2017 - Nanbiosis

U14-S05. Determination of cytokine concentration by flow cytometry

Determination of cytokine concentration by flow cytometry

The determination of cytokine concentration by flow cytometry service represents a cutting-edge approach to cytokine analysis within the realm of biomedical research and pharmaceutical investigation. Through the utilization of advanced flow cytometry techniques and specialized staining protocols, this service enables the precise quantification of intracellular cytokines across distinct cell subsets distinguished by surface markers. This service facilitates the elucidation of cytokine profiles within specific cell populations, shedding light on their roles in health, disease, and therapeutic interventions.

Customer benefits

U14 Cell Therapy Unit offers researchers precision, efficiency, and expertise, streamlining experiment. With stringent quality control, ISO 9001-2015 and GLP compliant, tailored solutions, and access to advanced equipment, researchers can focus on innovation and core objectives, driving scientific progress forward. By delving into the intracellular milieu, researchers gain invaluable insights into the intricate regulatory networks governing immune responses and cellular signaling pathways.

Target customer

This service is essential for organizations involved in Research and Development, including pharmaceutical companies, academic institutions for biomedical research, biotechnology firms for bioprocessing, and regulatory agencies for in vitro studies.

Additional information

Selected references:

de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther. 2023. Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551. https://doi.org/10.1186/s10020-023-00646-1
Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM. 2022. Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells Animals. MDPI. 12-20. ISSN 2076-2615. https://doi.org/10.3390/ani12202798
Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther. 2021. A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10). 2021. IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. MDPI. 22-22. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222212177
López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269. https://doi.org/10.1007/s12015-019-09926-y

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U14-S04. Cytokine quantification assays

Cytokine quantification assays

The cytokine quantification assays service is at the forefront of cytokine analysis in biomedical research and pharmaceutical development. Leveraging advanced immunoassay techniques and specialized expertise, this service offers precise quantification of cytokines present in cell culture supernatants or animal fluids. Through meticulous analysis, researchers gain valuable insights into the intricate signaling networks and immune responses underlying various physiological and pathological processes. From characterizing inflammatory cascades to evaluating therapeutic interventions, our service provides a robust platform for investigating cytokine dynamics and their implications for health and disease.

Customer benefits

Target customer

Additional information

Selected references:

de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther. 2023. Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551. https://doi.org/10.1186/s10020-023-00646-1
Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM. 2022. Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells Animals. MDPI. 12-20. ISSN 2076-2615. https://doi.org/10.3390/ani12202798
Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther. 2021. A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10). 2021. IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. MDPI. 22-22. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222212177
López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269. https://doi.org/10.1007/s12015-019-09926-y

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U11-S2. Formulation Lab

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Nanomedicine Summer School

The second Summer School in Nanomedicine coordinated by Simó Schwartz, Scientific Director of NANBIOSIS unit 20, was held at Vall d’Hebron hospital on 28 and 29 September. Dr. Schwartz has been the president of the European Society for Nanomedicine (ESNAM), a European scientific society focused on the clinical application of nanomedicine. From this society tasks are carried out to promote the research and the use of nanomedicine in the health sector, as well as to facilitate the exchange of knowledge in this area at European level among all the actors involved, Academy, industry and entities regulators.

CIBER-BBN, partner of NANBIOSIS, as sponsor of ESNAM, has collaborated in the organization of this event. Lectures were given by some Scientific Directors or Coordinators of NANBIOSIS units as Jaume Veciana, Ibane Abasolo, Laura Lechuga and Antoni Villaverde .

Photos of the meeting can be viewed in this link.

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U26-S04. In vivo NMR of small animals (zebrafish,Drosophila)

In vivo NMR of small animals (zebrafish, Drosophila)

The service is intended for obtaining images of small animals such as zebrafish, Drosophila. Also longitudinal studies can be performed. Thanks to a 14 T NMR equipment equipped with a micro-image probe.

Customer benefits

The service is integrated in the University of Valencia core facility that ensures the correct maintenance and the offsite service runed by technicians under ISO 9001.

Target customer

The primary audience are pre-clinic research groups that require punctual or longitudinal studies on such small animals with high resolutions.

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U20-S11. Histological porcessing, section and staining (Remote) OUTSTANDING

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U20-S09. X-ray imaging in vitro and in vivo (Remote) OUTSTANDING

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U13-S08. Atomic Force Microscopy indentation test

Atomic Force Microscopy indentation test

Atomic Force Microscopy can be used to measure the forces between the probe and the sample as a function of their mutual separation. This can be applied to perform force spectroscopy.

Customer benefits

Low forces on cells (nano-scale).

Target customer

Public and private research groups focused on mechanobiology

Additional information

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U10-S9.

Characterization and development of pulmonar formulations

Encapsulation of therapeutic actives of interest in formulations for pulmonary administration with possibility of lyophilisation to obtain a powder. The service has two cutting-edge equipments for the characterization of pulmonary formulations:
SprayTec laser diffraction system: allows the measurement of spray particle and spray droplet size distributions in real-time for more efficient product development of sprays and aerosols, with robust and reproducible droplet size data.
Next Generation Impactor: has been designed specifically for the pharmaceutical industry for testing metered-dose inhalers, drypowder inhalers, nebulizers and nasal sprays. It consists of a high performance cascade impactor for classifying aerosol particles into micrometer size fractions, providing relevant information about their distribution in the respiratory tract.

Customer benefits

The formulations can be characterized, following SOPs, in terms of particle size, polydisperstity index and zeta potential. Importantly, this service can also offer real-time droplet size distribution and aerosol particles classification analysis by means of SprayTec and Next Generation Impactor (NGI) technology.

Target customer

Preclinical use for the characterization of the pulmonary formulation prior to its implementation in in vivo models.
Companies interested in characterizing their formulations or their pulmonary administration systems.

References

Moreno-Sastre M, Pastor M, Esquisabel A, Sans E, Viñas M, Fleischer A, Palomino E, Bachiller D, Pedraz JL. Pulmonary delivery of tobramycin-loaded nanostructured lipid carriers for Pseudomonas aeruginosa infections associated with cystic fibrosis. Int J Pharm. 2016 Feb 10;498(1-2):263-73. doi: 10.1016/j.ijpharm.2015.12.028.

Additional information

U10 Pulmonar formulations characterization.tif

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U10-S8.

3D bio-impression of scaffolding for regenerative medicine

The principle of 3D bioprinting consists of selecting the most suitable biomaterials and cell types to prepare a Bioink that should be able to promote cell growth and differentiation and present appropriate mechanical properties of the target tissue.
This service possess a wide variety of 3D bioprinting techniques avaliable, such as extrusion, droplet, electrospining, electrowritting and stereolithography.

Customer benefits

One of the main characteristics of this additive manufacturing technique is its ability to bioprint the desired layers, with specific cell orientation , and desired morphology of the bioprinted 3D scaffold in order to ressemble, as much as possible, the tissue of interest. To achieve this goal, rheology, texturometry, printability and biological assays are carried out.

On the one hand, this technology can be employed to develop 3D scaffolds specific for the regeneration of particular tissues. On the other hand, this strategy offers a 3D environment that mimics the tissue/ organ of interest in order to test potential therapeutic tools, which goes in accordance with the implementation of the 3R principle (replace, reduce and refine).

Target customer

Preclinical use for in vitro and in vivo models.
Pharmaceutical industry (e.g. cosmetics)

References

Lafuente-Merchan M, Ruiz-Alonso S, García-Villén F, Zabala A, de Retana AMO, Gallego I, Saenz-Del-Burgo L, Pedraz JL. 3D Bioprinted Hydroxyapatite or Graphene Oxide Containing Nanocellulose-Based Scaffolds for Bone Regeneration. Macromol Biosci. 2022 Nov;22(11):e2200236. doi: 10.1002/mabi.202200236.
Lafuente-Merchan M, Ruiz-Alonso S, Zabala A, Gálvez-Martín P, Marchal JA, Vázquez-Lasa B, Gallego I, Saenz-Del-Burgo L, Pedraz JL. Chondroitin and Dermatan Sulfate Bioinks for 3D Bioprinting and Cartilage Regeneration. Macromol Biosci. 2022 Mar;22(3):e2100435. doi: 10.1002/mabi.202100435.
Ruiz-Alonso S, Villate-Beitia I, Gallego I, Lafuente-Merchan M, Puras G, Saenz-Del-Burgo L, Pedraz JL. Current Insights Into 3D Bioprinting: An Advanced Approach for Eye Tissue Regeneration. Pharmaceutics. 2021 Feb 26;13(3):308. doi: 10.3390/pharmaceutics13030308.

Additional information

U10 Biorpinters.png 3D Bioprinters: BIO X 3D Bioprinter –CELLINK (left); R-GEN 100 –REGENHU (right).

Bioprinted 3D scaffold

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Nanbiosis

You are here : Home / 2017 (Page 5)

Determination of cytokine concentration by flow cytometry

Cytokine quantification assays

In vivo NMR of small animals (zebrafish, Drosophila)

Atomic Force Microscopy indentation test

Characterization and development of pulmonar formulations

3D bio-impression of scaffolding for regenerative medicine