U6-E14. Microcalorimeter for measurements under Isothermal Titration Calorimetry (ITC)
MicroCal VP-ITC – Malvern
Description:
Isothermal titration calorimetry (ITC) measures the binding affinity and thermodynamics between two biomolecules. When substances bond, heat is released or absorbed. Measuring this heat change in an ITC experiment allows precise determination of the binding constants (KD), stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), providing a complete thermodynamic profile of the molecular interaction in a single experiment.
The MicroCal VP-ITC system is a powerful, sensitive and easy-to-use calorimeter that offers the possibility of investigating any biomolecular interaction. This rapid, convenient, label-free, and solution-based technique provides the information needed to characterize the molecular interactions of proteins, antibodies, nucleic acids, and other biomolecules.
Technical specifications:
• Cell type: Non-reactive Hastelloy
• Sample volume 2 mL
• Volume per cell: 1.4 mL
• Volume injected: 0.3 mL
• Mixing speed: user selectable
• Response time: user selectable
• Working temperature: 2º to 80º C
Applications:
• Characterization of molecular interactions of proteins, antibodies, nucleic acids and other biomolecules.
• Kinetic enzyme.
• Evaluation of the effect of molecular structural changes on binding mechanisms.
• Evaluation of biological activity.