 to 99 hrs 59 mins.
• Graphic display of substitution protocol.
• Menu prompts.

Mouse controlled color screen.
Memory stick to transfer programs.
Log file download on memory stick.
35 liters Dewar for liquid nitrogen.
Liquid nitrogen filling from outside specimen chamber, with a consumption enough for 5 day protocols.
Stainless steel working platform.
The Leica EM FSP (“Freeze Substitution Processor”) is an automatic reagent handling system. Mounted on the Leica EM AFS2, it dispenses reagents for both FS and PLT. It automatically dilutes FS media and resins from 100 % reagent containers. An integrated LED UV lamp allows immediate polymerization of the samples.

U28-E10. Leica EM HPM100

Specifications:
• Automatic freezing of samples with one button operation.
• Extremely high cooling rates.
• Perfect freezing of samples up to 6 mm diameter.
• No cryoprotectants required.
• Rapid transfer loading device for CLEM (‘Correlative Light and Electron Microscopy’) work.
• Fast, ergonomic sample loading.
• Express sample handling for fast freezing.
• Graphical data display of actual temperature, time and pressure for each run.
• Integrated work station.
• Integrated stereomicroscope and adjustable LED illumination.
• Universal and application specific sample carriers.
• Integrated Dewar with drain outlet.
• Automatic bake-out cycle.
• Thermally insulated process chamber.
• Integrated touch control screen with prompt window.
• Freezing process cover for ultimate safety.
• USB interface for data storage and transfer.
• Integrated air compressor.

U28-E09. Leica FC7 cryo-chamber on Leica EM UC7 ultramicrotome

Specifications:
• Using the touchscreen control, three different cryo-modes are available:
– Standard.
– High gas flow, increased gaseous nitrogen flow reduces ice contamination below -140 °C.
– Wet sectioning, to set a temperature difference of up to 130 °C between knife (-40 °C) and specimen (-170 °C), which is useful for, e.g., DMSO applications.

• Leica EM CRION ionizer with electrostatic charge and discharge functions controlled by foot switches greatly improves the method of cryosection collection.
• The attachable micromanipulator provides precise positioning of the grid to make section collection easier than ever before.
• Multiple lighting angles using the patented LED chamber illumination provide excellent visibility for section manipulation and pick up.
• Contact-free, through-the-wall specimen arm makes the instrument highly stable for chatter-free cryosectioning.
• Heated chamber walls prevent icing over a prolonged working time and enhance user comfort.
• Especially for frozen hydrated sectioning or the Tokuyasu method, the Leica EM CRION with electrostatic discharge and charge mode used with the micromanipulator provides outstanding cryo sectioning performance.

U28-E08. FEI Quanta 250 FEG Scanning Electron Microscope + ESEM Mode

Specifications:
Everhart-Thornley detector of secondary electrons for high vacuum applications providing topographical information
High contrast backscattered electron detector optimized to work with low volt-ages: vCD detector. Suitable for high-vacuum and low vacuum modes, provides topographical and compositional contrast
Scanning-transmission electron microscopy detector (STEM) for high vacuum applications. Allows to introduce 8 grids in one vacuum cycle and to perform bright field and dark field analysis
Large field detector (LFD) for low vacuum and ESEM applications. Allows working in SE and BSE modes and facilitates the observation of large fields of view
Gaseous secondary electrons detector (GSED) for environmental-SEM (ESEM) applications
Gaseous analytical detector (GAD) for ESEM applications
Wet scanning-transmission electron microscopy detector (Wet-STEM) for
ESEM applications

U28-E07. FEI Tecnai G2 20 TWIN Transmission Electron Microscope + Cryo-TEM

Specifications:
Electron source with LaB6 emitter and flexible high tension between 20 and 200 kV
Twin objective lens for high contrast and high tilt applications
Side-mounted CCD Olympus Veleta with 2k x 2k MP, high field of view and fast read-out rates for real time observation
Bottom mounted CCD FEI Eagle, 4k x 4k MP, HS scintillator
EDS thanks to EDAX DPP-II analyzer with Si (Li) detector
Low-dose control software mode for electron beam damage protection of the samples.
Xplore3D software for automated tomography control.
Inspect3D software for post alignment of tomograms.
Amira software for 3D reconstruction.
Sample holders:

• Single tilt
• Low background (for EDS applications) double tilt
• Fischione 2020 advanced tomography holder
• GATAN Tomography holder for samples at room temperature.
• GATAN Cryo-station for transferring of cryo-samples to the cryo-holder, previously vitrified of frozen with the high-pressure system.