High Resolution Liquid NMR
The NMR service is equipped with a High resolution NMR Spectrometers :a 400MHz (NMR spectrometer for routine experiments and a higher filed) y 600MHz (spectrometer dedicated NMR studies that are more demanding in terms of sensitivity)
Applications of NMR spectroscopy to chemistry range from structure elucidation of small molecules to studies of ligand-protein interactions. In biomedicine, the use of NMR spectroscopy has expanded from the classical metabolic studies using enriched stable isotope tracers to the more recent NMR-based metabolomics of biofluids (urine, plasma, CSF).
Customer benefits
We have SOPs and ISO9001 certification. We also have specialist technicians for the use of the equipment.
This service is essential to:
- Structure verification and elucidation
- Molecular mobility: determination of conformation in solution
- Determination of enantiomeric excess
- Kinetic and temperature studies of reaction mixtures
- Metabolomic analysis of biofluids (urine, blood serum, etc.)
Target customer
Any company or research group interested in:
- Acquisition of 1D NMR spectra at 400 MHz and 600MHz (1 H, 13C, 31P, etc.)
- Acquisition of basic 2D experiments: COSY, TOCSY, HSQC, HMBC, J-RES
- Nuclear Overhauser Effect experiments (NOE and ROE), Diffusion experiments (DOSY), selective excitation experiments.
- Preparation of biofluid samples (urine, blood serum/plasma) for high resolution NMR spectroscopy following standardized protocols.
- Basic processing of NMR spectra.
References
- Pomary PK, Eichau S, Amigó N, Barrios L, Matesanz F, García-Valdecasas M, Hrom I, García Sánchez MI, Garcia-Martin ML. Multifaceted Analysis of Cerebrospinal Fluid and Serum from Progressive Multiple Sclerosis Patients: Potential Role of Vitamin C and Metal Ion Imbalance in the Divergence of Primary Progressive Multiple Sclerosis and Secondary Progressive Multiple Sclerosis. J Proteome Res. 2023 Mar 3;22(3):743-757. doi: 10.1021/acs.jproteome.2c00460. Epub 2023 Jan 31. PMID: 36720471; PMCID: PMC9990127.
- Molina N, Martin-Serrano A, Fernandez TD, Tesfaye A, Najera F, Torres MJ, Mayorga C, Vida Y, Montañez MI, Perez-Inestrosa E. Dendrimeric Antigens for Drug Allergy Diagnosis: A New Approach for Basophil Activation Tests. Molecules. 2018 Apr 24;23(5):997. doi: 10.3390/molecules23050997. PMID: 29695102; PMCID: PMC6100007.
- Fernández-Palacios S, Matamoros E, Morato Rojas I, López Navarrete JT, Ruiz Delgado MC, Vida Y, Perez-Inestrosa E. New Insights into Acylhydrazones E/Z Isomerization: An Experimental and Theoretical Approach. Int J Mol Sci. 2023 Sep 29;24(19):14739. doi: 10.3390/ijms241914739. PMID: 37834186; PMCID: PMC10648745.