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U28-E06. Bruker Minispec MQ-60

U28-E06. Bruker Minispec MQ-60

The Minispec TD-NMR is broadly used in research, development and quality control of MRI Contrast Agents. These agents are used to enhance contrast in Magnetic Resonance images between tissues that otherwise would be difficult to differentiate. Most of these contrast agents are based on Gd chelates or iron oxide nanoparticles, which produce signal contrast enhancement by affecting the magnetic relaxation properties of water molecules. The Minispec MQ-60 provides T1 and T2 measurements with high accuracy and reproducibility, and, most importantly, at clinical field, which is fundamental for translational research. Hence, the Minispec MQ-60 plays a key role in the characterization of new contrast agents for clinical use.

Specifications:
Permanent 1.4T magnetic field.
“Intelligent” 1H probe (“plug and play”) with 60 MHz operating frequency
Fast magnet warm-up and precise digital temperature regulation for ultimate results stability
Optical detector to any kind of glass, quartz and plastic samples. Specific filters for natural and fluorescent light suppression and energy saving lamps

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U28-E05. Bruker Avance 600 MHz NMR spectrometer + HR-MAS Probe

U28-E05. Bruker Avance 600 MHz NMR spectrometer + HR-MAS Probe

Specifications:
UltraShieldTM Plus 14.1 T magnet
5 mm BBFOPLUS probe, optimized for X-nuclei direct observation in a frequency range between 31P and 15N. Automated tuning and matching (ATM).
5 mm TXI probe, optimized for higher sensitivity 1H-nuclei observation. The outer coil is optimized for decoupling of 13C and 31P.Automated tuning and matching device (ATM).
TCI CryoProbe Prodigy, uses nitrogen-cooled RF coils and preamplifiers for 1H, 2H and 13C, delivering a 2.5x 1H sensitivity boost. The probe is ATM compatible and has 2H “lock” channel and Z-gradient.
Control temperature unit (from 0 ºC to 50 ºC)

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U28-E04. Bruker Ascend 400 MHz NMR spectrometer

U28-E04. Bruker Ascend 400 MHz NMR spectrometer

Specifications:
UltraShieldTM Plus 9.4T magnet
5 mm BBFOPLUS probe, optimized for X-nuclei direct observation. Broad band in a frequency range between 31P and 15N. This probe has 2H “lock” channel and z gradient that allows 2D spectroscopy and hetero correlation 19F/1H and 1H/19F, to be performed. It also includes an automated tuning and matching device (ATM)
Sample Xpress Lite (for 16 samples)
Control temperature unit (from 0 ºC to 50 ºC)

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U28-E03. Bruker Albira small animal CT system

U28-E03. Bruker Albira small animal CT system

Specifications:
Dedicated beds for individual rats and mice with integrated gas anesteasia.
Albira Software Suite 5.0 with integrated acquisition and tomography software.
PMOD biomedical image quantification software.
X-ray Focal Spot Size (Nominal) 35 μm.
Energy Range (kVp) 10-50.
Field of View (FOV) (transaxial x axial) 70 mm x 70mm
Multiple FOV windows can be combined for whole animal imaging
Bore Size 105 mm

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U28-E02. Bruker In vivo Xtreme

The In-Vivo Xtreme is a multi-modal optical and X-ray small animal imaging system, designed to combine sensitivity, speed and flexibility for the broadest range of applications for molecular imaging. Equipped with a high performance f/1.3 58mm lens and 4MP back-illuminated CCD camera, the In Vivo Xtreme is suitable for demanding bio-luminescence and fluorescence intravital imaging challenges

Specifications:
Back-illuminated 4 MP camera
f/1.1 – f/16, 58 mm fixed lens (on movable platform)
400W Xenon Light Source
28 Excitation Filters 410 nm – 760 nm
Fluorescent imaging from the visible to near IR
Imaging chamber for up to five mice at a time with
integrated anesthesia and temperature control
Automated switching between modalities – the animal never moves
Multimodal Animal Rotation System (MARS)
Integrated high resolution X-ray imaging (>18lp/mm spot size <60μm)
X-ray illumination optimized for small animal imaging with 5 selectable filter settings for fine tuning

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U28-E01. Bruker BioSpec 9.4T animal MRI system

U28-E01. Bruker BioSpec 9.4T animal MRI system


The Biospec 94/20 is a multipurpose research system for high-resolution magnetic resonance spectroscopy and imaging. The system is designed for preclinical and molecular MR imaging. State-of-the-art RF probes technology together with ultra high field USR magnets deliver high spatial resolution in vivo enabling researchers to come closer to the molecular and cellular level (molecular imaging). Thanks to its innovative modular concept, virtually any small animal MR imaging application in life science, biomedical and preclinical research can be conducted.
Specifications:
Magnetic field strength of 9.4T
20 cm bore diameter, suitable for mice and rats
Automated positioning system (AutoPac™)
High performance actively shielded
gradient system with integrated shims
RF coils for rats and mice

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U28-S021. Nuclear Molecular Imaging (PET/SPECT/CT)(Onsite&Remote)


Applications:

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U28-S020. High Content Screening

High Content Screening

High content screening (HCS) attempts to bridge the gap between microscopy and cytometry, providing both large quantities of statically useful and phenotypically rich data. HCS combines automated microscopy with customizable algorithms able to detect and quantify many different types of phenotypic information in both fixed and living cultured cells. The Perkin Elmer Operetta HCS system combines automated imaging with advanced Harmony HCS software, which contains many common algorithms such as nuclei and cell detection, as well as the ability to automatically detect complex phenotypes through machine learning.

Customer benefits

We have SOPs and ISO9001 certification. We also have specialist technicians for the use of the equipment.

This service is essential to:

  • Fast and statistically robust toxicity assays using adherent cells with the advantage of being able to visually confirm cell morphology.
  • Drug dose-response curve calculation: On a single plate, multiple concentrations or combinations of compounds can be easily and quickly tested.
  • Screening for specific phenotypes in cultured cells using libraries of small molecules, siRNAs, or other biologically-active collections.
  • Screening for complex phenotypes: many cytological effects cannot be detected easily by cytometry Operetta allows complex phenotypes (eg organelle distribution or cell organization changes) to be detected automatically and reliably.
  • Long-term cell culture studies: Operetta is equipped with temperature and CO2 control, making it suitable for maintaining live cultured cells in optimal conditions during image acquisition.

Target customer

Any company or research group interested in:

  • High content screening design and setup advice The most important step in performing a successful HCS screen is good experimental design and planning.
  • Experimental validation and pilot study testing For more complex projects, it is essential to perform preliminary tests to identify possible problems with labeling, marker expression, cell morphology, and other sources of variability before expending valuable time and reagents.
  • Custom experiments and HCS analysis design: we can design both assays and custom automatic image analysis pipelines using the Harmony platform based on your requirements.
  • Integrated cytotoxicity testing service We have developed robust protocols from the incubation of cultured cells with test compounds to measuring cytotoxic responses at different time points Different criteria such as plasma membrane integrity, metabolic activity, or intracellular esterase activity can be chosen depending on experimental requirements.

References

  1. E. Gómez-González, C. Caro, M.L. García-Martín, A.I. Becerro,* M Ocaña. Outstanding MRI contrast with Dysprosium phosphate Nanoparticles of tuneable size. Nanoscale 2022, 14, 11461-11470. doi: 10.1039/D2NR02630A
  2. Caro C, Guzzi C, Moral-Sánchez I, Urbano-Gámez JD, Beltrán AM, García-Martín ML. Smart Design of ZnFe and ZnFe@Fe Nanoparticles for MRI-Tracked Magnetic Hyperthermia Therapy: Challenging Classical Theories of Nanoparticles Growth and Nanomagnetism. Adv Healthc Mater. 2024 Feb 2:e2304044. doi: 10.1002/adhm.202304044. Epub ahead of print. PMID: 38303644.

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U28-S019. Fluorescence microscopy

Fluorescence microscopy

Conventional widefield fluorescence microscopy is still the best choice for many applications. The CCD and CMOS-based sensors used for conventional microscopy are typically much more sensitive than the photomultiplier tubes used in confocal microscopes and flow cytometers. As the camera captures the whole field of view at the same time, it also allows for faster imaging in many cases. Examples where conventional microscopy may be advantageous include the visualization of individual molecules, receptors, or small organisms such as bacteria and yeast.

Customer benefits

We have SOPs and ISO9001 certification. We also have specialist technicians for the use of the equipment.

This service is essential to:

  • Nanoscale imaging of fixed samples
  • Dynamic imaging of cytoskeletal structures, focal adhesion formation, as well as endocytosis and vesicle dynamics in live cells.
  • Fluorescent analysis of histological samples.

Target customer

Any company or research group interested in:

  • Long-term (>4 days) imaging of bacterial colonies and bacterial interactions using DIC, temperature control, autofocus, and multi-field imaging.
  • Routine multi-color fluorescent analysis of histological samples where confocal or other more advanced methods are not necessary.
  • Imaging of bone, muscle, or connective tissues in histological samples using circularly-polarised light.

References

Carrillo P, Bernal M, Téllez-Quijorna C, Marrero AD, Vidal I, Castilla L, Caro C, Domínguez A, García-Martín ML, Quesada AR, Medina MA, Martínez-Poveda B. The synthetic molecule stauprimide impairs cell growth and migration in triple-negative breast cancer. Biomed Pharmacother. 2023 Feb;158:114070. doi: 10.1016/j.biopha.2022.114070. Epub 2022 Dec 14. PMID: 36526536.

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U28-S018. Super-resolution microscopy

Super-resolution microscopy

Total Internal Reflection Fluorescence (or TIRF) is a powerful technique that combines the sensitivity of conventional fluorescence with selective illumination to improve the contrast of features very close to the sample coverslip. TIRF is often used for studies related to membrane dynamics, receptor-ligand interactions, and vesicular transport.
The intrinsic diffraction of light has historically made it difficult to use fluorescence to distinguish structures closer than 200nm apart. Super-resolution microscopy refers to techniques that selectively activate fluorescent molecules to map their position with up to 10 times more accuracy than conventional fluorescent microscopy. The Nikon N-Storm system is capable of localizing molecules with a resolution of up to 20nm and is compatible with all most current localization protocols and fluorophores (including Alexafluor 647 and Atto488).

Customer benefits

We have SOPs and ISO9001 certification. We also have specialist technicians for the use of the equipment.

This service is essential to:

  • Nanoscale imaging of fixed samples
  • Dynamic imaging of cytoskeletal structures, focal adhesion formation, as well as endocytosis and vesicle dynamics in live cells.
  • Single-molecule studies and localization microscopy modalities including N-STORM, Direct STORM, and PALM.
  • Fluorescent analysis of histological samples.

Target customer

Any company or research group interested in:

  • STORM image processing: we can convert localization imaging data into SR images using both proprietary and open source software packages.
  • TIRF imaging of membrane and/or cytoskeletal dynamics in live adherent cells using commercial fluorescent labels such as DiR, FM4-64, and Cell-Light or user-supplied reagents.
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