2015 - Nanbiosis

U24-E05. 1.5T Magnetic Resonance Imaging (MRI) system

Magnetic Resonance Imaging (MRI), Philips- INTERA 1.5T. The MRI equipment (Philips Intera 1.5T) enables the acquisition –using non-invasive techniques- of high definition images of any body organ at different planes, focusing on large animals as experimental models.

The availability of high precision images during research activities enables the researcher to choose correct clinical treatment. This equipment is also used in the follow up of some R&D + innovation lines; during the post operative period of experimental subjects, i.e. in activities such as cellular therapy to measure the therapeutic effect or to evaluate implanted biomaterials.

Thus, this MRI system provides a reliable diagnosis and follow up of certain diseases, allowing the evaluation of state-of-the-art health technologies and their benefits and helping to establish their clinical indications.

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U23-S03. Micromanipulation and microinjection

Micromanipulation and microinjection

Within the Assisted Reproduction Unit we have a laboratory equipped with two micromanipulation and microinjection equipment that allows us to inject the sperm into the cytoplasmic cavity of the oocyte (ICSI), in order to carry out fertilization. The advantage that this equipment offers is that it allows us to carry out in vitro fertilization without having to have a large number of sperm and oocytes. Furthermore, to be able to carry out this technique with oocytes from different animal species, we have a piezoelectric device to perform this intracytoplasmic injection without damaging the oocyte of the specific animal species.

Customer benefits

Having these micromanipulation and microinjection equipment allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out this technique in the human clinic. In addition, the implementation of this ICSI technique and the handling and use of micromanipulators and microinjectors allows for practical training for master’s students and specialized courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out with animal species, so our laboratory is suitable for it. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process without damaging the oocyte.

Additional information

Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
Optimizations of an ovum pick up protocol in Donkey.
Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
Reproduction. Fertility and Development Sep;34(15):980-990
https://doi.org/10.1071/RD22041
4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
https://doi.org/10.1093/molehr/gaaa075
Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
Methods X. vol 7, pp:1-9; 2020, 100767
https://doi.org/10.1016/j.mex.2019.12.008

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U23-S02. Embryo culture media viability studies

Embryo culture media viability studies

Through this service, and through the time-lapse incubator that we have, embryonic development studies can be carried out by enriching the culture media with different compounds or additives to later verify which of them produces better embryonic development and better quality of the embryos, through viability and embryokinetic studies.

Customer benefits

In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out quality control for Human Reproduction clinics, studies of in vitro fertilization and viability of embryo culture media, of mouse embryos or other animal species, whether that we have piezo electric. Furthermore, as we have a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
Optimizations of an ovum pick up protocol in Donkey.
Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
Reproduction. Fertility and Development Sep;34(15):980-990
https://doi.org/10.1071/RD22041
4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
https://doi.org/10.1093/molehr/gaaa075
Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
Methods X. vol 7, pp:1-9; 2020, 100767
https://doi.org/10.1016/j.mex.2019.12.008

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U23-S01. In vitro fertilisation studies and embryo culture media viability

In vitro fertilisation studies and embryo culture media viability

Through this service, in vitro fertilization processes can be carried out, including IVF and ICSI, of oocytes and sperm from different animal species, including mice, which are the animal species we mainly work with. To carry out this fertilization we have cabins, heated laminar flow, with stereomicroscopes included, plates, incubators with different gas systems and in order to evaluate the development of the embryos in real time we have recently incorporated a time lapse incubator, which allows us to carry out embryokinetic studies without having to remove the plates and the embryo from the incubator which improves the quality and viability of the embryo.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

Additional information

Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
Optimizations of an ovum pick up protocol in Donkey.
Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
Reproduction. Fertility and Development Sep;34(15):980-990
https://doi.org/10.1071/RD22041
4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
https://doi.org/10.1093/molehr/gaaa075
Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
Methods X. vol 7, pp:1-9; 2020, 100767
https://doi.org/10.1016/j.mex.2019.12.008

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U21-S01. In vivo Efficacy Assays of drugs, nanomedicines, biomaterials and others

In vivo Efficacy Assays of drugs, nanomedicines, biomaterials and others

Preclinical studies for the evaluation of medical treatments, medical devices and surgical techniques in a controlled environment prior to their application in patients.
Conducting studies -both in human and veterinary medicine- of in vitro toxicity, in vivo toxicity, tolerance, pharmacodynamics, test product administration and procurement of non-clinical specimens, biocompatibility of medical devices, murine model embryo transfers, intracytoplasmic microinjection, oocyte vitrification, measurement and analysis of Natural Killer (NK) cell samples, seminogram and semen studies.
Creation of experimental animal models of various human diseases such as diabetes, obesity, hypercholesterolemia, hypertriglyceridemia, acute and chronic myocardial infarction, prostatic hyperplasia, ulcers, pleural adhesions, constrictive pericarditis and abdominal aortic aneurysm, among others. Possibility of working with a variety of animal species: rodents, rabbits, dogs, cats, pigs and sheep.
Application of experimental animal models for research in several longitudinal areas such as cardiovascular, hepatic and digestive, respiratory and systemic diseases, endocrinology and urology, gynecological and reproductive diseases, prostheses, among others.

Customer benefits

Preclinical studies can be carried out under good laboratory practice (GLP 23.05/001 AEMPS), optimal for reporting to the AEMPS, EMA and FDA.
In vivo toxicity, tolerance, pharmacodynamics, test product administration and non-clinical specimen collection and biocompatibility studies of medical devices are carried out according to OECD decision C(89)87.
Registered facilities for the use and breeding of experimental animals, which has an Animal Welfare Ethics Committee and a pharmacy and formulation laboratory.

Target customer

Entities involved in the research, development or commercialization of medical devices or treatments such as pharmaceutical and biotechnology companies, academic and scientific researchers, medical device companies, research institutions and laboratories, and governmental and regulatory organizations.

Additional information

Jiménez-Holguín J, Lozano D, Saiz-Pardo M, de Pablo D, Ortega L, Enciso S, Fernández-Tomé B, Díaz-Güemes I, Sánchez-Margallo FM, Portolés MT, Arcos D. Osteogenic-angiogenic coupled response of cobalt-containing mesoporous bioactive glasses in vivo. Acta Biomater. 2024 Mar 1;176:445-457. doi: 10.1016/j.actbio.2024.01.003. Epub 2024 Jan 6. PMID: 38190928.
Lucas-Cava V, Sánchez-Margallo FM, Moreno-Lobato B, Dávila-Gómez L, Lima-Rodríguez JR, García-Martínez V, López-Sánchez C, Sun F. Prostatic artery occlusion: initial findings on pathophysiological response in a canine prostate model. Transl Androl Urol. 2022 Dec;11(12):1655-1666. doi: 10.21037/tau-22-423. PMID: 36632152; PMCID: PMC9827397.
Arcos D, Gómez-Cerezo N, Saiz-Pardo M, de Pablo D, Ortega L, Enciso S, Fernández-Tomé B, Díaz-Güemes I, Sánchez-Margallo FM, Casarrubios L, Feito MJ, Portolés MT, Vallet-Regí M. Injectable mesoporous bioactive nanoparticles regenerate bone tissue under osteoporosis conditions. Acta Biomater. 2022 Oct 1;151:501-511. doi: 10.1016/j.actbio.2022.07.067. Epub 2022 Aug 3. PMID: 35933104.
Soria F, de La Cruz JE, Caballero-Romeu JP, Pamplona M, Pérez-Fentes D, Resel-Folskerma L, Sanchez-Margallo FM. Comparative assessment of biodegradable-antireflux heparine coated ureteral stent: animal model study. BMC Urol. 2021 Feb 28;21(1):32. doi: 10.1186/s12894-021-00802-x. PMID: 33639905; PMCID: PMC7916282.
Marinaro F, Casado JG, Blázquez R, Brun MV, Marcos R, Santos M, Duque FJ, López E, Álvarez V, Usón A, Sánchez-Margallo FM. Laparoscopy for the Treatment of Congenital Hernia: Use of Surgical Meshes and Mesenchymal Stem Cells in a Clinically Relevant Animal Model. Front Pharmacol. 2020 Sep 25;11:01332. doi: 10.3389/fphar.2020.01332. PMID: 33101010; PMCID: PMC7546355.

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U21-E01. Experimental operating rooms for small and large animal

Experimental operating rooms for small and large animal (inhalation anesthesia workstation, infusion pumps, electrocoagulation systems, laparoscopic surgery systems, endoscopic surgery, microsurgery, interventional and endoluminal surgery, orthopedics, etc.)

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U21-E02. Rooms for preparation and preanesthesia

Rooms for preparation and preanesthesia

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U21-E03. Sterelization rooms for medical instruments and tools

Sterelization rooms for medical instruments and tools

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U21-E04. Integrated operating room

(Karl Storz OR1, Karl Storz Spies) with possibility of integrating endoscopy images, cameras, documents, communications, etc., Allowing data transfer, videoconferences from a touch screen or voice.
Single-pole and two-pole electrosurgical generators (Force Triad Valleylab, Karl Storz AUTOCON® II 200, Erbe ICC 300, Erbe VIO 310, Erbe ICC 200, Ultracision Harmonic scalpel, Ligasure Valleylab, Sonicision)

Laparoscopic surgery equipments: nineteen high definition laparoscopic towers (Karl Storz-Endoskope) equipped with monitors, cold light source, insuflator (Thermoflator), susction and irrigation system (Endomat) and optics with different calibers and view angles. In addition, four of them have incorporated a Visualization Enhancement Tools system (Karl Storz Image 1 SpiesTM) for fluorescence-based procedures in order to visualize what is hidden from view under conventional white lightand and another two possessed. The KARL STORZ 3D system which offers the surgeon excellent depth of field combined with outstanding hand-eye coordination.

Endoscopic equipment: Digestive video-endoscope system (Serie 200 Fujinon®) Double Balloon Enteroscopy system (Fujinon® Series 200), Video colonoscopic systems (Olympus® ViseraOlympus® Visera), Video bronchoscopic system (Serie 200 Fujinon®Fujinon® Series 200), Semi-rigid ureteroscopy system (Wolf® 9 Fr.Wolf® 9 Fr), Flexible ureteropieloscopy system (Wolf® 9 Fr.Wolf® 9 Fr). Semi-rigid ureteroscopy system (Storz® 9.5 Fr., 43 cm long). Semi-rigid ureteroscopy system (Storz® 7 Fr, 43 cm long)

Surgical microscopes Zeiss Opmi Pico (13 units), Zeiss Opmi Movena and Zeiss Opmi Pentero 800 (with fluorescence module).

Ophthalmology equipment: corneal topographer (CSO Eye Top 2005 V.1.0), slit lamp (SL-D4 TopCon) and phacoemulsification system (Sovereign White Star AMO-ABBOTT 2 units and Sovereign Compact White Star AMO-ABBOTT 1 unit), Tonometer (TonoVet ICare), Non-contact lens system vitrectomy SDI 4b (5 units), Optical coherence tomography (OCT) (SLO Optos)

Odontology equipment: Ultrasonic generator for treatment of dental plaque (Newtron P5, Acteon),

Vascular Surgery: Laser Doppler Imager and Transit Time Ultrasound Flowmeter

Anesthesia equipment: 13 high performance anesthesia Workstation (Maquet Flow-i, Dameca Siesta i Whispa, Dameca MRi 508, Heinen+Lowenstein Mod. Leon, Heinen+Lowenstein Mod. Leon Plus, Ohmeda Excel 210 SE, Ohmeda Excel 110 SE, Temel Supra CM, Datex Ohmeda S/5TM); Anesthesia machine for rodents (Surgivet); Transport ventilator (Transpac 200D);

13 isoflurane, sevoflurane and desflurane vaporizer (Maquet, Dameca Sigma Delta, Dräger, Abbott laboratories, Datex Ohmeda Tec 6); 14 cardiovascular monitors (Hewlett-Packard, Mindray PM-700, Mindray MEC 1000, Mindray BeneView T5, General Electric Dash 3000, General Electric Dash 4000, Drager Infinity Gamma, Datex Ohmeda S/5TM, Philips MX-450, Philips Expression MR200); 11 capnography systems (Maquet, Drager Vamos, General Electric RAC 2A, Ohmeda Biox 3740, Sentec, Ohmeda Capnomac Ultima, Dameca siesta) and pulse oximeter (Ohmeda Biox 3740); Cardiac output monitoring system (PICCO Plus) and two bispectral electroencephalogram monitor (BIS-A 1050, BIS –XP A 2000), parasympathetic tone activity monitor (MDoloris PTA) and muscle relaxation monitor (Organon TOF Watch); Four infusion pumps (Argus 600S, Braun Perfusor Compact, Imed Gemini PC1, Becton Dickinson); Two desfibrillators (Zoll Mseries, Nihon Kohden Cardiolife)

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Nanbiosis

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U24-E05. 1.5T Magnetic Resonance Imaging (MRI) system

U23-S03. Micromanipulation and microinjection

Micromanipulation and microinjection

U23-S02. Embryo culture media viability studies

Embryo culture media viability studies

U23-S01. In vitro fertilisation studies and embryo culture media viability

In vitro fertilisation studies and embryo culture media viability

U21-S01. In vivo Efficacy Assays of drugs, nanomedicines, biomaterials and others

In vivo Efficacy Assays of drugs, nanomedicines, biomaterials and others

U21-E01. Experimental operating rooms for small and large animal

U21-E02. Rooms for preparation and preanesthesia

U21-E03. Sterelization rooms for medical instruments and tools

U21-E04. Integrated operating room

U20-E03. Animal Facility