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Nanbiosis

U19-E20. ABL80 FLEX Gasometer Analyzer (RADIOMETER)

ABL80 FLEX Gasometer Analyzer (RADIOMETER)

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U19-E19. Veterinary Hematology Analyzer (MINDRAY BC-5300VET)

Veterinary Hematology Analyzer (MINDRAY BC-5300VET)

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U10-S09. Characterization and development of pulmonar formulations (On-site&Remote) OUTSTANDING

Characterization and development of pulmonar formulations

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U10-S08. 3D bio-impression of scaffolding for regenerative medicine (On-site&Remote) OUTSTANDING

3D bio-impression of scaffolding for regenerative medicine

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NANBIOSIS presented at the 12th annual event of the ETPN

During the days 17-19 of October Malaga has hosted the 12th annual event of European Platform of Technology in Nanomedicine (ETPN), a European meeting of 200 experts in research and innovation in nanomedicine.

The meeting was organized by Bionand, the Spanish Platform of Nanomedicine (Nanomed Spain), the Institute of Nanoscience of Madrid (Imdea) and the Center for Biomedical Research Network in the area of ​​Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), partner of NANBIOSIS.

During the event, the ETPN General Assembly took place as well as parallel meetings of the specialized working groups on different scientific and cross-cutting aspects related to nanomedicine

In this framework, the ICTS NANBIOSIS has been presented by the Scientific Director of CIBER-BBN, Ramón Martínez Máñez, to the European Nanomedicine community.

EUROPEAN TECHNICAL PLATFORM OF NANOMEDICINE

The European Technology Platforms are a key element in the European field of innovation, associations born with the mission of sharing knowledge, mobilizing the actors involved and developing sector strategies, all based on the social challenges established by the European Commission and with the ultimate goal of its translation to the market.

The European Nanomedicine Technology Platform (ETPN) was created in 2005 by the European Commission and a group of experts from academia and industry who have since contributed to the publication of various strategic documents identifying the needs and the roadmaps for research in Nanomedicine in Europe. Its main objectives are to establish a clear strategic vision of the sector, to reduce the fragmentation of nanomedical research, to mobilize public and private investment, to identify priority areas, to promote innovation in nanobiotechnologies for medical use and to strengthen Europe’s competitiveness in this sector scientific and industrial level

Currently, the ETPN has more than 120 members from 25 countries, representing all actors involved in nanomedicine, such as academic institutions, research centers, small and medium enterprises, industry, public agencies, representatives of the platforms the European Commission itself, among others.

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U23-S09. Quality control for Human Assisted Reproduction clinics

Quality control for Human Assisted Reproduction clinics

The assisted reproduction unit has a laboratory with extensive equipment with which to carry out the basic assisted reproduction techniques, among which are extraction of gametes (ovum and sperm), extraction of embryos, sperm analysis, vitrification and devitrification of gametes and embryos, in vitro fertilization (IVF) and ICSI. It also has a small animal surgery to perform surgery on small animals, mainly mice, which is the animal species with which we preferably work. To carry out these techniques, the Assisted Reproduction Unit is equipped with two intracytoplasmic micromanipulation equipment with IMSI, Laser and Oosight systems, embryo biopsy systems, and mitotic spindle vision systems. We also have a piezoelectric device to carry out ICSI with oocytes from different animal species. We have recently incorporated a Time Lapse incubator to evaluate embryonic development; we also have flow cabinets with stereomicroscopes and heaters, plates, incubators with different gas systems, freezing and cryopreservation equipment for gametes and embryos, among others.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out quality control for Human Reproduction clinics, studies of in vitro fertilization and viability of embryo culture media, Micromanipulation and microinjection techniques of mouse embryos or other animal species, whether that we have piezo electric. Furthermore, as we have a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

  • Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
    British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
  • Optimizations of an ovum pick up protocol in Donkey.
    Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
    https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
  • Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
  • Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
    Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Reproduction. Fertility and Development Sep;34(15):980-990
    https://doi.org/10.1071/RD22041
  • 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
    Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
    https://doi.org/10.1093/molehr/gaaa075
  • Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
    Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
    Methods X. vol 7, pp:1-9; 2020, 100767
    https://doi.org/10.1016/j.mex.2019.12.008
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U23-S08. Oosight system

Oosight system

In addition to having micromanipulation and microinjection equipment that allows us to inject sperm into the cytoplasmic cavity of the oocyte (ICSI). One of these microinjectors has a built-in Oosight system implemented which allows us to non-invasively (without adding dyes) precisely observe the location of the mitotic spindle indicative of the correct development and optimal degree of maturation of the oocyte. This facilitates the selection of the most suitable oocyte for the ICSI or IMSI procedure. In addition, Oosight IS indicates the insertion site of the sperm inside the oocyte so as not to damage sensitive cytoplasmic structures.

Customer benefits

Having these micromanipulation and microinjection equipment that incorporates the Oosight system to evaluate the quality and degree of maturation of the oocytes allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out carry out this Technique in the human clinic. Furthermore, the implementation of this oosight imaging system allows them to become familiar with the internal structures of the oocyte and classify it according to its level of development, learning to differentiate which are the most optimal oocytes to later perform ICSI or IMSI. This equipment and the oosight system
It allows the practical training of students of master’s degrees and specialization courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out on animal species, our laboratory is suitable for it. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance and service to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process. without damaging the oocyte. In addition, they can carry out studies that allow visualization of the internal structures and location of the mitotic spindle, which will allow them to select those oocytes that present the best morphology and are fully mature to be fertilized later.

Additional information

  • Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
    British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
  • Optimizations of an ovum pick up protocol in Donkey. Abstract published in: Anim Reprod. 2022;19(2 spe):e22177
    https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
  • Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio. Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
  • Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio. Reproduction. Fertility and Development Sep;34(15):980-990
    https://doi.org/10.1071/RD22041
  • 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS. Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075. https://doi.org/10.1093/molehr/gaaa075
  • Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts. Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc Methods X. vol 7, pp:1-9; 2020, 100767 https://doi.org/10.1016/j.mex.2019.12.008
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U23-S07. Laser System and Biopsed of blastomeres

Laser System and Biopsed of blastomeres

In addition to having micromanipulation and microinjection equipment that allows us to inject sperm into the cytoplasmic cavity of the oocyte (ICSI). One of these microinjectors has a built-in laser system, which allows, by means of small shots, to make a small hole in the membrane of the embryo and, after absorption with specific needles for this process, obtain two or three cells of the trophectoderm and subsequently send these cells to specialized laboratories that perform preimplantation genetic diagnosis studies, to analyze possible abnormalities of the embryo before its transfer and implantation.

Customer benefits

Having these micromanipulation and microinjection equipment that incorporates a laser system to perform trophectoderm biopsy allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out this technique in the human clinic. Furthermore, the implementation of this ICSI technique and blastomere biopsy, and the handling and use of micromanipulators and microinjectors allows the practical training of master’s students and specialization courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out with animal species, our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process without damaging the oocyte In addition, they can perform a biopsy of the embryo’s blastomeres in order to later perform genetic studies on it.

Additional information

  • Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
    British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
  • Optimizations of an ovum pick up protocol in Donkey.
    Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
    https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
  • Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
  • Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
    Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Reproduction. Fertility and Development Sep;34(15):980-990
    https://doi.org/10.1071/RD22041
  • 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
    Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
    https://doi.org/10.1093/molehr/gaaa075
  • Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
    Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
    Methods X. vol 7, pp:1-9; 2020, 100767
    https://doi.org/10.1016/j.mex.2019.12.008
Read More

U23-S06. IMSI System

IMSI System

Within the Assisted Reproduction Unit we have a laboratory equipped with two micromanipulation and microinjection equipment that allows us to inject the sperm into the cytoplasmic cavity of the oocyte (ICSI), in order to carry out fertilization. The advantage that this equipment offers is that it allows us to perform in vitro fertilization without having to have a large number of sperm and oocytes. Furthermore, to be able to perform this technique with oocytes from different animal species, we have a piezoelectric device to perform this intracytoplasmic injection without damaging the oocyte of the specific animal species. Another possibility offered by this service is that in one of these microscopes we have a 6000x objective, which allows us to see the sperm in large sizes. In this way we can select the sperm that are morphologically of the best quality for intracytoplasmic injection (IMSI), which will subsequently allow us to obtain better quality embryos.

Customer benefits

Having these micromanipulation and microinjection equipment allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out this technique in the human clinic. In addition, the implementation of this ICSI and IMSI technique and the handling and use of micromanipulators and microinjectors allows for practical training for master’s students and specialized courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out with animal species, so our laboratory is suitable for it. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process without damaging the oocyte.

Additional information

  • Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
    British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
  • Optimizations of an ovum pick up protocol in Donkey.
    Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
    https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
  • Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
  • Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
    Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Reproduction. Fertility and Development Sep;34(15):980-990
    https://doi.org/10.1071/RD22041
  • 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
    Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
    https://doi.org/10.1093/molehr/gaaa075
  • Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
    Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
    Methods X. vol 7, pp:1-9; 2020, 100767
    https://doi.org/10.1016/j.mex.2019.12.008
Read More

U23-S05. In vitro maturation

In vitro maturation

In the assisted reproduction unit we work with experimental animal models, mainly mice, in which we usually extract gametes and embryos, in which we evaluate the maturation process and viability of these oocytes, through tests with different culture media and with enrichment of these culture media with different compounds, to observe which of them produces the best degree of maturation, development and viability of the same, not only of oocytes but also of follicles from other animal species such as sheep. This development and duration can be observed directly using the time-lapse incubator and with different viability tests that are carried out later.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out studies of in vitro fertilization and viability of embryonic culture media for mouse embryos or other animal species, and the development and degree of maturation of oocytes and follicles after the addition of different compounds. to these culture media. Furthermore, by having a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

  • Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
    British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
  • Optimizations of an ovum pick up protocol in Donkey.
    Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
    https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
  • Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
  • Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
    Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Reproduction. Fertility and Development Sep;34(15):980-990
    https://doi.org/10.1071/RD22041
  • 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
    Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
    https://doi.org/10.1093/molehr/gaaa075
  • Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
    Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
    Methods X. vol 7, pp:1-9; 2020, 100767
    https://doi.org/10.1016/j.mex.2019.12.008
Read More