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Posts on Jan 1970

U24-E01. Fluoroscopy

  • Cardiac Electrophysiology Laboratory: radiofrecuency ablation system and multicanal poligraph.
  • Fluoroscopy: BV PULSERA Philips Medical Systems
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U24-E02. Digital subtraction angiography system

  • Digital Subtraction Angiography (DSA) (PHILIPS BV- Pulsera Philips Medical System, Netherlands).
  • Digital Subtraction Angiography with flat panel detector (DSA) (PHILIPS Veradius Unity
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U24-E03. Ultrasound scanner

  • Ultrasound scanner (Panther Ultrasound Scanner Type 2002, B&K MEDICAL, Inc. DENMARK.
  • Ultrasound scanner (HDI5000. Philips Medical Systems, Netherlands).
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U24-E0x. Intravascular ultrasound

Intravascular ultrasound (IVUS) (ClearView Ultra. Boston Scientific)

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U24-E0x. Carbon dioxide (CO2) injector for angiographies

Carbon dioxide injector for angiographies (CO2JECT Injector, ANGIODYNAMICS, Inc. NY, USA).

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U24-E04. Computed Tomography (CT) scanner

Computed Tomography (CT), Philips BRILLIANCE CT-6. The CT system includes 24 solid-state detectors that enable the acquisition of 6 simultaneous slices per 360º rotation, with a minimal slice thickness (0.6 mm) at an imaging time of 0.75 seconds per 360º for a full body scan.

These features make a high working speed possible with excellent image quality. The images can be processed in either basic or advanced mode from a workstation with the following software: Angio-CT, CINE mode, dynamic focal point reconstruction, multiplanar reconstruction (linear, curvilinear, etc), 3D multi tissue reconstruction (MIP, VR…), small volumes 3D analysis, and measurement tools for quantification (Q-CTA).

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U24-E05. 1.5T Magnetic Resonance Imaging (MRI) system

Magnetic Resonance Imaging (MRI), Philips- INTERA 1.5T. The MRI equipment (Philips Intera 1.5T) enables the acquisition –using non-invasive techniques- of high definition images of any body organ at different planes, focusing on large animals as experimental models.

The availability of high precision images during research activities enables the researcher to choose correct clinical treatment. This equipment is also used in the follow up of some R&D + innovation lines; during the post operative period of experimental subjects, i.e. in activities such as cellular therapy to measure the therapeutic effect or to evaluate implanted biomaterials.

Thus, this MRI system provides a reliable diagnosis and follow up of certain diseases, allowing the evaluation of state-of-the-art health technologies and their benefits and helping to establish their clinical indications.

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U23-S03. Micromanipulation and microinjection

Micromanipulation and microinjection

Within the Assisted Reproduction Unit we have a laboratory equipped with two micromanipulation and microinjection equipment that allows us to inject the sperm into the cytoplasmic cavity of the oocyte (ICSI), in order to carry out fertilization. The advantage that this equipment offers is that it allows us to carry out in vitro fertilization without having to have a large number of sperm and oocytes. Furthermore, to be able to carry out this technique with oocytes from different animal species, we have a piezoelectric device to perform this intracytoplasmic injection without damaging the oocyte of the specific animal species.

Customer benefits

Having these micromanipulation and microinjection equipment allows us to offer this service to embryologists who wish to train in this technique and acquire the necessary skills to be able to carry out this technique in the human clinic. In addition, the implementation of this ICSI technique and the handling and use of micromanipulators and microinjectors allows for practical training for master’s students and specialized courses in human or animal assisted reproduction.
Because our laboratory is one of the few that researches assisted reproduction with animal models and has all this equipment, human reproduction clinics that want to perform tests with animal oocytes or embryos can perform their tests.
In our laboratory, since experiments can only be carried out with animal species, so our laboratory is suitable for it. In addition, the studies can also be carried out under regulatory requirements, since the institution that performs them is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This service can provide assistance to all those Human Reproduction companies and clinics that want to carry out in vitro fertilization studies using Micromanipulation and microinjection techniques of mouse embryos or other animal species, since we have a piezoelectric system that facilitates this process without damaging the oocyte.

Additional information

  • Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
    British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
  • Optimizations of an ovum pick up protocol in Donkey.
    Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
    https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
  • Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
  • Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
    Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Reproduction. Fertility and Development Sep;34(15):980-990
    https://doi.org/10.1071/RD22041
  • 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
    Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
    https://doi.org/10.1093/molehr/gaaa075
  • Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
    Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
    Methods X. vol 7, pp:1-9; 2020, 100767
    https://doi.org/10.1016/j.mex.2019.12.008
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U23-S02. Embryo culture media viability studies

Embryo culture media viability studies

Through this service, and through the time-lapse incubator that we have, embryonic development studies can be carried out by enriching the culture media with different compounds or additives to later verify which of them produces better embryonic development and better quality of the embryos, through viability and embryokinetic studies.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.

In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out quality control for Human Reproduction clinics, studies of in vitro fertilization and viability of embryo culture media, of mouse embryos or other animal species, whether that we have piezo electric. Furthermore, as we have a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

  • Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
    British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
  • Optimizations of an ovum pick up protocol in Donkey.
    Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
    https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
  • Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
  • Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
    Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Reproduction. Fertility and Development Sep;34(15):980-990
    https://doi.org/10.1071/RD22041
  • 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
    Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
    https://doi.org/10.1093/molehr/gaaa075
  • Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
    Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
    Methods X. vol 7, pp:1-9; 2020, 100767
    https://doi.org/10.1016/j.mex.2019.12.008
Read More

U23-S01. In vitro fertilisation studies and embryo culture media viability

In vitro fertilisation studies and embryo culture media viability

Through this service, in vitro fertilization processes can be carried out, including IVF and ICSI, of oocytes and sperm from different animal species, including mice, which are the animal species we mainly work with. To carry out this fertilization we have cabins, heated laminar flow, with stereomicroscopes included, plates, incubators with different gas systems and in order to evaluate the development of the embryos in real time we have recently incorporated a time lapse incubator, which allows us to carry out embryokinetic studies without having to remove the plates and the embryo from the incubator which improves the quality and viability of the embryo.

Customer benefits

Because our laboratory is one of the few that does research in assisted reproduction with animal models and has all this equipment, in addition to a bank of gametes and cryopreserved embryos, human reproduction clinics that wish to carry out tests with culture methods for oocytes or embryos, they can only be done with animal species, so our laboratory is suitable for this. In addition, the studies can also be carried out under regulatory requirements, since the institution that carries them out is certified in Good Laboratory Practices.
In this way, preclinical studies can be carried out in animal models in compliance with the strict guidelines of the regulatory agencies, ensuring the reliability and traceability of all the results and tests carried out in its different services.

Target customer

This unit can provide services to all those companies that want to carry out quality control for Human Reproduction clinics, studies of in vitro fertilization and viability of embryo culture media, of mouse embryos or other animal species, whether that we have piezo electric. Furthermore, as we have a bank of cryopreserved mouse oocytes and embryos, any company that needs to have a large number of these gametes and embryos can benefit from this service.

Additional information

  • Comparative pilot study of surgical activation vs application of platelet-rich plasma on ovarian function in an ovine animal model. P33. S Sánchez-Mateos, F Fabregues, E Párraga-Ros, I S Alvarez, J A Domínguez, M Méndez, J Ferreri, F M Sánchez Margallo.
    British Journal of Surgery, Volume 111, Issue Supplement_1, February 2024, znae018.033, https://doi.org/10.1093/bjs/znae018.033
  • Optimizations of an ovum pick up protocol in Donkey.
    Abstract publicado en la revista: Anim Reprod. 2022;19(2 spe):e22177
    https://www.animal-reproduction.org/journal/animreprod/article/62fe8d1fa9539502764c9db4
  • Supplementation of culture medium with quercetin improves mouse blastocyst quality and increases the expression of HIF-1α protein Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Zygote, 2023 Feb 27:1-12. https://doi:10.1017/S0967199423000060
  • Effect of the addition of 4OHE2 and quercetin in culture media on ROS levels and gene expression in mouse blastocysts
    Hernández, Nuria; Sánchez-Mateos, Soledad; López-Morató, Marta; Sánchez-Margallo, Francisco; Álvarez, Ignacio.
    Reproduction. Fertility and Development Sep;34(15):980-990
    https://doi.org/10.1071/RD22041
  • 4-Hydroxyestradiol improves mouse embryo quality, epidermal growth factor-binding capability in vitro, and implantation rates. Hernández N, López-Morató M, Perianes MJ, Sánchez-Mateos S, Casas-Rua V, Domínguez-Arroyo JA, Sánchez-Margallo FM, Álvarez IS.
    Mol Hum Reprod. Vol 27 (2) 2021 Nov 25:gaaa075.
    https://doi.org/10.1093/molehr/gaaa075
  • Histological cut of a paraffin-embedded blastocyst: optimized protocol for murine blastocysts.
    Alejandra Uson; Marta López-Morató; José Mijares; Soledad Sánchez-Mateos; Francisco Sánchez- Margallo; Ignacio Álvarez; Nuria Hernández, MSc
    Methods X. vol 7, pp:1-9; 2020, 100767
    https://doi.org/10.1016/j.mex.2019.12.008
Read More