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U22-S03. In vivo toxicology in rodent and non-rodent species

In vivo toxicology in rodent and non-rodent species

We offer a specialized service of in vivo Toxicology in both Rodent and Non-Rodent species to evaluate possible toxicological effects of chemical, pharmaceutical and biological compounds. Our veterinarians guarantee and ethical and safe environment, performing a wide variety of standard and customized tests, such as acute, subchronic, chronic, reproductive, developmental, toxicity and mutagenicity studies. We work according to rigorous ethical and regulatory standards to generate reliable data essential for risk assessment in product development.We work with researchers to design and develop animal models, tailored to their specific needs. From the selection of the right animal to the methodology of disease induction, we ensure to create relevant and reliable models.

Customer benefits

All areas and facilities integrated in the unit have obtained certifications according to ISO-9001 and Good Laboratory Practices (GLP) standards, guaranteeing the production of highly accurate results.
This allows us to carry out preclinical research and safety and efficacy studies in animal models in full compliance with the rigorous criteria established by regulatory agencies, ensuring the reliability and traceability of all results and tests performed in the different services that we offer.

Target customer

  • Agrochemical companies.
  • Food and beverage industry.
  • Chemical manufacturers.
  • Academic institutions.
  • Regulatory agencies.
  • Pharmaceutical industry.
  • Any organization involved in the development, manufacture or products’ regulation with potential risks to human, animal or environmental health.

Additional information

Selected publications:

  • Soria F, Delacruz JE, Aznar-Cervantes SD, Aranda J, Martínez-Pla L, Cepeda M, Pérez-Lanzac A, Bueno G, Sánchez-Margallo FM. Animal model assessment of a new design for a coated mitomycin-eluting biodegradable ureteral stent for intracavitary instillation as an adjuvant therapy in upper urothelial carcinoma. Minerva Urol Nephrol. 2023 Apr;75(2):194-202.
  • Soria F, Morcillo E, López de Alda A, Pastor T, Sánchez-Margallo FM. Catéteres y stents urinarios biodegradables. ¿Para cuándo? [Biodegradable catheters and urinary stents. When?]. Arch Esp Urol. 2016 Oct;69(8):553-564.
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U22-S02. In vivo experimental design/consultancy for preclinal studies

In vivo experimental design/consultancy for preclinal studies

Committed to excellence in preclinical research, we offer complete services on in vivo experimental design. We strive to facilitate the advancement of your research on an ethically and efficient way, wether in person or remotely. We work closely with researchers to maximise the quality of the data and minimize resource usage, always prioritizing animal welfare. Our facilities feature state of the art animal housing designed to ensure the comfort and safety of animals used in preclinical studies. Our services include guidance in animal model selection, personalized experimental design, ethical and regulatory supervision of in vivo procedures, and detailed data analysis to support result interpretation.

Customer benefits

Quality Certificate from AENOR (ER-0430/2002) according to UNE-EN-ISO 9001:2015.
Certificate of Compliance with Good Laboratory Practices (BPLI 23.05/001 AEMPS) to conduct studies in both human and veterinary medicine, including:

  • In vivo toxicity.
  • Tolerance.
  • Pharmacodynamics.
  • Administration of investigational product and collection of non-clinical specimens.
  • Biocompatibility studies of medical devices.

We also have an associated Animal Care and Use Committee (Authorized body by the Government of Extremadura)

Target customer

  • Academic researchers.
  • Pharmaceutical and biotechnology companies.
  • Medical research centres.
  • Regulatory agencies and governmental organizations.
  • Clinical researchers.

Additional information

Selected publications:

  • Ballestín A, Casado JG, Abellán E, Vela FJ, Campos JL, Martínez-Chacón G, Bote J, Blázquez R, Sánchez-Margallo FM. A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery. J Vis Exp. 2019 Nov 8;(153).
  • Blázquez R, Álvarez V, Antequera-Barroso JA, Báez-Díaz C, Blanco V, Maestre J, Moreno-Lobato B, López E, Marinaro F, Casado JG, Crisóstomo V, Sánchez-Margallo FM. Altered hematological, biochemical and immunological parameters as predictive biomarkers of severity in experimental myocardial infarction. Vet Immunol Immunopathol. 2018 Nov;205:49-57. 
  • Sun F, Báez-Díaz C, Sánchez-Margallo FM. Canine prostate models in preclinical studies of minimally invasive interventions: part II, benign prostatic hyperplasia models. Transl Androl Urol. 2017 Jun;6(3):547-555.
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U19-S03. Clinical Analysis Laboratory

Clinical Analysis Laboratory

The clinical analysis laboratory that performs a wide variety of analysis: (biochemistry, hematology, coagulation, urine analysis, blood gas analysis, different biomarkers, hormones, allergens) in various animal species (rat, mouse, rabbit, pig, sheep, dog, and cat). Specific methodologies are used in each animal species, having all techniques validated according to their physiological and pathological identity. It also has a formulation laboratory equipped to formulate, reconstitute and guarantee the correct storage of the test products used in these studies.

Customer benefits

The laboratories are certified with ISO-9001 and Good Laboratory Practices (GLP), strict quality standards that allow the production of high-precision results.
Also, the studies can be performed under regulatory requirements too, since the performing institution is Certified for Good Laboratory Practices.
Therefore, studies can be carried out in compliance with the strict guidelines of regulatory agencies, ensuring the reliability and traceability of all results and tests carried out in their different services.

Target customer

The services offered in this unit may be of interest to different companies and laboratories that work within the pharmaceutical industry. Companies whose objective is to test possible candidates for molecules, drugs or medical devices.
With this service, companies and laboratories ensure that they report reliable and reproducible results in all their studies, guaranteeing the traceability and viability of analyzes obtained.

References

  1. J Bote, et al. Novel ex-vivo database of a murine model of colorectal cancer using optical coherence tomography. Surg Endosc (2022) 36:S325–S674
  2. V Lucas-Cava, et al. Prostatic artery occlusion: Initial findings on pathophysiological response in a canine prostate model. Translational Andrology and Urology. Transl Androl Urol 2022.
  3. Soria F, et al. Heparin coating in biodegradable ureteral stents does not decrease bacterial colonization-assessment in ureteral stricture endourological treatment in animal model. Transl Androl Urol. 2021 Apr;10(4):1700-1710.
  4. Baez-Díaz C, et al. Microencapsulated Insulin-Like Growth Factor-1 therapy improves cardiac function and reduces fibrosis in a porcine acute myocardial infarction model. Sci Rep. 2020 Apr 28;10(1):7166.
  5. Moreno-Lobato, B, et al. Use of nanomedicine in preclinical wound healing studies. FarmaJournal; Salamanca T 4, N.º 1, (Feb 2019): 178.
  6. Picado Román, N, et al. Drugs most used in experimental animals in a Research Center. FarmaJournal; Salamanca T 4, N.º 1, (Feb 2019): 236.
  7. Moreno-Lobato B, et al. Analytical Validation Study of Hematological Parameters under Good Laboratory Practice Regulations in Different Laboratory Animal Species. Thromb Haemost Res. 2019; 3(1): 1018.
  8. Ballestín A, et al. A Pre-clinical Rat Model for the Study of Ischemia reperfusion Injury in Reconstructive Microsurgery. J Vis Exp. 2019 Nov 8;(153).
  9. R Blázquez , et al. Altered hematological, biochemical and immunological parameters in a porcine myocardial infarction model: predictive biomarkers for the severity of myocardial infarction. Veterinary Immunology and Immunopathology 205 (2018) 49–57.
  10. Vela FJ, et al. Evaluation of antigen-induced synovitis in a porcine model: Immunological, arthroscopic and kinetic studies. BMC Vet Res. 2017. Apr 7;13(1):93.
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U14-S06. Lymphocyte proliferation assay

Lymphocyte proliferation assay

The lymphocyte proliferation assay service offers a robust platform for evaluating the proliferative responses of lymphocytes in vitro. Utilizing state-of-the-art stimulation protocols and specialized proliferation assays, this service enables precise quantification of lymphocyte activation and proliferation rates. By mimicking physiological conditions in a controlled laboratory setting, researchers can investigate the dynamic behavior of lymphocytes in response to various stimuli. This service plays a pivotal role in elucidating immune responses, assessing immune competence, and evaluating the efficacy of immunomodulatory interventions, ultimately contributing to advancements in both basic immunology and clinical therapeutics.

Customer benefits

U14 Cell Therapy Unit offers researchers precision, efficiency, and expertise, streamlining experiment. With stringent quality control, ISO 9001-2015 and GLP compliant, tailored solutions, and access to advanced equipment, researchers can focus on innovation and core objectives, driving scientific progress forward.

Target customer

This service is essential for organizations involved in Research and Development, including pharmaceutical companies, academic institutions for biomedical research, biotechnology firms for bioprocessing, and regulatory agencies for in vitro studies.

Additional information

Selected references:

  • de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther.  2023.  Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551.  https://doi.org/10.1186/s10020-023-00646-1
  • Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM.  2022.  Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells   Animals.  MDPI.  12-20.  ISSN 2076-2615.  https://doi.org/10.3390/ani12202798
  • Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther.  2021.  A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
  • de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10).  2021.  IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.  MDPI.  22-22.  ISSN 1422-0067.  WOS (0) https://doi.org/10.3390/ijms222212177
  • López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269.  https://doi.org/10.1007/s12015-019-09926-y

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U14-S05. Determination of cytokine concentration by flow cytometry

Determination of cytokine concentration by flow cytometry

The determination of cytokine concentration by flow cytometry service represents a cutting-edge approach to cytokine analysis within the realm of biomedical research and pharmaceutical investigation. Through the utilization of advanced flow cytometry techniques and specialized staining protocols, this service enables the precise quantification of intracellular cytokines across distinct cell subsets distinguished by surface markers. This service facilitates the elucidation of cytokine profiles within specific cell populations, shedding light on their roles in health, disease, and therapeutic interventions.

Customer benefits

U14 Cell Therapy Unit offers researchers precision, efficiency, and expertise, streamlining experiment. With stringent quality control, ISO 9001-2015 and GLP compliant, tailored solutions, and access to advanced equipment, researchers can focus on innovation and core objectives, driving scientific progress forward. By delving into the intracellular milieu, researchers gain invaluable insights into the intricate regulatory networks governing immune responses and cellular signaling pathways.

Target customer

This service is essential for organizations involved in Research and Development, including pharmaceutical companies, academic institutions for biomedical research, biotechnology firms for bioprocessing, and regulatory agencies for in vitro studies.

Additional information

Selected references:

  • de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther.  2023.  Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551.  https://doi.org/10.1186/s10020-023-00646-1
  • Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM.  2022.  Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells   Animals.  MDPI.  12-20.  ISSN 2076-2615.  https://doi.org/10.3390/ani12202798
  • Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther.  2021.  A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
  • de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10).  2021.  IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.  MDPI.  22-22.  ISSN 1422-0067.  WOS (0) https://doi.org/10.3390/ijms222212177
  • López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269.  https://doi.org/10.1007/s12015-019-09926-y
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U14-S04. Cytokine quantification assays

Cytokine quantification assays

The cytokine quantification assays service is at the forefront of cytokine analysis in biomedical research and pharmaceutical development. Leveraging advanced immunoassay techniques and specialized expertise, this service offers precise quantification of cytokines present in cell culture supernatants or animal fluids. Through meticulous analysis, researchers gain valuable insights into the intricate signaling networks and immune responses underlying various physiological and pathological processes. From characterizing inflammatory cascades to evaluating therapeutic interventions, our service provides a robust platform for investigating cytokine dynamics and their implications for health and disease.

Customer benefits

U14 Cell Therapy Unit offers researchers precision, efficiency, and expertise, streamlining experiment. With stringent quality control, ISO 9001-2015 and GLP compliant, tailored solutions, and access to advanced equipment, researchers can focus on innovation and core objectives, driving scientific progress forward.

Target customer

This service is essential for organizations involved in Research and Development, including pharmaceutical companies, academic institutions for biomedical research, biotechnology firms for bioprocessing, and regulatory agencies for in vitro studies.

Additional information

Selected references:

  • de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther.  2023.  Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551.  https://doi.org/10.1186/s10020-023-00646-1
  • Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM.  2022.  Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells   Animals.  MDPI.  12-20.  ISSN 2076-2615.  https://doi.org/10.3390/ani12202798
  • Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther.  2021.  A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
  • de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10).  2021.  IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.  MDPI.  22-22.  ISSN 1422-0067.  WOS (0) https://doi.org/10.3390/ijms222212177
  • López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269.  https://doi.org/10.1007/s12015-019-09926-y
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U11-S2. Formulation Lab

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Nanomedicine Summer School

The second Summer School in Nanomedicine coordinated by Simó Schwartz, Scientific Director of NANBIOSIS unit 20,  was held at Vall d’Hebron hospital on 28 and 29 September. Dr. Schwartz has been the president of the European Society for Nanomedicine (ESNAM), a European scientific society focused on the clinical application of nanomedicine. From this society tasks are carried out to promote the research and the use of nanomedicine in the health sector, as well as to facilitate the exchange of knowledge in this area at European level among all the actors involved, Academy, industry and entities regulators.

CIBER-BBN, partner of NANBIOSIS, as sponsor of ESNAM, has collaborated in the organization of this event. Lectures were given by some Scientific Directors  or Coordinators  of NANBIOSIS units as Jaume Veciana, Ibane Abasolo, Laura Lechuga and Antoni Villaverde .

Photos of the meeting can be viewed in this link.

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U26-S04. In vivo NMR of small animals (zebrafish,Drosophila)

In vivo NMR of small animals (zebrafish, Drosophila)

The service is intended for obtaining images of small animals such as zebrafish, Drosophila. Also longitudinal studies can be performed. Thanks to a 14 T NMR equipment equipped with a micro-image probe.

Customer benefits

The service is integrated in the University of Valencia core facility that ensures the correct maintenance and the offsite service runed by technicians under ISO 9001.

Target customer

The primary audience are pre-clinic research groups that require punctual or longitudinal studies on such small animals with high resolutions.

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U20-S11. Histological porcessing, section and staining (Remote) OUTSTANDING

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