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U6-S05. Freeze dryer

Freeze dryer

Freeze drying (also known as lyophilization) is a water (or other solvents) removal process typically used to preserve materials, with the goal of extending their shelf life or reducing its weight. Freeze drying works by freezing the material, then reducing the pressure and adding heat to allow the frozen water in the material to change directly to a vapor (sublimation).

Freeze drying occurs in three phases:

  1. Freezing: Freezing can be done in a freezer, a chilled bath (shell freezer) or on a shelf in the freeze dryer. Cooling the material below its triple point ensures that sublimation, rather than melting, will occur. This preserves its physical form.
  2. Primary Drying: Freeze drying’s second phase is primary drying (sublimation), in which the pressure is lowered and heat is added to the material in order for the water to sublimate. About 95% of the water in the material is removed in this phase. Primary drying can be a slow process.
  3. Secondary Drying: Freeze drying’s final phase is secondary drying (adsorption), during which the ionically-bound water molecules are removed. Most materials can be dried to 1-5% residual moisture.

Customer benefits

  • Solvent removal typically used to preserve materials, with the goal of extending their shelf life or reducing its weight.
  • The design of the equipment offers the best performance in the smallest possible space.
  • Equipment suitable for laboratories: compact and easy to install.
  • Technical reliability and excellent performance.
  • Ease of use: touch screen user interface.

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers
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U6-S06. Pulse-free microfluidic system

Pulse-free microfluidic system

Our device offers a pulse-free miniaturized platform designed to manipulate and control small volumes of fluids at the microscale, ranging from microliters to picoliters. The compact size, reduced sample consumption, faster reaction times, and potential for automation make microfluidic devices advantageous for a wide range of scientific and biomedical purposes.

Customer benefits

Offers precise handling of fluids within microchannels or microstructures, enabling various applications such as chemical analysis, biological assays, drug delivery, and point-of-care diagnostics.

Target customer

  • Chemical and biochemical companies.
  • Biology and chemistry research groups.
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U6-S07. Interactions of two biomolecules with respect to binding kinetics and affinity by surface plasmon resonance (SPR).

Interactions of two biomolecules with respect to binding kinetics and affinity by surface plasmon resonance (SPR).

Technically speaking, SPR refers to an optical phenomenon that enables monitoring of changes in refractive index via a quantum mechanical principle.

In a traditional SPR experiment:

  • A target is immobilized or captured onto a surface known as a sensor chip.
  • A pump is used to flow analytes over the sensor chip.
  • An optical measurement system captures changes occurring on the surface of the sensor chip.
  • Software plots time-dependent responses in the form of a graph called a sensorgram.

Customer benefits

With SPR, you can determine the rates and affinity of interactions between biomolecules and answer multiple questions using a single instrument.
SPR is the gold standard for analyzing biomolecules, providing:
High quality kinetics (association and dissociation constants, plus equilibrium)

  • Real-time data acquisition.
  • Label-free analysis.
  • Faster, automated experiments.
  • Lower sample consumption.

SPR’s flexibility lets you study biomolecular interactions in a wide variety of analytes, from small molecules in drug discovery to peptides, proteins, DNA, viruses, and even whole cells. SPR allows for:

  • Simple yes/no binding
  • Equilibrium studies
  • Complex kinetic analyses
  • Thermodynamic analysis
  • Concentration determination

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

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U6-S08. Tangential flow filtration

Tangential flow filtration

Tangential flow filtration (TFF) is a process of separation widely used in bio-pharmaceutical and food industries. It is different from other filtration systems in that the fluid is passed parallel to the filter, rather than being pushed through a membrane perpendicularly which can clog the filter media. This method is preferred for its continuous filtration and reproducible performance. The particles that pass through the membrane, the permeate, are put off to the side, while the rest, the retentate, is recycled back to the feed.

Customer benefits

Tangential flow filtration is used in the following processes:

  • Concentration: Increases the concentration of a solution by removing fluids while keeping the solute molecules. This process is done by selecting a filter significantly smaller than the solute molecules to allow for a higher retention of solute molecules.
  • Diafiltration: The separation of small and large particles, leaving the smaller particles behind without altering the overall concentration.

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

References

M.Köber, et al., J.C.I.S 2023, 631, 202-211

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U6-S09. Multimode plate reader

Multimode plate reader

The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable high-performance detection solutions empowered by monochromator- or filter-based technologies. The six new tailored configurations provide excellence in ELISA assays, nucleic acid quantifications, reporter assay technologies, and drug discovery assays including HTRF® and fluorescence polarization.
Dual-mode plate reader with monochromator-based optics for absorbance and sensitive fluorescence (top and bottom reading) applications. Your adjustable tool, even for low concentration nucleic acid and protein quantification.
The Infinite M Nano+ has an excitation monochromator optimized for wavelength accuracy and precision, ensuring excellent performance for every absorbance and fluorescence assay.Engineered for absorbance and fluorescence measurements, the system’s highly sensitive Quad4 Monochromators™ minimize stray light, delivering exceptional flexibility with sensitivity levels close to comparatively priced filter-based instruments.

Customer benefits

Intuitive, workflow-oriented software (i-control) which allows you to create a workflow for each application, using ‘drag and drop’ processing steps to generate your assay protocol, which can be saved for future use.

Highlights:

  • Real-time export data
  • Extended dynamic range
  • Automated z-focusing

Key applications:

  • Absorbance-based DNA/RNA quantification and purity checks
  • Fluorescence-based DNA/RNA quantification (PicoGreen, RiboGreen®)
  • Absorbance-based protein quantification (BCA, Bradford, Lowry, etc.)
  • Fluorescence-based protein quantification (eg. NanoOrange®)
  • Absorbance- and fluorescence-based ELISAs600 nm growth curves (bacteria, yeast)
  • Enzyme kineticsCompound characterization

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

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U6-S02. High-pressure phase analysis – solubility, emulsification (Remote) OUTSTANDING

High-pressure phase analysis – solubility, emulsification.

This phase equilibria unit is built for the measurement and detection of phase equilibria and phase transitions by optical means.
The mixture of solute and solvent gas is agitated by the magnetic stirrer. Whenever samples are drawn from the top or the bottom connection in the cell, the directly connected counterbalance piston moves towards the centre of the cell, this keeping the pressure in the measuring cell constant even during the sampling operation. No need to add additional solvent gas, which would change mass ratio and temperature and consequently result in a disturbed equilibrium.

Customer benefits

Phase equilibria cell:
• Capacity: 29-55 mL (depending on piston position)
• Operating pressure max.: 200 Bar
• Operating temperature max.: 150 °C

Counterbalance piston to maintain constant pressure during sampling operation
• Optical windows: 2x ø28mm (sapphire)
• Optical path length: 58 mm

Double wall heating jacket for heating with external thermostat.
High-pressure thermocouple type K (inner temperature)

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

References

N-Grimaldi, et al. ACS Nano 2017, 11, 10774-10784

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U6-S01. Use of High-pressure laboratory-scale plant

Use of High-pressure laboratory-scale plant

with 50, 100 and 300 mL reactors for the processing of biomaterials. Processing of cytotoxic compounds when required.

Preparation of soft molecular materials with controlled structure at supramolecular, micro- and nanoscopic level, using one-step methodologies based on green compressed fluids (i.e. compressed and supercritical CO2).Micro- and nanoparticulate single compounds with high supramolecular homogeneity (i.e., pure polymorphic phases, materials with single polymer folding, etc.).

  • Particulate polymeric matrix uniformly loaded with active compounds (therapeutics, cosmetic ingredients, catalyst, pigments, and dyes, etc.)
  • Dispersed systems (suspensions, liposomes, emulsions, vesicles) with narrow particle size distribution and high morphological homogeneity.
  • Porous materials, either crystalline or amorphous, with defined porosity and porous size.

Customer benefits

Lab-scale high pressure systems, based on a 50 mL, a 100 mL and a 300 mL stirred high pressure autoclaves equipped with pumps for the supply of compressed fluids and liquid solutions. The high-pressure systems can also optionally be equipped with several filters, manometers, thermocouples, and back pressure regulators. The maximum operative pressure is 23 MPa and the maximum operative temperature is 200 °C.
The 300 mL system is also equipped with a mass flowmeter, and a data acquisition system.
All the plants have been designed for micro- and nanostructuring molecular and soft materials.

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

References

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U3-S02. Post synthesis peptide modification (On-site&Remote) OUTSTANDING

Post synthesis peptide modification (On-site&Remote) OUTSTANDING

Post-synthesis peptide modification such as:
– Cyclization through a disulphide bridge, amide, ester, thiosester, thioether, or various staple bonds.
– Biotinylation and/or incorporation of various imaging probes at the N-terminus, C-terminus or on some amino acid side chains (Lys, Cys…).
– Convenient peptide derivatisation for further conjugation by introducing a maleimide, succinimidyl, alkyne or azide-containing spacers.
– Peptide conjugation to polymers, proteins, probes and small molecules.
Multivalent peptide presentation molecules.

Customer benefits

  • Extensive experience in the synthesis of cyclic peptides (disulfide bridge, lactam, lactone, thioester, thioether).
  • Extensive experience in the synthesis of stapled peptides
  • Development of several peptide cyclization methods.
  • Extensive experience in the derivatisation of peptides for conjugation and attachment to various types of molecules (nanoparticles, polymers, imaging probes, small molecules).
  • Development of various strategies for the introduction of post-synthesis modifications.
  • Development of conjugation methodology.
  • Development of methodology for the generation of multivalent peptide presentation molecules.

Target customer

  • Research groups (drug delivery, molecular biology, pharmacology, nanotechnology, biotechnology)
  • Companies (biotech and pharma companies).

References

  • Direct Quantitative Immunochemical Analysis of Autoinducer Peptide IV for Diagnosing and Stratifying Staphylococcus aureus Infections. Montagut, Enrique-J.; Acosta, Gerardo; Albericio, Fernando; Royo, Miriam; Godoy-Tena, Gerard; Lacoma, Alicia ; Prat, Cristina ; Salvador, Juan-Pablo; Marco, Maria-Pilar. ACS Infectious Diseases (2022), 8, 645-656.
  • Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion. Martinez-Miguel, Marc; Castellote-Borrell, Miquel; Kober, Mariana; Kyvik, Adriana R. ; Tomsen-Melero, Judit ; Vargas-Nadal, Guillem; Munoz, Jose; Pulido, Daniel ; Cristobal-Lecina, Edgar; Passemard, Solene; oyo, Miriam ; Mas-Torrent, Marta ; Veciana, Jaume ; Giannotti, Marina I. ; Guasch, Judith ; Ventosa, Nora ; Ratera, Imma. ACS Applied Materials & Interfaces (2022), 14, 48179-48193.
  • Engineering a Nanostructured Nucleolin-Binding Peptide for Intracellular Drug Delivery in Triple-Negative Breast Cancer Stem Cells. Pesarrodona, Mireia; Sanchez-Garcia, Laura; Seras-Franzoso, Joaquin; Sanchez-Chardi, Alejandro; Balta-Foix, Ricardo; Camara-Sanchez, Patricia; Gener, Petra; Jara, Jose Juan; Pulido, Daniel ; Serna, Naroa; Schwartz, Simo; Royo, Miriam ; Villaverde, Antonio; Abasolo, Ibane ; Vazquez, Esther. ACS Applied Materials & Interfaces (2020), 12, 5381-5388.
  • Synthesis of Stable Cholesteryl-Polyethylene Glycol-Peptide Conjugates with Non-Disperse Polyethylene Glycol Lengths. Cristobal-Lecina, Edgar; Pulido, Daniel; Martin-Malpartida, Pau; Macias, Maria J.; Albericio, Fernando; Royo, Miriam. ACS Omega (2020), 5, 5508-5519.
  • Highly Versatile Polyelectrolyte Complexes for Improving the Enzyme Replacement Therapy of Lysosomal Storage Disorders. Giannotti, Marina I.; Abasolo, Ibane; Oliva, Mireia; Andrade, Fernanda; Garcia-Aranda, Natalia; Melgarejo, Marta; Pulido, Daniel; Corchero, Jose L.; Fernandez, Yolanda; Villaverde, Antonio; Royo, Miriam; Garcia-Parajo, Maria F.; Sanz, Fausto; Schwartz, Simo. ACS Applied Materials & Interfaces (2016), 8(39), 25741-25752.
  • Gated mesoporous silica nanoparticles using a double-role circular peptide for the controlled and target-preferential release of doxorubicin in CXCR4-expressing lymphoma cells. de la Torre, Cristina; Casanova, Isolda; Acosta, Gerardo; Coll, Carmen; Moreno, Maria Jose; Albericio, Fernando; Aznar, Elena; Mangues, Ramon; Royo, Miriam; Sancenon, Felix; Martinez-Manez, Ramon. Advanced Functional Materials (2015), 25, 687-695.
  • Multivalent dendrimers presenting spatially controlled clusters of binding epitopes in thermoresponsive hyaluronan hydrogels. Seelbach, Ryan J.; Fransen, Peter; Peroglio, Marianna; Pulido, Daniel; Lopez-Chicon, Patricia; Duttenhoefer, Fabian ; Sauerbier, Sebastian; Freiman, Thomas; Niemeyer, Philipp; Semino, Carlos; Albericio, Fernando; Alini, Mauro; Royo, Miriam; Mata, Alvaro; Eglin, David. Acta Biomaterialia (2014), 10, 4340-4350
  • Triazene as a powerful tool for solid-phase derivatization of phenylalanine containing peptides: Zygosporamide analogues as a proof of concept. Torres-Garcia, Carolina; Pulido, Daniel; Albericio, Fernando; Royo, Miriam; Nicolas, Ernesto. Journal of Organic Chemistry (2014), 79(23), 11409-11415.

3. Peptides-System for acidolactic cleavage of the peptide resin boundby anhydrous HF
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U3-S01. Synthesis of peptides and characterisation (On-site&Remote) OUTSTANDING

Synthesis of peptides and characterisation (On-site&Remote) OUTSTANDING

Peptide production on various scales from mg (scale: 10 mg, 50 mg and 100 mg) and grams.
Synthesis of peptides containing phosphorylated amino acids, acylated or methylated side chains (lysine) or non-natural amino acids.
Peptides can be delivered as synthesis crudes (without purification) and purified (≥95% by HPLC).
Peptides are characterized by HPLC and HPLC-MS

Customer benefits

  • Extensive experience on peptide synthesis.
  • Experience in difficult sequences.
  • Control quality at different synthesis points.
  • Capability to design diverse synthesis strategies and purification
  • Development of peptide synthesis methodology.

Target customer

  • Research groups (drug delivery, molecular biology, pharmacology, nanotechnology, biotechnology)
  • Companies (biotech and pharma companies).

References

  • Pharmacological activation of insulin-degrading enzyme improves insulin secretion and glucose tolerance in diet-induced obese mice. Sanz-Gonzalez, Alba; Cozar-Castellano, Irene; Broca, Christophe ; Sabatier, Julia; Acosta, Gerardo A. ; Royo, Miriam ; Hernando-Munoz, Carla; Torroba, Tomas ; Perdomo, German ; Merino, Beatriz. Diabetes, Obesity and Metabolism (2023), 25, 3268-3278.
  • Amide Formation: Choosing the Safer Carbodiimide in Combination with OxymaPure to Avoid HCN Release. Manne, Srinivasa Rao; Luna, Omar; Acosta, Gerardo A.; Royo, Miriam ; El-Faham, Ayman ; Orosz, Gyorgy; de la Torre, Beatriz G. ; Albericio, Fernando. Organic Letters (2021), 23, 6900-6904.
  • Carbosilane Dendron-Peptide Nanoconjugates as Antimicrobial Agents
  • By: Fernandez, Jael; Acosta, Gerardo; Pulido, Daniel; Maly, Marek; Copa-Patino, Jose L.; Soliveri, Juan; Royo, Miriam; Gomez, Rafael; Albericio, Fernando; Ortega, Paula; de la Mata, F. Javier. Molecular Pharmaceutics (2019), 16, 2661-2674.
  • Optimized Stepwise Synthesis of the API Liraglutide Using BAL Resin and Pseudoprolines. Carbajo, Daniel; El-Faham, Ayman; Royo, Miriam; Albericio, Fernando. ACS Omega (2019), 4, 8674-8680
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U2-S01. Scientific and technical support

Scientific and technical support

The scientific and technical support service provides valuable assistance to researchers and organizations in various aspects:

  • Immunogen Design: Expert advice on designing effective immunogens for antibody production.
  • ­Immunoreagent Synthesis and Production: Guidance on synthesizing and producing immunoreagents, including monoclonal and polyclonal antibodies.
  • Antibody Production: Support for generating high-quality antibodies for research purposes.
  • Immunoassay Design and Development: Customized development of immunoassays, including assay format design, sample matrix considerations, and sensitivity optimization.

The technical support provided is focused on identifying suitable immunogens for antibody production with desired characteristics for the user, while in the antibody production section, support is oriented towards designing screening methods during monoclonal antibody development to search for hybridomas with the desired specificity and sensitivity characteristics for each user.

Customer benefits

  • Scientific Expertise: Access to specialized knowledge in immunology and immunochemistry.
  • Technical Guidance: Assistance in experimental design, troubleshooting and optimization.
  • Cost-Effective Solutions: Avoiding the need to establish in-house facilities for antibody production and immunoassay development.
  • Accelerated Research: Faster progress due to expert support and streamlined processes.
  • ISO 9001 Certification: The development and production of monoclonal antibodies is backed by the ISO 9001:2015 certification, ensuring quality, reliability and adherence to international standards.

Target customer

Scientific and Technical Support Serviceis essential for organizations involved in Research and Development (R&D) across fields such as food safety and environmental control,  biomedicine, diagnostics, drug discovery, and therapeutic development.

Additional information

Selected references:

  • B. Rodriguez-Urretavizcaya, N. Pascual, C. Pastells, M. T. Martin-Gomez, Ll. Vilaplana, M.-P. Marco. Diagnostic and Stratification of Pseudomonas aeruginosa Infected Patients by Immunochemical Quantitative Determination of Pyocyanin from Clinical Bacterial Isolates. Frontiers in Cell. Infect. Microbiol., 11, 786929, 2021. DOI: 10.3389/fcimb.2021.786929. URL
  • Giovanna Roncador; Pablo Engel; Lorena Maestre; et al; Alison H.Banham., Nuria Pascual 2016. The European antibody network’s practical guide to finding and validating suitable antibodies for research. mAbs. Taylor & Francis Online. 8-1, pp.27-36.
  • Carme Pastells; Gerardo Acosta; Nuria Pascual; Fernando Albericio, Miriam Royo; M.-Pilar Marco. 2015. An immunochemical strategy based on peptidoglycan synthetic peptide epitopes to diagnose Staphylococcus aureus infections. Analytica Chimica Acta. Elsevier. 889, pp.203-211
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