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U14-E01. Cell culture rooms

Cell Culture Room 1:
MSC Advantage Class II b Biological Safety Cabinet (Thermo)
CO2 incubator Heracell 150 (Thermo)
Nikon Eclipse TE2000 fluorescence inverted microscope (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)

Cell Culture Room 2:
2 MSC Advantage Class II b Biological Safety Cabinets (Thermo)
2 CO2 incubators Heracell 150 (Thermo)
Phases inverted microscope (NIKON)
2 Vacuum Pumps ME4 (Vacuubrand)
Cell Counter (Invitrogen)

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U14-E02. Molecular biology

MACSQuant 10 Analizer Flow Cytometer (Miltenyi Biotec)

Microplate reader Synergy MX (BIOTEK)

QuantStudio 3 Real-Time PCR System (AppliedBiosistem)

UV/Vis Spectrophotometer (IMPLEN)

Thermal Cycler PXE 0.2 (THERMO)

Fume Hood Nublar NBL-1600 (Formimetal)

Refrigerated centrifugeMultifuge 1 (Heraeus)

Refrigerated centrifuge for microtubes Heraeus Fresco 21 (Thermo)

Rotary shaker with incubation (HEIDOLPH)

Digital circulation thermostatic bath (GRANT)

Manual variable volume pipettes (Eppendorf, Kartell, Rainin)

Multichannel variable volume pipettes (Thermo)

Pipette controllers (Gosselin, Rainin, Lab box)

Liquid Nitrogen tank (Taylor Wharton)

Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80°C) (HERAEUS)

Freezers -20°C (FIOCHETTI, Indesic)

Refrigerators +2/+8ºC (Thermo, CLASS ELECTRONIC)

Thermoblock Accublock (Labnet)

Heating magnetic stirrer ARE (VELP Scientifica)

Analytical balance (Metria)

Precision analytical balance Pioneer (OHAUS)

pH-Meter Symphony (VWR)

Ultrasonic water bath (SELECTA)

Vortex 230 V, EU (VWR)

Microwave (LG)

Crushed ice machine (Schottman)

Autoclave Presoclave-II (P SELECTA)

Sterilization incubator (Modelo UT ) (HERAEUS)

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U13-E02. Machines for mechanical tests

Machines for mechanical tests:

• Instron MicroTester 5848 and 5548: electromechanical machines for displacement or load control tests:

•• load range from 1mN to 2kN at full scale.
•• load cells of:+/- 5, 50, 500 and 1000N.
•• synchronous data capture in all the channels up to
500 Hz.
• Biaxial Instron MicroTester 8874: axial-torsional, servohydraulic testing machine:
•• load capacity of 25 kN and 100 Nm.
•• fatigue tests at a low number of cycles.

›› Software for data control and analysis.
›› Accessories and tools especially designed by the group, which allow a wide flexibility in designing tests, allowing the verification and/or validation of theoretical models.
›› AVE video extensometer connected to the machines; resolution of 0.5 μm. To determine the axial and transverse deformation without contact (tension, dynamic properties and viscoelasticity) of the samples.

• E1000 Test Instrument: Dynamic systems to offer slow-speed static testing and high-frequency dynamic fatigue testing with hundreds of Hertz capability: Load range: 250 N to 2.0 KN.
• Nano BIONIX MTS: For nanomechanical characterization of biomaterials. Load range to 0.001N to 0.5N.

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U13-E01. MicroCT GE eXplore Locus SP (GE)

MicroCT GE eXplore Locus SP (GE): Computed X-ray Axial Tomograph for the non-destructive study of the microstructure and in vitro tomography: ›› Samples of up to 40 mm in diameter; resolution of 10 μm. ›› Different types of tissues, implants, scaffolds, etc. ›› Complete “section-by-section” imaging with a single rotation (cone beam volumetric technology); excellent image quality and “signal-to-noise” ratio. ›› “Bone Analysis Tool” software provided with: •• BMD (Bone Mineral Density): It calculates the density of minerals in the bone, the bone volume fraction and the mineral content in the bone. •• Anisotropy: It determines the degree of symmetry and the orientation of the trabecular structure. •• Cortical Analysis: It determines “section-by-section” the width, surface area and the BMD value of the cortical bone. •• Stereology: It provides the bone volume fraction, the ratio between the surface of the bone and its volume and the width of the trabecular plate. •• Direct Measures: It determines the local trabecular width of a bone and allows obtaining a visual representation of this local width.

Temporarily OUT OF ORDER

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U13-S01. MicroCT

MicroCT

X-Ray image for biomaterials, scaffolds and biological tissues.

Customer benefits

An axis inside is able to apply forces.
Non-destructive view of internal structure pre and post force.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

Additional information

C:\Users\usuario\AppData\Local\Temp\snapshot.jpg

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U13-S03. Fatigue tests Instron 8874

Fatigue tests Instron 8874

Fatigue tests allow us to know how is the behaviour when cyclic load are applied. It is used for life expectancy of biomaterials and biological tissues.

Customer benefits

High full-scale cell load.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

Additional information

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U13-S04. Dynamic mechanical analysis Instron 8874

Dynamic mechanical analysis Instron 8874

Dynamic tests allow us to know how is the behaviour along time. It is used for viscoelastic properties of biomaterials and biological tissues.

Customer benefits

High full-scale cell load.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

Additional information

D:\gestion\Laboratorio\espacios\fotos_plat\INSTRON 8874\P1000356.JPG

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U13-S05. Uniaxial test Instron MicroTester

Uniaxial test Instron MicroTester

Tensile, pure shear and planar tension are the tests inside the uniaxial group. Samples are stretched along an axis by measuring force and displacement. Cycling protocols can be integrated into the mechanical test. Mechanical and viscoelastic properties can be studied. Force is measured by means of load cells.

Customer benefits

  • Load cells are available with different full-scales depending on the material to test.
  • Tests can be controlled by means of displacement or force.
  • Specific protocols according customer requirements.
  • Tracking system can be used to measure real deformations.
  • Adaptable clamps according to sample geometry.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

References

  • Ríos-Ruiz, I., Martínez, M.Á. & Peña, E. Is location a significant parameter in the layer dependent dissection properties of the aorta?. Biomech Model Mechanobiol 21, 1887–1901 (2022). Doi: 10.1007/s10237-022-01627-9
  • Peña E, Alastrué V, Laborda A, Martínez MA, Doblaré M. A constitutive formulation of vascular tissue mechanics including viscoelasticity and softening behaviour. J Biomech. 2010 Mar 22;43(5):984-9. doi: 10.1016/j.jbiomech.2009.10.046. Epub 2009 Dec 2. PMID: 19959171.

Additional information

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U13-S06. Confined compression tests Instron MicroTester

Confined compression tests Instron MicroTester

Samples are compressed along an axis by measuring force and displacement. Cycling protocols can be integrated into the mechanical test. Mechanical and viscoelastic properties can be studied. Force is measured by means of load cells.

Customer benefits

  • Load cells are available with different full-sales depending on the material to test.
  • Tests can be controlled by means of displacement or force.
  • Specific protocols according customer requirements.
  • Adaptable system according to sample geometry.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

References

I. Cabeza-Gil, I. Ríos-Ruiz, B. Calvo, Experimental evaluation of the injection force exerted in intraocular lens delivery with syringe-type injectors,
Journal of the Mechanical Behavior of Biomedical Materials, 124, 2021, 104793, ISSN 1751-6161, Doi: 10.1016/j.jmbbm.2021.104793.

Additional information

D:\gestion\Laboratorio\espacios\fotos_plat\INSTRON 5548\Instron 5548-m.jpg

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U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

Description:
High-speed centrifuge with aluminum rotors at fixed angle – Rotor JA-25.15 (maximum centrifugal force = 74200g, maximum speed = 25000 rpm, capacity for 24 tubes of 15 mL each, total capacity = 360 mL) – Rotor JLA-16.250 (maximum centrifugal force = 38500g, maximum speed = 16000 rpm, capacity for 6 tubes of 250 mL each, total capacity = 1500 mL) – Rotor JA-10 (maximum centrifugal force = 17700g, maximum speed = 10000 rpm, capacity for 6 tubes of 500 mL each, total capacity = 3 L)

Applications:
It allows to centrifuge samples such as emulsions, dispersions, etc at angular velocities up to 25000 rpm (74200 g) at various temperatures (from -20˚C a 40˚C), which can be used for phase separation, stability studies, etc. in colloidal systems and for the separation of cells and proteins.

U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)
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