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Nanbiosis

U7-E02. ToF-SIMS (Time of the Flight Secondary Ion Mass Spectroscopy)

• ToF-SIMS (Time of the Flight Secondary Ion Mass Spectroscopy)

ION-TOF IV (IONTOF, Münster, Germany) equipped with the state-of-the –art Bi cluster primary ion gun for analysis, and O2 and Cs ion guns for sputtering in the depth profiling mode.
Charge compensation capability using a pulsed electron-gun and/or O2 flood gas allows surface analysis and depth profiling of highly insulating samples
Large sample stage capable of holding wafers up to 3″ in diameter/width and 0.5″ in thickness. Samples sizes from few mm up to 10cm. Motorized sample stage that allows for automated analysis of large areas. Heating/cooling stage.

• High mass resolution, to distinguish species of similar nominal mass (mass resolution is at least 0.00x amu); Particles with the same nominal mass (e.g. Si2 and Fe, both with amu = 56 ) can be clearly distinguish.
• Mass range of 0-10,000 amu; ions (positive or negative), isotopes, and molecular compounds (including polymers, organic compounds, and up to amino acids fragments) can be detected.
• High sensitivity for trace elements or compounds, on the order of ppm to ppb for most species;
• Elemental and chemical mapping on a sub-micron scale;
• Depth profiling capabilities; sequential sputtering of surfaces allow analysis of elemental composition on materials from 1nm up to 10µm in depth (typical sputtering rates are ~10nm/minute).
• Retrospective analysis. Every pixel of a ToF-SIMS map represents a full mass spectrum. This allows an analyst to retrospectively produce maps for any mass of interest, and to interrogate regions of interest (ROI) for their chemical composition via computer processing after the dataset has been instrumentally acquired.
• Surveys of all masses on material surfaces; these may include single ions (positive or negative), individual isotopes, and molecular compounds;
• Surface analysis of insulating and conducting samples;

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U7-E01. Ebeam/SEM (Nova NanoSEM 230/Raith)

• Nova NanoSEM 230

›› High and Low Vacuum field emission Scanning
Electron Microscope (SEM ) incorporating:

•• Resolution: 1.6 nm at 1 kV (ETD and TLD detectors) in High Vacuum.
•• Resolution: 1.8 nm at 3 kV (LVD detectors) in Low Vacuum.
•• Field emission filament -> High-resolution images even of non-conductive samples.
•• Backscattered electron detector (vCD detector):
High-resolution images of backscattered electrons.
•• 6-channel preamplifier for detectors of solid state.
•• Compressor.
•• Cryo Can, anti-contamination system.
•• Thermoflex closed wáter circuit.
›› Electron beam lithography system with:
•• High writing resolution (maximum speed of 6 MHz and control of the dwell time < 2 ns). •• Electrostatic Beam Blanker. •• Picoammeter exchanger box. •• Keithley picoammeter. •• Advanced nanolithography system for microscope. •• Beam Blanker amplification electronics •• Software for correcting the Proximity Effect. •• Universal sample carrier for Lithography. •• EBL Starter kit.

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U14-S02. Flow Cytometry

Flow Cytometry

The Flow Cytometry service occupies a leading position in biomedical and pharmaceutical research, as well as in cellular and molecular biology. Utilizing cutting-edge instrumentation and specialized expertise, this service offers a diverse array of analytical techniques crucial for elucidating the nuanced intricacies of cellular behavior. Ranging from precise cellular phenotyping to dynamic proliferation assays, and encompassing assessments of viability alongside in-depth investigations into apoptosis, our service provides a comprehensive toolkit for the thorough interrogation of cellular characteristics and responses.

Customer benefits

U14 Cell Therapy Unit offers researchers precision, efficiency, and expertise, streamlining experiment. With stringent quality control, ISO 9001-2015 and GLP compliant, tailored solutions, and access to advanced equipment, researchers can focus on innovation and core objectives, driving scientific progress forward.

Target customer

This service is essential for organizations involved in Research and Development, including pharmaceutical companies, academic institutions for biomedical research, biotechnology firms for bioprocessing, and regulatory agencies for in vitro studies.
Through the precise analysis enabled by flow cytometry, researchers gain invaluable insights into cellular behavior, facilitating the advancement of preclinical trials and therapeutic development.

Additional information

Selected references:

  • de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther.  2023.  Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551.  https://doi.org/10.1186/s10020-023-00646-1
  • Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM.  2022.  Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells   Animals.  MDPI.  12-20.  ISSN 2076-2615.  https://doi.org/10.3390/ani12202798
  • Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther.  2021.  A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
  • de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10).  2021.  IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.  MDPI.  22-22.  ISSN 1422-0067.  WOS (0) https://doi.org/10.3390/ijms222212177
  • López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269.  https://doi.org/10.1007/s12015-019-09926-y
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U14-S01. Cell cultures

Cell cultures

The cell culture service plays a pivotal role in the realm of biomedical and pharmaceutical research, as well as in the broader fields of cellular and molecular biology. It provides specialized support for the development, production, and characterization of cell therapies tailored for preclinical trials. With a focus on mesenchymal stem cells (MSCs), the service encompasses the entire spectrum of cell culture activities, including isolation, expansion, and thorough characterization of these crucial cellular components. Additionally, the service extends its offerings to include essential assays such as proliferation, viability, and cytotoxicity assessments, all of which are indispensable in the rigorous evaluation of potential therapies during preclinical trials.

Customer benefits

U14 Cell Therapy Unit offers researchers precision, efficiency, and expertise, streamlining experiments. With stringent quality control, ISO 9001-2015 and GLP compliant, tailored solutions, and access to advanced equipment, researchers can focus on innovation and core objectives, driving scientific progress forward.

Target customer

This service is essential for organizations involved in Research and Development, including pharmaceutical companies, academic institutions for biomedical research, biotechnology firms for bioprocessing, and regulatory agencies for in vitro studies. Cell Culture service empowers researchers and stakeholders with the tools and insights necessary to advance the frontiers of regenerative medicine and therapeutic innovation.

Additional information

Selected references:

  • de Pedro, Maria Ageles; Pulido, Maria; Álvarez, Veronica; Marinaro, Federica; Marchena, Ana Maria; Sanchez-Margallo, Francisco Miguel; Casado, Javier G.; Lopez, Esther.  2023.  Menstrual blood-derived stromal cells: insights into their secretome in acute hypoxia conditions. MOLECULAR MEDICINE. SPRINGER. 29-1. ISSN 1076-1551.  https://doi.org/10.1186/s10020-023-00646-1
  • Cristobal JI; Duque, FJ; Uson-Casaus J; Barrera R; López E; Perez-Merino EM.  2022.  Complete Blood Count-Derived Inflammatory Markers Changes in Dogs with Chronic Inflammatory Enteropathy Treated with Adipose-Derived Mesenchymal Stem Cells   Animals.  MDPI.  12-20.  ISSN 2076-2615.  https://doi.org/10.3390/ani12202798
  • Marinaro, Federica; Silva, Joana M; Barros, Alexandre A; et al; Lopez, Esther.  2021.  A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences. MDPI. 22-24. ISSN 1422-0067. WOS (0) https://doi.org/10.3390/ijms222413385
  • de Pedro, Maria Angeles; Gomez-Serrano, Maria; Marinaro, Federica; et al; Lopez, Esther (AC); Casado, Javier G.(4/10).  2021.  IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.  MDPI.  22-22.  ISSN 1422-0067.  WOS (0) https://doi.org/10.3390/ijms222212177
  • López E; Blazquez R; Marinaro F; et al; Casado JG. 2019. The Intrapericardial Delivery of Extracellular Vesicles from Cardiosphere-Derived Cells Stimulates M2 Polarization during the Acute Phase of Porcine Myocardial Infarction. STEM CELL REVIEWS AND REPORTS. SPRINGER. 16-3, pp.626-626. ISSN 2629-3269.  https://doi.org/10.1007/s12015-019-09926-y
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U14-E01. Cell culture rooms

Cell Culture Room 1:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal).
CO2 incubator Heracell 150 (Thermo)
Nomarski phase inverted microscope and fluorescencia con cámara (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)

Cell Culture Room 2:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal)
CO2 incubator Heracell 150 (Thermo)
CO2 incubator Heracell 150 with CO2 control (Thermo)
phases inverted microscope with fluorescence (NIKON)
Vacuum Pump ME4 (Vacuubrand)
Cell Counter (Invitrogen)

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U14-E02. Molecular biology

Molecular Biology
Fume Hood (Formimetal)
Stereo microscope with camera and digital image set (NIKON)
light source (for the glass) (NIKON)
Refrigerated centrifuge for microtubs Biofuge® Pico (HERAEUS)
Digital circulation thermostatic bath (GRANT)
Thermostatic bath (for cell cultures temperar medios) (MEDIGEN))
Pipette controller pipette Powerpette-plus (IZASA)
Pipettes de volumen variable continuo with accessories (RAININ)
Vertical Freezer -20ºC (FIOCHETTI)
Freezer +2/+8ºC (CLASS ELECTRONIC)
Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80ºC) (HERAEUS)
Crioconservtion system with accessories
Liquid Nitrogen tank (taylor wharton)
Thermocycler (THERMO)
Sterilization incubator (Modelo UT ) (HERAEUS)
G-Box Translumitator and accessories (PC, Sofware…) (SYNGENE)
Electrophoresis system (IZASA)
Electrophoresis vertical jar
Electrophoresis horizontal (big) jar
Electrophoresis horizontal (small) jar
Rotary shaker with incubation (HEIDOLPH)
Magnetic stirrer with heating (ARE)
Analytical balance 210gr, 0,01 gr. (ACCULAB)
Precision analytical balance (OHAUS)
Ultrasonic water bath (SELECTA)
Crushed ice machine (Schottman)
Microwave (LG)
Pipette controller (POWERPETTE PLUS)
Thermoblock (Labolan)
Vortex 230 V, EU (VWR)
Freezer +4ºC/-20ºC
UV mini-1240 Spectrophotometer (SHIMADZU)
ELISA plate reader, Synergy MX (BIOTEK)
Flow cytometry FACSCalibur (BD)
pH-Meter (Symphony)
i-Blot (Invitrogen)
Autoclave

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U13-E02. Machines for mechanical tests

Machines for mechanical tests:

• Instron MicroTester 5848 and 5548: electromechanical machines for displacement or load control tests:

•• load range from 1mN to 2kN at full scale.
•• load cells of:+/- 5, 50, 500 and 1000N.
•• synchronous data capture in all the channels up to
500 Hz.
• Biaxial Instron MicroTester 8874: axial-torsional, servohydraulic testing machine:
•• load capacity of 25 kN and 100 Nm.
•• fatigue tests at a low number of cycles.

›› Software for data control and analysis.
›› Accessories and tools especially designed by the group, which allow a wide flexibility in designing tests, allowing the verification and/or validation of theoretical models.
›› AVE video extensometer connected to the machines; resolution of 0.5 μm. To determine the axial and transverse deformation without contact (tension, dynamic properties and viscoelasticity) of the samples.

• E1000 Test Instrument: Dynamic systems to offer slow-speed static testing and high-frequency dynamic fatigue testing with hundreds of Hertz capability: Load range: 250 N to 2.0 KN.
• Nano BIONIX MTS: For nanomechanical characterization of biomaterials. Load range to 0.001N to 0.5N.

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U13-E01. MicroCT GE eXplore Locus SP (GE)

MicroCT GE eXplore Locus SP (GE): Computed X-ray Axial Tomograph for the non-destructive study of the microstructure and in vitro tomography: ›› Samples of up to 40 mm in diameter; resolution of 10 μm. ›› Different types of tissues, implants, scaffolds, etc. ›› Complete “section-by-section” imaging with a single rotation (cone beam volumetric technology); excellent image quality and “signal-to-noise” ratio. ›› “Bone Analysis Tool” software provided with: •• BMD (Bone Mineral Density): It calculates the density of minerals in the bone, the bone volume fraction and the mineral content in the bone. •• Anisotropy: It determines the degree of symmetry and the orientation of the trabecular structure. •• Cortical Analysis: It determines “section-by-section” the width, surface area and the BMD value of the cortical bone. •• Stereology: It provides the bone volume fraction, the ratio between the surface of the bone and its volume and the width of the trabecular plate. •• Direct Measures: It determines the local trabecular width of a bone and allows obtaining a visual representation of this local width.

Temporarily OUT OF ORDER

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U13-S01. MicroCT

MicroCT

X-Ray image for biomaterials, scaffolds and biological tissues.

Customer benefits

An axis inside is able to apply forces.
Non-destructive view of internal structure pre and post force.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

Additional information

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U13-S03. Fatigue tests Instron 8874

Fatigue tests Instron 8874

Fatigue tests allow us to know how is the behaviour when cyclic load are applied. It is used for life expectancy of biomaterials and biological tissues.

Customer benefits

High full-scale cell load.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

Additional information

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